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EC number: 231-203-4 | CAS number: 7446-26-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- A chromosomal aberration assay was conducted in rats to evaluate the genotoxic potential of zinc sulphate. The study utilizes examination of bone marrow cells arrested in C-metaphase from rats exposed to the test compound as compared to positve and negative control animals.
- GLP compliance:
- no
- Type of assay:
- mammalian bone marrow chromosome aberration test
Test material
- Reference substance name:
- Zinc sulphate
- EC Number:
- 231-793-3
- EC Name:
- Zinc sulphate
- Cas Number:
- 7733-02-0
- Molecular formula:
- H2O4S.Zn
- IUPAC Name:
- zinc sulfate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Flow Laboratories
- Age at study initiation: 12 weeks old
- Weight at study initiation: 280 to 350 g
- Assigned to test groups randomly: not specified
- Fasting period before study:
- Housing: one to five per cage
- Diet: not specified
- Water: not specified
- Acclimation period: 4 - 11 days
ENVIRONMENTAL CONDITIONS
not specified
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: 0.85% saline
- Duration of treatment / exposure:
- acute study: once
subacute study: five days - Frequency of treatment:
- subacute: daily
- Post exposure period:
- acute study: 6, 24 and 48 hours
subacute study: 6 hours after last dose
Doses / concentrationsopen allclose all
- Dose / conc.:
- 2.75 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 27.5 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 275 mg/kg bw/day (actual dose received)
- No. of animals per sex per dose:
- 5 animals per treatment group
3 animals per control group - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- triethylenemelamine
- Route of administration: one treatment intraperitoneally, after 48 hours cells were prepared for analysis
- Doses / concentrations: 0.3 mg/kg bw
Examinations
- Tissues and cell types examined:
- bone marrow cells
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: preliminary toxicity test: LD5 was used as highest dose, 1/10 of LD5 was used as intermediate dose and 1/100 of LD5 was used as low dose
DETAILS OF SLIDE PREPARATION: Four hours after the last compound administration, and two hours prior to killing, each animal was given 4 mg/kg of colcemid intraperitoneally in order to arrest the bone marrow cells in C-mitosis. Animals were killed by using CO2, and the adhering muscle and epiphysis of one femur were removed. The marrow "plug" was removed with a tuberculin syringe and an 18 gauge needle, aspirated into 5 mL of Hanks' balanced salt solution (BSS) in a test tube and capped. The specimens were centrifuged at 1500 rpm in a table-top centrifuge for 5 minutes, decanted, and 2 mL of hypotonic 0.5% KCl solution was added with gentle agitation to resuspend the cells. The specimens were then placed in a 37°C water bath for 20 minutes in order to swell the cells. Following centrifugation for 5 minutes at 1500 rpm, the supernatant was decanted and 2 mL of fixative (3:1 absolute methanol:glacial acetic acid) was added. The cells were resuspended in the fixative with gentle agitation, capped, and placed at 4°C for 30 minutes. The specimens were again centrifuged, decanted, 2 mL of prepared fixative was added, and the cells were resuspended and placed at 4°C overnight. The following day the specimens were again centrifuged, decanted and 0.3 - 0.6 mL of freshly prepared fixative was added to obtain a suitable density. The cells were resuspended and 2 - 3 drops of the suspension were allowed to drop onto a clean, dry slide held at 15°C from the horizontal. As the suspension flowed to the edge of the slide, it was ignited by an alcohol burner and allowed to flame. Following ignition, the slides were allowed to dry at room temperature overnigt. Duplicate slides were prepared. The slides were stained using a 5% Giemsa solution for 20 minutes, rinsed in acetone, 1:1 acetone:xylene, and placed in fresh xylene for 30 minutes. The slides were then mounted using Permount and 24 x 50 mm coverglasses.
METHOD OF ANALYSIS: Suitable metaphase spreads that were countable were examined. The chromosomes of each cell were counted and only diploid cells were analysed. They were scored for chromatid gaps and breaks, chromosome gaps and breaks, reunions, cells with greater than ten aberrations, polyploidy, pulverisation, and any other chromosomal aberrations which were observed. 50 metaphase spreads were scored per animal. Mitotic indices were obtained by counting at least 500 cells and the ratio of the number of cells in mitosis/the number of cells observed was expressed as the mitotic index. - Evaluation criteria:
- not reported
- Statistics:
- not reported
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 1: acute study: metaphase summary sheet |
|||||||||
Compound |
Dose (mg/kg bw) |
Time* |
# of animals |
# of cells |
Mitotoic index %*** |
% cells with breaks |
% cells with reunion |
% cells other aberr.** |
% cells with aberr. |
Negative control |
saline |
6 |
3 |
150 |
4 |
0 |
0 |
0 |
0 |
24 |
3 |
150 |
5 |
0 |
0 |
0 |
0 |
||
48 |
3 |
150 |
7 |
0 |
0 |
0 |
0 |
||
Low level |
2.75 |
6 |
5 |
250 |
5 |
0 |
0 |
0 |
0 |
24 |
5 |
200 |
4 |
0 |
0 |
0 |
0 |
||
48 |
5 |
250 |
3 |
0 |
0 |
0 |
0 |
||
Intermediate level |
27.5 |
6 |
5 |
250 |
9 |
0 |
0 |
0 |
0 |
24 |
5 |
250 |
4 |
0 |
0 |
0 |
0 |
||
48 |
5 |
250 |
7 |
0 |
0 |
0 |
0 |
||
LD5 |
275 |
6 |
5 |
250 |
4 |
0 |
0 |
0 |
0 |
24 |
5 |
250 |
4 |
0 |
0 |
0 |
0 |
||
48 |
5 |
250 |
6 |
0 |
0 |
0 |
0 |
||
Positive control TEM |
0.3 |
48 |
5 |
250 |
4 |
0.4 |
27 |
14(a) |
41 |
* time of kill after injection (hours) ** cells that have polyploidy (P), pulverisation (pp), fragments (f) or greater than 10 aberrations (a). *** % of cells in mitosis: 500 cells observed/animal. |
Table 2: subacute study: metaphase summary sheet |
||||||||
Compound |
Dose* (mg/kg bw) |
# of animals |
# of cells |
Mitotoic index %*** |
% cells with breaks |
% cells with reunion |
% cells other aberr.** |
% cells with aberr. |
Negative control |
saline |
3 |
150 |
8 |
0 |
0 |
0 |
0 |
Low level |
2.75 |
5 |
250 |
5 |
0 |
0 |
0 |
0 |
Intermediate level |
27.5 |
5 |
250 |
8 |
0 |
0 |
0 |
0 |
LD5 |
275 |
5 |
250 |
6 |
0 |
0 |
0 |
0 |
* Dosage 1x/day x 5 days ** cells that have polyploidy (P), pulverisation (pp), fragments (f) or greater than 10 aberrations (a). *** % of cells in mitosis: 500 cells observed/animal. |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
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