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EC number: 240-185-7 | CAS number: 16044-24-9
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- Sep 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- Dimethyl[4-[4-(dimethylamino)-α-phenylbenzylidene]-2,5-cyclohexadien-1-ylidene]ammonium hydrogen sulphate
- EC Number:
- 240-185-7
- EC Name:
- Dimethyl[4-[4-(dimethylamino)-α-phenylbenzylidene]-2,5-cyclohexadien-1-ylidene]ammonium hydrogen sulphate
- Cas Number:
- 16044-24-9
- Molecular formula:
- C23H25N2.HO4S
- IUPAC Name:
- N-(4-{[4-(dimethylamino)phenyl](phenyl)methylene}cyclohexa-2,5-dien-1-ylidene)-N-methylmethanaminium hydrogen sulfate
- Test material form:
- liquid
- Details on test material:
- Purity: 50% w/w dye
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- other: colour elimination
- Details on study design:
- Negative control: 1 replicate
Positive control: 1 replicate
Test substance: 1 replicate
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 28 d
- Parameter:
- other: % colour elimination
- Value:
- 88
- Sampling time:
- 28 d
- Details on results:
- The substance mainly adsorbs to activated sludgle particles. The DOC removal is therefore not considered relevant.
BOD5 / COD results
- Results with reference substance:
- 100% removal after 4 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The substance reached an elimination of ca. 98% after 28 d. However, the substance was found to adsorb to activated sludge particles based on the colourimetric measurements. The DOC removal is therefore not considered relevant. The substance is not found to be inherently biodegradable in this OECD 302B test.
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