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EC number: 274-958-5 | CAS number: 70865-30-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- June from 02nd to 22nd, 2014
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- Justification for read across apporach is detailed in the report attached to the IUCLID section 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical samples were taken and analysed from 100 mg/l and control at test start (fresh) and after 48 hours (aged).
- Details on test solutions:
- The necessary amount of test item for preparing the test solution was weighed on weighing scoops and transferred to a volumetric flask.
Test medium was added up to the bench mark and the solution was homogenised by shaking. 50 ml of the prepared solution was transferred to each test vessel. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain/clone: Daphnia magna Straus, Clone V.
- Source: the animals are continuously bred in the laboratory and were originally purchased in a healthy condition from the Umweltbundesamt (Federal Environment Agency) in Berlin/Germany.
- Age of parental stock: freshly hatched daphnids less than 24 hours old were used for the test.
- Feeding during test: none.
- Food: fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus).
- Frequency: at least three times a week.
BREEDING CONDITIONS
- Medium: organisms were bred as group culture (5 to 20 daphnids per litre) in Elendt M4 medium.
- Temperature: daphnids were reared at 20 ± 2 °C in a climatic chamber.
- pH: aerated water was within a range of 6.0 – 9.0.
- Dissolved oxygen: above 60 % saturation.
- Total hardness 140 - 250 mg/l (as CaCO3), corresponding to 7.8 - 14°dH.
- Photoperiod: 16 hours of illumination and 8 hours of darkness.
- Renewal of the medium: the medium was changed three times per week. A pipette was used to separate the young daphnids from the adults. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 20.6 ± 0.2 °C
- Dissolved oxygen:
- 8.8 ± 0.1 mg/l
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: four 100 ml glass beakers per concentration, each filled with 50 ml, one additional replicate for measurements without test organisms.
- Aeration: none.
- No. of organisms per concentration: 20.
- No. of vessels per concentration: 4 replicates of 5 animals each.
- No. of vessels per control: 4 replicates.
- No. of vessels per reference substance: 1 replicate.
- Loading rate: 10 ml of test solution for each animal.
TEST MEDIUM / WATER PARAMETERS
- Test medium: Elendt M4 medium.
- pH: at test initiation the pH-value of the control (untreated test medium) was 7.96.
- Dissolved oxygen: at test initiation the concentration in the control was 8.9 mg/l.
- Total hardness: at test initiation total hardness in the control was 13°dH (232 mg/l as CaCO3).
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod /8 hours darkness daily.
EFFECT MEASURED
After 24 h and 48 h, immobilised daphnids were counted. All daphnids not able to swim within 15 s after gentle agitation of test vessel were considered to be immobilised. If present, behavioural changes of daphnids were recorded at 24 and 48 h after starting the test.
The NOEC was established based on the highest concentration at which the immobilisation is not higher than the allowed control immobilisation (± 10 % immobilisation).
MEASUREMENTS
Test temperature and pH-value as well as oxygen saturation rate of test solutions were measured at all concentrations at t = 0 h from fresh test solutions and after 24 h and 48 h from aged test solutions in one separate replicate per test item concentration without test organisms. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- No immobilisation was recorded over the observation period of 48 hours.
MEASURED CONCENTRATIONS
The fresh measured concentration of test item at 100 mg/l was 97 % of nominal and the aged measured concentration was 95 %. - Results with reference substance (positive control):
- EC50 (24h): between 1.0 and 2.0 mg/l.
- Reported statistics and error estimates:
- No statistical evaluation was indicated since no immobilisation occurred at the limit test concentration of 100 mg/l.
- Validity criteria fulfilled:
- yes
- Remarks:
- in the control not more than 10 % of animals were immobilised and the dissolved oxygen concentration was ≥ 3 mg/l at the end of the test in control and test vessels
- Conclusions:
- EC50 (48 h) > 100 mg/l (nominal)
- Executive summary:
Test item short-term toxicity to aquatic invertebrates was assessed using Daphnia magna, according to the OECD guideline 202. Twenty organisms per test concentration (4 replicates of 5 animals each) were used. The duration of the test was 48 hours. A static limit test with concentrations of 0 and 100 mg/l was performed. Analytical samples taken at 0 hours (initial value) from fresh test solutions and after 48 hours from aged test solutions were analysed from control and 100 mg/l. The initial measured content at 100 mg/l was 97 % of nominal and the aged measured content was 95 % of nominal. Therefore the toxicological endpoints were evaluated using nominal concentrations. After the 48 hours exposure period, no immobilisation was recorded at 100 mg/l, thus this concentration can be considered as a No Observed Effect Concentration.
Conclusion
EC50 (48 h) > 100 mg/l (nominal)
Reference
IMMOBILIZATION
Overall of results
Concentration | N. immobilized daphnids | % of immobilization | ||
24 hrs | 48 hrs | 24 hrs | 48 hrs | |
Control | 0 | 0 | 0 | 0 |
Control | 1* | 1* | 0 | 0 |
Control | 0 | 0 | 0 | 0 |
Control | 0 | 0 | 0 | 0 |
Test item 100 mg/l | 0 | 0 | 0 | 0 |
Test item 100 mg/l | 0 | 0 | 0 | 0 |
Test item 100 mg/l | 0 | 0 | 0 | 0 |
Test item 100 mg/l | 0 | 0 | 0 | 0 |
*died by accident
Reference substance
Concentration | N. immobilized daphnids | % of immobilization | ||
24 hrs | 48 hrs | 24 hrs | 48 hrs | |
K2Cr2O7 1.0 mg/l | 0 | 1 | 0 | 10 |
K2Cr2O7 1.0 mg/l | 1 | 1 | 10 | 10 |
K2Cr2O7 1.0 mg/l | 0 | 0 | 0 | 0 |
K2Cr2O7 1.0 mg/l | 0 | 0 | 0 | 0 |
K2Cr2O7 2.0 mg/l | 5 | 5 | 100 | 100 |
K2Cr2O7 2.0 mg/l | 4 | 5 | 80 | 100 |
K2Cr2O7 2.0 mg/l | 5 | 5 | 100 | 100 |
K2Cr2O7 2.0 mg/l | 4 | 5 | 80 | 100 |
MEASURED CONCENTRATIONS
Sampling (h) | Test item found | ||
mg/l | % of nominal | ||
Control | 0 fresh | n.d. | - |
48 aged | n.d. | - | |
Test item | 0 fresh | 97 | 97 |
48 aged | 95 | 95 |
Description of key information
EC50 (48 h) > 100 mg/l (nominal)
Key value for chemical safety assessment
Additional information
There are no information about the short-term toxicity potential to aquatic invertebrates of Acid Red 057, thus the available data on structural analogous Similar Substance 01 has been taken into account. The read across approach can be considered as reliable and suitable for the purpose; details and explanations are included in the report attached to the IUCLID section 13.
Similar Substance 01 was assessed using Daphnia magna, according to the OECD guideline 202. A static limit test with concentrations of 0 and 100 mg/l was performed. Analytical samples taken at 0 hours (initial value) from fresh test solutions and after 48 hours from aged test solutions were analysed from control and 100 mg/l: the initial measured content at 100 mg/l was 97 % of nominal and the aged measured content was 95 % of nominal. Therefore, the toxicological endpoints were evaluated using nominal concentrations. After the 48 hours exposure period, no immobilisation was recorded at 100 mg/l.
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