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EC number: 221-203-2 | CAS number: 3030-80-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- (Q)SAR
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
- Justification for type of information:
- Please refer to attached QMRF and QPRF Documents
- Principles of method if other than guideline:
- Calculation of toxicity in Algae. Software used: ECOSAR 1.11 (EPISUITE) for chemical class "Imidazols"
- GLP compliance:
- no
- Specific details on test material used for the study:
- SMILES: c12nc(S)nc1cccc2
- Analytical monitoring:
- not required
- Test organisms (species):
- other: Algae
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.214 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: EC50 (acute value, QSAR)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.205 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: ChV (chronic value, QSAR)
- Conclusions:
- Using ECOSAR v1.11 the short- (EC50) and long-term (NOEC) toxicity values for algae were calculated to be 0.214 mg/L and 0.205 mg/L, respectively. The substance is within the applicability domain of the model.
- Executive summary:
Using ECOSAR v1.11 the short- (EC50) and long-term (NOEC) toxicity values for algae were calculated to be 0.214 mg/L and 0.205 mg/L, respectively. The substance is within the applicability domain of the model.
The adequacy of a prediction depends on the following conditions:
a) the (Q)SAR model is scientifically valid: the scientific validity is established according to the OECD principles for (Q)SAR validation;
b) the (Q)SAR model is applicable to the query chemical: a (Q)SAR is applicable if the query chemical falls within the defined applicability domain of the model;
c) the (Q)SAR result is reliable: a valid (Q)SAR that is applied to a chemical falling within its applicability domain provides a reliable result;
d) the (Q)SAR model is relevant for the regulatory purpose.
For assessment and justification of these 4 requirements the QMRF and QPRF files were developed and attached to this study record.
Description of the prediction Model
The prediction model was descripted using the harmonised template for summarising and reporting key information on (Q)SAR models. For more details please refer to the attached QSAR Model Reporting Format (QMRF) file.
Assessment of estimation domain
The assessment of the estimation domain was documented in the QSAR Prediction Reporting Format file (QPRF). Please refer to the attached document for the details of the prediction and the assessment of the estimation domain.
Reference
Description of key information
In this 72-h algal growth inhibition test with Raphidocelis subcapitata, the obtained results showed that the test item Zinc di(benzimidazol-2-yl) disulphide had significant toxic effects on the growth of Raphidocelis subcapitata. The 72-h EC10, EC20, EC50 for growth rate were determined to be 0.008, 0.014 and 0.034 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.034 mg/L
- EC10 or NOEC for freshwater algae:
- 0.008 mg/L
Additional information
A Growth Inhibition Test according to OECD guideline 201 was conducted to determine the effect of the test item Zinc di(benzimidazol-2-yl) disulphide on the growth of the unicellular green algae species Raphidocelis subcapitata (formerly known as Pseudokirchneriella subcapitata) (TOXICOOP, 2018). Exponentially growing cultures of Raphidocelis subcapitata were exposed to nominal test item concentrations of 0.014, 0.041, 0.12, 0.37, 1.11, 3.33 and 10.0 mg/L. The analytically measured test item concentrations were not within the range of ± 20 % of the nominal over the test period of 72 hours. Therefore, exposure concentrations were calculated as the geometric mean of the measured concentrations. Corresponding calculated geometric mean measured concentrations were: 0.003, 0.005, 0.01, 0.06, 0.54, 2.26 and 7.27 mg/L. Concurrent control ran parallel in the test. The algal growth in relation to the untreated control culture was determined over a fixed test period of 72 hours and thus, over several algal generations. The 72-h EC10, EC20, EC50 for growth rate were determined to be 0.008, 0.014 and 0.034 mg/L, respectively. The 72-h LOEC values based on growth rate was determined to be 0.01 mg/L. The 72-h NOEC for growth rate was determined to be 0.005 mg/L. All validity criteria were met. The results are based on measured geometric mean test item concentrations.
A QSAR calculation using ECOSAR v1.11 determined short- (EC50) and long-term (NOEC) toxicity values for algae were calculated to be 0.214 mg/L and 0.205 mg/L, respectively.
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