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EC number: 700-179-1 | CAS number: 5128-28-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 June 1992 to 16 July 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: some deviations to GLP and testing guideline, but adequate coherence between data, c omments and conclusions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- total of 2 doses although lethal at 2000 mg/kg (no DL50 calculated); but this guideline has been deleted
- GLP compliance:
- yes
- Remarks:
- no laboratory GLP certificate, no analytical certificate
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 4,6-dinitro-2,1λ⁵,3-benzoxadiazol-1-one
- EC Number:
- 700-179-1
- Cas Number:
- 5128-28-9
- Molecular formula:
- C6H2N4O6
- IUPAC Name:
- 4,6-dinitro-2,1λ⁵,3-benzoxadiazol-1-one
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: breeder
- Age at study initiation: 6 weeks
- Mean weight at study initiation: 185 g for males and 157 g for females
- Fasting period before study: 18 hours
- Housing: collective
- Diet: conventional laboratory diet, ad libitum
- Water : filtered water, ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3°C
- Humidity (%): 50 +/- 20%
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12 h / 12 h
IN-LIFE DATES: From: 18 June 1992 To: 16 July 1992
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- DMSO
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20% (dose-level of 2000 mg/kg) and 2% (dose-level of 200 mg/kg)
- Justification for choice of vehicle: no justification, the 20% solution in DMSO was provided by the
Sponsor
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: no toxic data were available, consequently the
starting dose was 2000 mg/kg - Doses:
- 200 and 2000 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- Details on study design
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: at least once daily for clinical signs and on days 1, 5, 8
and 15 for weighing
- Necropsy of survivors performed: yes
- Other examinations performed: none
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 200 - < 2 000 mg/kg bw
- Mortality:
- At 200 mg/kg, no mortality was observed.
At 2000 mg/kg, all animals were found dead 24 hours after treatment. - Clinical signs:
- other: At 200 mg/kg: hypokinesia, dyspnea, hypersalivation and sedation were observed in all animals in the minutes following the treatment and in some cases up to day 3. No clinical signs were observed bet ween day 4 and day 15. At 2000 mg/kg: dyspnea, hypersal
- Gross pathology:
- In the animals found dead, brownish stomach mucosis was observed, probably due to haemorrhagic lesions.
Applicant's summary and conclusion
- Conclusions:
- The LD50 of the substance in rats after single oral administration was comprised between 200 and
2000 mg/kg.
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