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EC number: 210-782-7 | CAS number: 623-25-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 February 2018 to 23 March 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- Commission Regulation (EU) 2017/735 of 14 February 2017 amending, for the purpose of its adaptation to technical progress, the Annex to Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH), Method C.4-E “Closed Bottle Test"
- Deviations:
- yes
- Remarks:
- See "Any other information" for details
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- OECD Guideline for Testing of Chemicals No. 301 D: "Ready Biodegradability: Closed Bottle Test", adopted July 17, 1992
- Deviations:
- yes
- Remarks:
- See "Any other information" for details
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Version / remarks:
- EPA Guideline 712-C-98-076: OPPTS 835.3110, "Ready Biodegradability", January 1998
- Deviations:
- yes
- Remarks:
- See "Any other information" for details
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- No further details specified in the study report.
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The inoculum: Secondary effluent, microorganisms from a domestic waste water treatment plant.
Origin: The secondary effluent was supplied from the sewage plant for domestic sewage in Veszprém, Hungary.
Conditioning: The secondary effluent used for this study was allowed to settle approximately for an hour, then the decanted effluent was aerated until use (not later than an hour). - Duration of test (contact time):
- 28 d
- Initial conc.:
- 11.6 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST UNITS
Type and Size: BOD bottles (300 ml) with special neck and glass stoppers.
Identification: Each test flask was uniquely identified with study code, test group, days of measurement and replicate number.
TEST CONDITIONS
The test was carried out in an incubator and controlled environment room (during the formulation and oxygen measuring) at a temperature of 22 ± 2°C according to guideline.
The test flasks were placed into an incubator and kept at 20.4 - 23.3°C, in the dark. The temperature was measured on weekdays during the experiment.
The oxygen concentration of test water was 9.0 mg/L at the start of the test.
The pH value of the test water was checked prior start of the experiment. The pH of the test water was 7.22.
The test conditions were measured with suitable instruments and documented in the raw data.
PREPARATION OF THE TEST SOLUTIONS
The respective amount of α, α’-Dichloro-p-xylene was weighed in directly to reach the required test item concentration of 3.4 mg/L.
The chosen test item concentration was based on theoretical oxygen demand (ThOD) of 1.74 mg O2/mg test item (calculated according to equation given in the guidelines) and on the performed 14-d preliminary test.
The components were applied in the amounts/volumes in the test flasks:
1.) Test Item (flasks 1a and 1b)
Based on the theoretical oxygen demand (ThOD) of 1.74 mg O2/mg test item, 11.6 mg of α, α’-Dichloro-p-xylene was thoroughly mixed into 3.40 litres of aqueous test medium (corresponding to 3.4 mg/L test item, with a ThOD of about 5.916 mg O2/L).
2.) Procedure Control: Sodium benzoate (flasks 2a and 2b)
Based on the theoretical oxygen demand (ThOD) of Sodium benzoate (1.67 mg O2 per mg; details on calculation are given in the guidelines), stock solution* corresponding to 12.168 mg of Sodium benzoate was mixed into 3.38 litres of aqueous test medium (corresponding to 3.6 mg/L reference item, respectively a ThOD of about 6.012 mg O2/L).
* The concentration of the stock solution was: 360 mg/L.
3.) Inoculum Control (flasks 3a and 3b)
Only filtered inoculum was added to 3.38 litres of aqueous test medium.
4.) Toxicity Control (flasks 4a and 4b)
The Test item (11.6 mg) and the reference item stock solution* (34.0 mL) were mixed into 3.40 litres of aqueous test medium corresponding to 3.4 mg/L test item (ThOD of 5.916 mg O2/L) and 3.6 mg/L reference item (ThOD of 6.012 mg O2/L).
* The concentration of the reference item stock solution was: 360 mg/L.
Microbial inoculum (0.5 mL per litre) was added to each preparation bottle.
COURSE OF THE TEST
Preparation of Test Flasks
Sufficient number of BOD flasks was cleaned with 5 - 10 mL of a wash liquid (2.5 g iodine and 12.5 g potassium iodide per litre of 1 % w/v sulphuric acid) by shaking well to coat the bottle walls. After allowing to stand for 15 minutes, the wash liquid was poured off, and the bottles were thoroughly rinsed with tap water and deionised water.
