Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 260-847-9 | CAS number: 57609-64-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Chemical analysis of the test item concentrations in the test solutions was performed to determine
exposure concentration and stability of the test item during exposure. Substance-specific analysis
was performed at the test site menal GmbH.
After 0 h, 24 h, 48 h and 72 h exposure, both additional vessels of NC, A, B, C, D and E were
sampled; one sample per replicate: 2 samples of 5 mL per treatment group. The samples were
filled into 12 mL glass vials. To each vial, 5 mL acetonitrile was added and samples were stored in
the freezer (≤ -18 °C).
Of each sampled treatment, one of the analytic samples from 0 h, 24 h, 48 h and 72 h was sent to
the analytical laboratory at the test site menal GmbH for chemical analysis. The remaining samples
were stored as retain samples in the freezer (≤ -18 °C) until finalisation of the study. - Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test organism Raphidocelis subcapitata (Strain No. 61.81 SAG) originates from the Culture
Collection of Algae at the University of Goettingen and was cultivated in suspension culture. The
strain used for this study has been cultured at Hydrotox GmbH since July 2017. Twice a week, the
stock suspension is inoculated into fresh Holm-Hansen medium (composition: 496 mg/L NaNO3,
39 mg/L K2HPO4, 75 mg/L MgSO4×7H2O, 36 mg/L CaCl2×2H2O, 58 mg/L Na2CO3, 10 mg/L
Na2EDTA×2H2O, 3 mg/L citric acid, 3 mg/L iron citrate, 0.1144 mg/L H3BO3, 0.0724 mg/L MnCl2×
4H2O, 0.0088 mg/L ZnSO4×7H2O, 0.0032 mg/L CuSO4×5H2O, 0.0010 mg/L Na2MoO4×2H2O,
0.0016 mg/L CoCl2×6H2O) under axenic conditions to keep it in exponential growth. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Details on test conditions:
- The test vessels are sealed with cellulose stoppers and kept in the light incubator (specified
temperature: 21 – 24 °C, constant within ± 2 °C). They are placed on the shaking table with adhesive
mats and continuously shaken with 100 rpm and an orbital diameter of 5 cm.
The test vessels were permanent illuminated by LEDs (warm white) with a light intensity of 88.5 μE m
² s-1 ± 8.0 %. The pH was measured at the start (0 h) and at the end (72 h) of the test. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.019 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.501 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.159 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
EC50 (Yield, 72 h): 0.019 mg/L, 95 % Confidence Limit (CL): 0.007 – 0.053 mg/L
EC50 (Growth rate, 72 h): 3.501 mg/L, 95 % Confidence Limit (CL): 0.627 – 18.398
EC10 (Yield, 72 h): 0.0 mg/L (n.d.), 95 % Confidence Limit (CL): 0.000 – 0.000 mg/L
EC10 (Growth rate, 72 h): 0.159 mg/L, 95 % Confidence Limit (CL): 0.040 – 0.625 mg/L- Executive summary:
EC50 (Yield, 72 h): 0.019 mg/L, 95 % Confidence Limit (CL): 0.007 – 0.053 mg/L
EC50 (Growth rate, 72 h): 3.501 mg/L, 95 % Confidence Limit (CL): 0.627 – 18.398
EC10 (Yield, 72 h): 0.0 mg/L (n.d.), 95 % Confidence Limit (CL): 0.000 – 0.000 mg/L
EC10 (Growth rate, 72 h): 0.159 mg/L, 95 % Confidence Limit (CL): 0.040 – 0.625 mg/L
Reference
Description of key information
95 % Confidence Limits (CL) Yield (72 h) Growth rate (72 h)
EC50 (Yield, 72 h): 0.019 mg/L, 95 % Confidence Limit (CL): 0.007 – 0.053 mg/L
EC50 (Growth rate, 72 h): 3.501 mg/L, 95 % Confidence Limit (CL): 0.627 – 18.398
EC10 (Yield, 72 h): 0.0 mg/L (n.d.), 95 % Confidence Limit (CL): 0.000 – 0.000 mg/L
EC10 (Growth rate, 72 h): 0.159 mg/L, 95 % Confidence Limit (CL): 0.040 – 0.625 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 3.501 mg/L
- EC10 or NOEC for freshwater algae:
- 0.159 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.