N-cyclohexylcyclohexanamine 2-[1-[[[(1R)-1-[3-[(1E)-2-(7-chloroquinolin-2-yl)ethenyl]phenyl]-3-[2-(2-hydroxypropan-2-yl)phenyl]propyl]sulfanyl]methyl]cyclopropyl]acetate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

other: OECD SIDS

Title:

Unnamed

Year:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: other guideline: OECD Guideline 202, part 2 "Daphnia sp., Reproduction Test" and the Draft Test Guideline 211 (April, 1997, OECD)

Principles of method if other than guideline:

Method: other: OECD Guideline 202, part 2 "Daphnia sp., Reproduction Test" and the Draft Test Guideline 211 (April, 1997, OECD)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dicyclohexylamine

EC Number:

202-980-7

EC Name:

Dicyclohexylamine

Cas Number:

101-83-7

IUPAC Name:

N-cyclohexylcyclohexanamine

Test material form:

liquid

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
IUCLID4 Test substance: other TS: Dicyclohexylamine, CAS:100-74-3, Wako Pure Chemical Industries, Ltd., Lot. No.: WtK5069, purity = 99.9 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnids were cultured under the incubation conditions (see below) and fed with Chlorella vulgaris. Incubation at 20 +/- 1 °C during 16 h/8 h light/darkness regime, every 2-3 d exchange of incubation medium

Study design

Test type:

semi-static

Water media type:

not specified

Test conditions

Hardness:

- total hardness 55.2 mg/l CaCO3,   containing (all in mg/l) COD <1, P <0.01, total Hg <0.0005, Cu <0.01,  Cd <0.001, Zn <0.01, Pb <0.005, Al 0.03, Ni <0.02, total Cr <0.02, soluble Mn <0.005, Sn <0.03, soluble Fe <0.03, CN- <0.01, free Cl <0.01, Br- <0.5, F- 0.45, S2- <0.01, NH4+ <0.01, As <0.001, Se <0.001, Na 53, K 4.9, Ca 16, Mg 3.3, total chlorinated pesticides <0.00002, total organophosphorus pesticides <0.001, PCB <0.0005. Total hardness was determined in the incubation media several times during the assays and was found to be 60-80 mg/l

Test temperature:

Incubation at 20 +/- 1 °C

pH:

Dilution water, pH 8.1, pH was monitored at start and at the end of the incubations. pH was 7.8-8.2 during incubation

Dissolved oxygen:

- Oxygen concentration was 7.4-8.6 mg/l during incubation

Details on test conditions:

Incubation at 20 +/- 1 °C during 16 h/8 h light/darkness regime, every 2-3 d exchange of incubation medium

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.016 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.049 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.14 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

1.3 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

mortality

Remarks on result:

other: (95 % confidence limits: 0.54-10 mg/l, Probit)

Reported statistics and error estimates:

Statistical method: For LC50 Probit and EcoTox-Statics Release 1.1, for EC50 Logistic and EcoTox-Statics Release 1.1, and for NOEC and LOEC Non-parametric Dunnett and EcoTox-Statics Release 1.1.

Any other information on results incl. tables

21 d-LC50 for mortality of parent Daphnias was 1.3 mg/l (95
% confidence limits: 0.54-10 mg/l, Probit). In detail, the
cumulative mortality of parental Daphnias (%) was:
        7d        14d        21d
Control        0        0        0
0.016        0        0        0
0.049        0        0        10
0.23        0        20        20
0.80        0        10        30
2.7        10        20        70
21 d-EC50 for inhibition of reproduction was 0.14 mg/l
(Logistic). In detail, the mean cumulative numbers of
juveniles produced per adult were:
        7d        14d        21d
Control        2.2        46.8        114.9
0.016        4.4        36.6        135.2
0.049        3.6        37.3        91.6
0.23        2.4        14.8        44.4
0.80        1.1        2.3        13.3
2.7        0.0        0.0        0.0
Reproduction was slightly increased at 0.016 mg/l, but
completely inhibited at 2.7 mg/l:
Conc.        Control        0.016        0.049        0.23        0.80        2.7
Mean        114.9        135.2        91.6        44.4        13.3        0.0
S.D.        35.4        43.2        37.8        11.3        13.2        0.0
Inhibition (%)        -18        20        61        88        100
The time (d) to first brood production was not influenced by
cyclohexylamine up to 0.049 mg/l:
Vessel  Control        0.016        0.049        0.23        0.80        2.7
1        7        7        7        7        8        -
2        7        7        7        9        8        -
3        7        7        7        7        8        -
4        7        7        7        8        8        -
5        7        7        7        7        8        -
6        7        7        7        8        8        -
7        8        7        7        8        8        >21
8        8        7        8        8        8        >21
9        7        8        7        7        7        >21
10        7        7        7        7        8        -
Mean        7.2        7.1        7.1        7.6        7.9        >21

Effect Data(Reproduction): 
LC50 (21d) = 1.3 (0.54-10) mg/ml  (parental mortality) (mc)
EC50 (21d) = 0.14 mg/L (Reproduction) (mc) 
NOEC (21d) = 0.016 mg/L (Reproduction) (mc)
LOEC (21d) = 0.049 mg/L (Reproduction) (mc)
mc: based on time-weighted mean of measured concentrations

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results were reported for daphnia magna:

Effect Data (Reproduction):  LC50 (21d) = 1.3 mg/ml  (parental mortality); EC50 (21d) = 0.14 mg/L (Reproduction); NOEC (21d) = 0.016 mg/L (Reproduction) and LOEC (21d) = 0.049 mg/L (Reproduction)