Then, the previously described test solutions were filled into the bottles bubble-free until the bottles were completely filled. Then they were tightly closed with glass stoppers.
The Test Bottles
The number of test bottles was the follow:
10 bottles containing the test item and inoculum
-10 bottles containing the reference item and inoculum (procedure control)
-10 bottles containing only inoculum (inoculum control)
-10 bottles containing the test item, reference item and inoculum (toxicity control)
MEASUREMENTS
Measurement of Oxygen
The incubation period of the closed bottle test was 28 days.
The oxygen concentrations were measured with an oxygen meter with a stirring O2 electrode. Oxygen measurements were performed in duplicate on days 0, 7, 14, 21 and 28.
Measurement of Temperature
Temperature was measured continuously and registered on weekdays. - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7.6
- Sampling time:
- 28 d
- Details on results:
- Under the test conditions the percentage biodegradation of α, α’-Dichloro-p-xylene reached a mean of 7.6 % after 28 days based on the ThOD of the test item.
In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 39.0 % biodegradation was noted within 14 days and 42.0% biodegradation after 28 days of incubation. - Results with reference substance:
- The reference item Sodium benzoate was sufficiently degraded to a mean of 76.7 % after 14 days, and to a mean of 82.5 % after 28 days of incubation, based on ThOD, thus confirming the suitability of the used inoculum.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item α, α’-Dichloro-p-xylene was investigated for its ready biodegradability in a Closed Bottle Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The test system was a microbial inoculum of good quality, collected from a sewage plant for domestic sewage. The sludge was allowed to settle and then aerated until use.
Under the test conditions the percentage biodegradation of α, α’-Dichloro-p-xylene reached a mean of 7.6 % after 28 days based on the ThOD of the test item. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThOD. Therefore the test item is considered not readily biodegradable.
The reference item Sodium benzoate was sufficiently degraded to a mean of 76.7 % after 14 days, and to a mean of 82.5 % after 28 days of incubation, based on ThOD, thus confirming the suitability of the used inoculum.
In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 39.0 % biodegradation was noted within 14 days and 42.0% biodegradation after 28 days of incubation.
According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 3.4 mg/L on the secondary effluent microorganisms because degradation was >25 % within 14 days.
The test item was not considered to have significant inhibitory effects on the secondary effluent microorganisms.
Based on the results of this study, the test item is considered not readily biodegradable. - Executive summary:
The test item α,α’-Dichloro-p-xylene was investigated for its ready biodegradability in a Closed Bottle Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The test system was a microbial inoculum of good quality, collected from a sewage plant for domestic sewage. The sludge was allowed to settle and then aerated until use.
Under the test conditions the percentage biodegradation of α, α’-Dichloro-p-xylene reached a mean of 7.6 % after 28 days based on the ThOD of the test item. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThOD. Therefore, the test item is considered not readily biodegradable.
The reference item Sodium benzoate was sufficiently degraded to a mean of 76.7 % after 14 days, and to a mean of 82.5 % after 28 days of incubation, based on ThOD, thus confirming the suitability of the used inoculum.
In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 39.0 % biodegradation was noted within 14 days and 42.0% biodegradation after 28 days of incubation.
According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 3.4 mg/L on the secondary effluent microorganisms because degradation was >25 % within 14 days.
The test item was not considered to have significant inhibitory effects on the secondary effluent microorganisms.
Based on the results of this study, the test item is considered not readily biodegradable.
