Acetoacetamide

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 November 2017 - 17 November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

25°C

Details on study design: HPLC method:

HPLC System: Agilent 1260 HPLC system
Column: EC 150/4.6 Nucleodur 100-5 CN-RP 150 x 4.6 mm; 5 1-1m
The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid
phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix
was used (EC 150/4.6 Nucleodur 100-5 CN-RP 150 x 4.6 mm; 5 !Jm).
A of 55 % methanol I 45 % ultra-pure grade water was used as mobile phase and the column temperature
was 25°C ± 1 °C.

Analytical monitoring:

no

Key result

Sample No.:

#1

Type:

log Koc

Value:

ca. 1.32 dimensionless

Temp.:

25 °C

Matrix:

Silica matric (HPLC)

Remarks on result:

other: HPLC method

Remarks:

pH value not measured

Details on results (HPLC method):

The log Koc values were below the lowest used reference standard (log Koc of the lowest reference
standard phenol is 1.32). Therefore the repeatability could not be determined.

Validity criteria fulfilled:

yes

Conclusions:

The log Koc values were below the lowest used reference standard (log Koc of the lowest reference standard phenol is 1.32).
Therefore, the log Koc is < 1.32.

Executive summary:

The aim of this study was the determination of the adsorption coefficient of Acetoacetamide on soil and on sewage sludge (Koc) by high performance liquid chromatography (HPLC) method according to EC method

C.19 (440/2008) and OECD guideline 121.

The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix

was used. A of 55 % methanol / 45 % ultra-pure grade water was used as mobile phase and the column temperature was 25°C ± 1 °C.

The log Koc values were below the lowest used reference standard (log Koc of the lowest reference standard phenol is 1.32).

Therefore, the log Koc is < 1.32.

Description of key information

The log Koc values were below the lowest used reference standard (log Koc of the lowest reference standard phenol is 1.32).

Therefore, the log Koc is < 1.32.