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EC number: 205-858-1 | CAS number: 156-57-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 2018 - May 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- yes
- Remarks:
- Stock solution, used for ISO water preparation, are 5 times more concentrated than OECD 202 annex III.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10, 4 , 1 mg/L
- Sampling method: aliquots were taken
- Sample storage conditions before analysis: 4±2°C for all determination except pH determinations (directly carried). - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Starting from the 100 mg/L SOLUTION in ISO test water, following test solutions in ISO test water were prepared: 1, 1.6, 2.5, 4, 6.3, 10 mg/L.
100 mg/L SOLUTION in ISO test water was prepared new for each test
- Eluate: NO
- Controls: ISO TEST SOLUTION without test substance
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): NO - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus 1820
- Source: Breeding of a certified Daphtokit
- Age of parental stock: 30 days
- Feeding during test: NO
ACCLIMATION
- Acclimation period: continuos breeding
- Acclimation conditions (same as test or not): same as test excluding light intensity (1000 ± 20% lux) and breeding water (mineral wayer similar for chemical composition to ISO test water)
- Type and amount of food: green algae (Pseudokirchneriella subcapitata) and brewer's yeast; 300000 cells/ml
- Feeding frequency: every day
- Health during acclimation (any mortality observed): no mortality observed
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 240 g/L of CaCO3
- Test temperature:
- 20±1°C (taverage 20°C; tmin 19.9°C; tmax 20.1°C)
- pH:
- 8.00÷8.70
- Dissolved oxygen:
- 8.60-8.74 mg/L
- Nominal and measured concentrations:
- 1 (measured 1.39), 1.6, 2.5, 4 (measured 5.47), 6.3, 10 (measured 9.76) mg/L.
Standard: Mercaptamine hydrochloride 50 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 ml glass beaker filled with 50 ml of sample
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: iso stock solutions
- Culture medium different from test medium: Culture medium is a mineral water with little differences in chemical composition than test medium
- Intervals of water quality measurement: every new batch of culture medium is controlled; test medium is controlled at every use
OTHER TEST CONDITIONS
- Photoperiod: 16h luce e 8h buio
- Light intensity:300±20% lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of immobilized daphnids observed at 24 and 48h
VEHICLE CONTROL PERFORMED: no vehicle
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: yes; provisional 48hEC50 turned out to be between 1 and 10 mg/l - Reference substance (positive control):
- yes
- Remarks:
- Potassium Chloride
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.75 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: NO
- Observations on body length and weight: NO
- Other biological observations:NO
- Mortality of control:NO
- Other adverse effects control:NO
- Abnormal responses:NO
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:NO - Reported statistics and error estimates:
- Statistical processing of test results was performed using a probit test contained in the EPA software indicated in the US EPA “Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms” – Fifth edition - october 2002.
The same software returns the value of 95% confidence limits - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h acute toxicity of Mercaptamine HCl to Daphnia magna was studied under static conditions. Daphnids were exposed to ISO test water control and test chemical at measure concentration of 1, 1.6, 2.5, 4, 6.3, 10 mg/L of test substance for 48h.
Immobilization sublethal effects were observed daily.
The 48h EC50 was 2.75 mg/L (LC95%: 2.38-3.18 mg/l).
No sublethal effects were observed.
Based on the results, Mercaptamine HCl would be consider as NO toxic to invertebrates.
This study is classified as reliability 1 and satisfies the guideline requirements for an acute toxicity with freshwater invertebrates.
Reference
CONTROL | 1mg/L | 1.6mg/L | 2.5mg/L | 4mg/L | 6.3mg/L | 10mg/L | |
1 | 0 | 0 | 1 | 2 | 5 | 5 | 5 |
2 | 0 | 0 | 1 | 2 | 3 | 5 | 5 |
3 | 0 | 0 | 0 | 2 | 4 | 5 | 5 |
4 | 0 | 0 | 0 | 2 | 4 | 5 | 5 |
IMMOBILIZATION (TOTAL) | 0 | 0 | 2 | 8 | 16 | 20 | 20 |
TOTAL EXPOSED | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
% OF IMMOBILIZED | 0 | 0 | 10 | 40 | 80 | 100 | 100 |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.75 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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