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EC number: 228-800-7 | CAS number: 6359-50-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-05-08 to 1990-05-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
- EC Number:
- 228-800-7
- EC Name:
- 2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
- Cas Number:
- 6359-50-8
- Molecular formula:
- C22H25N2.Cl
- IUPAC Name:
- 2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
- Test material form:
- solid: particulate/powder
- Details on test material:
- Basic Yellow 21
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- Bor: WISW (SPF Cpb)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: male: 8 weeks, female: 10 weeks
- Weight at study initiation: average male: 177g female: 171g
- Fasting period before study: 16h before and 4h after application fasting
- Housing: Makrolon cage Type III, 5 animals per cage
- Diet (e.g. ad libitum): Altromin 1324 ad libitum
- Water (e.g. ad libitum): mains water ad libitum
- Acclimation period: at least 4 days acclimation
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2°C
- Humidity (%): 50 +/- 10%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12h/12h
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- DOSAGE PREPARATION: the test substanc was mixed in mains water at ambient temperature
VEHICLE
- Concentration in vehicle: 5, 10, 20 mg/mL
- Amount of vehicle (if gavage): 10 mL/kg bw
- Justification for choice of vehicle: good water solubility
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg bw - Doses:
- 50, 100 and 200 mg/kg bw based on preliminary study
- No. of animals per sex per dose:
- 5 per sex per dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: day of application: multiple observations; during 14 days observation: twice per working day, once on weekend and holiday; weekly weighing
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- LD50 calculation based on "moving averages" according to Spearman and Kärber.
In case of dose levels with 0 and 100% mortality determination of geometric mean as "approximative" LD50
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 162 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 106 - 250
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 140 mg/kg bw
- Based on:
- act. ingr.
- Mortality:
- one and three rats/sex died at 100 and 200 mg/kg bw, respectively - see table below
- Clinical signs:
- 100 and 200 mg/kg bw: bad general condition, sedation and prone position
100 mg/kg bw: piloerection in one male and one female animal
clinical signs occured 15 minutes after application and disappeared at the latest after 2 days
no clinical signs in the lowest dose of 50 mg/kg bw - Body weight:
- not affected
- Gross pathology:
- died animals: affected stomach mucosa, fundus of the stomach and intestine reddened and tumid
survived animals: no findings
Any other information on results incl. tables
dose [mg/kg bw] |
animals treated/ animals died | time of death | mortality [%] |
male | |||
50.0 | 0/5 | - | 0 |
100.0 | 1/5 | 2d | 20 |
200.0 | 3/5 | 1-4h | 60 |
female | |||
50.0 | 0/5 | - | 0 |
100.0 | 1/5 | 1h | 20 |
200.0 | 3/5 | 1-4h | 60 |
dose [mg/kg bw] |
average body weight [g] | ||
male | |||
week 0 | week 1 | week 2 | |
50.0 | 183 | 225 | 253 |
100.0 | 174 | 222 | 250 |
200.0 | 175 | 218 | 242 |
female | |||
week 0 | week 1 | week 2 | |
50.0 | 177 | 189 | 192 |
100.0 | 164 | 184 | 193 |
200.0 | 173 | 196 | 202 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- The LD50 (oral, gavage) of the test substance for acute toxicity to male and female rats was calculated to be 162 mg/kg bw. The substance is classified as Category 3.
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