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EC number: 224-736-9 | CAS number: 4468-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- LCE11031, Batch 1106900005 (zinc gluconate)
- Details on sampling:
- At the beginning and the end ot test, aliquot (approximately 20 ml.) was taken in each group containing the test item and in the control.
These aliquots were frozen and transferred to ICP unit to be analyzed for Zinc contents determination. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Stock solutions were kept at 4 ± 2°C until they were used for the study. Stock solutions were used because they were not cloudy (without any precipitation).
on March 29th , 2011, April 4th, 2011 and April 11th, 2011, 250 mL of stock solution 1, 2, 3, and 4 were mixed with 800 rnL demineralised water and completed to 10L.
Test medium was kept less than I week at 4 ± 2°C
Test medium was used on April 1st, 5th and 12th, 2011. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia
- Strain: Daphnia magna (prescribed by test guideline)
- Source: SGS breeding lab
- Age of parental stock: Daphnids between 6 and 24 hours old and produced from parthenogenesis reproducing brood female population.
Every day, daphnids were filtered on the following sieves (200 pm, 560pm and 800prn). We used for the test, Daphnids which pass through 800 µm sieve and remain on 560 µm sieve.
ACCLIMATION
no detail provided in the study report - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Standard duration
- Hardness:
- not specified
- Test temperature:
- 20°C ± 2
- pH:
- 7.8 ± 0.2
- Dissolved oxygen:
- > 7mg/L (90%)
- Nominal and measured concentrations:
- 0mg/L (control) ; 2.0196 mg/L ; 4.0392 mg/L ; 8.0784 mg/L ; 16.1568 mg/L ; 32.3136 mg/L
- Details on test conditions:
- TEST SYSTEM
- Aeration: During the preparation, test medium was ventilated until a value close to saturation in air.
OTHER TEST CONDITIONS
- Light intensity: Test carried out in the dark
PARAMETERS MEASURED:
The pH was measured in the control and all test concentrations, in the fresh solutions and in the old solutions.
The dissolved oxygen concentration was measured in control and in the lowest concentration that produced the maximum effect.
RANGE-FINDING STUDY
- Test concentrations: 0.01 - 0.1 - 1 - 10 - 100 mg/L (divided into 4 replicates including 1 control replicate (medium only) and 5 daphnids introduced in each replicate)
- Results used to determine the conditions for the definitive study: 50% of mortality (average between replicates) is observed at 10.9 mg/L - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 22.8 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).
- Reported statistics and error estimates:
- Confidence limits (95%) 20.4-25.3 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).
- Executive summary:
The study is an acute toxicity test performed on Daphnia Magna according to the OECD 202 guidline and to GLP. This study is considered reliable and it is used for assessment. The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).
Reference
LCE11031 (mg/L) |
Mortality Daphnids at 48h |
Mortality (%) |
|||
Replicate A |
Replicate B |
Replicate C |
Replicate D |
||
Control |
0 |
0 |
0 |
0 |
0 |
2.0196 |
0 |
0 |
0 |
0 |
0 |
4.0392 |
0 |
0 |
0 |
0 |
0 |
8.0784 |
0 |
0 |
0 |
0 |
0 |
16.1568 |
1 |
2 |
0 |
2 |
25 |
32.3136 |
3 |
4 |
3 |
5 |
75 |
5 mobile dphnids per replicate at t=0
Description of key information
The study is an acute toxicity test performed on Daphnia Magna according to the OECD 202 guidline and to GLP. This study is considered reliable and it is used for assessment. The EC50-48h was determined on Daphnia Magna at 22.8mg/L (Confidence limits (95%) 20.4-25.3 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 22.8 mg/L
Additional information
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