Reference
Dissolved Oxygen Concentrations at Different Time Intervals during the Exposure Period of 28 Days
Treatment |
Concentration [mg/L] |
Flask No. |
mg O2/L after n days of exposure |
||||
0 |
7 |
14 |
21 |
28 |
|||
Test item |
3.4 |
1a 1b mean |
8.6 8.5 8.55 |
7.7 7.9 7.80 |
7.3 7.4 7.35 |
7.1 7.2 7.15 |
6.8 6.8 6.80 |
Reference item |
3.6 |
2a 2b mean |
8.8 8.7 8.75 |
4.4 4.4 4.40 |
3.2 3.4 3.30 |
2.8 2.9 2.85 |
2.4 2.6 2.50 |
Inoculum control |
- |
3a 3b mean |
8.8 8.8 8.80 |
8.6 8.5 8.55 |
7.9 8.0 7.95 |
7.7 7.8 7.75 |
7.6 7.4 7.50 |
Toxicity control |
Test item: 3.4 Reference item: 3.36 |
4a 4b mean |
8.7 8.6 8.65 |
4.3 4.3 4.30 |
3.2 3.1 3.15 |
2.6 2.6 2.60 |
2.3 2.4 2.35 |
Oxygen Depletion at Different Time Intervals during the Exposure Period of 28 Days
Treatment |
Concentration [mg/L] |
Flask No. |
mg O2/L after n days of exposure |
|||
7 |
14 |
21 |
28 |
|||
Test item |
3.4 |
1b 1b |
0.65 0.35 |
0.45 0.25 |
0.45 0.25 |
0.50 0.40 |
Reference item |
3.6 |
2a 2b |
4.15 4.05 |
4.75 4.45 |
4.95 4.75 |
5.10 4.80 |
Toxicity control |
Test item: 3.4 Reference item: 3.6 |
4a 4b |
4.15 4.05 |
4.65 4.65 |
5.05 4.95 |
5.10 4.90 |
Oxygen depletion: (mt0 – mtx) – (mb0 – mbx), where:
mt0: oxygen concentration (mg/L) of test group on day 0
mtx: oxygen concentration (mg/L) of test group on day x
mb0: oxygen concentration (mg/L) of inoculum blank on day 0
mbx: oxygen concentration (mg/L) of inoculum blank on day x
BOD at Different Time Intervals during the Exposure Period of 28 Days
Treatment |
Concentration [mg/L] |
Flask No. |
mg O2/L after n days of exposure |
|||
7 |
14 |
21 |
28 |
|||
Test item |
3.4 |
1b 1b |
0.19 0.10 |
0.13 0.07 |
0.13 0.07 |
0.15 0.12 |
Reference item |
3.6 |
2a 2b |
1.15 1.13 |
1.32 1.24 |
1.38 1.32 |
1.42 1.33 |
Toxicity control |
Test item: 3.4 Reference item: 3.6 |
4a 4b |
0.59 0.58 |
0.66 0.66 |
0.72 0.71 |
0.73 0.70 |
BOD = (mg O2/L of T.i. and/or R.i. – mg O2/L if i.control) / mg Ti. And/or R.i./L in flask = mg O2/mg T.i. and/or R.i.
where:
T.i. = test item
R.i. = reference item
i.control = inoculum control
Percentage Biodegradation at Different Time Intervals during the Exposure Period of 28 Days
Treatment |
Concentration [mg/L] |
Flask No. |
mg O2/L after n days of exposure |
|||
7 |
14 |
21 |
28 |
|||
Test item |
3.4 |
1b 1b mean |
11.0 5.9 8.5 |
7.6 4.2 5.9 |
7.6 4.2 5.9 |
8.5 6.8 7.6 |
Reference item |
3.6 |
2a 2b mean |
69.2 67.5 68.3 |
79.2 74.2 76.7 |
82.5 79.2 80.8 |
85.0 80.0 82.5 |
Toxicity control |
Test item: 3.4 Reference item: 3.6 |
4a 4b mean |
34.8 34.0 34.4 |
39.0 39.0 39.0 |
42.4 41.6 42.0 |
42.8 41.1 42.0 |
Biodegradation% = [BOD (mg O2/mg T.i. or R.i.) / ThOD (mg O2/mg T.i.) or ThOD (mg O2/mg R.i.)] x 100
where:
T.i. = test item
R.i.= reference item
i.control = inoculum control
ThOD of test item = 1.74 mg O2/mg test item
ThOD of reference item = 1.67 mg O2/mg reference item
The biodegradation in the toxicity control was calculated according to the following formula:
(BOD (mg O2/mg T.i. and R.i.) / [ThOD (mg O2/mg T.i.) + ThOD (mg O2/mg R.i.)] * ½ ) x 100
Description of key information
The test item is considered not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The test item α,α’-Dichloro-p-xylene was investigated for its ready biodegradability in a Closed Bottle Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The test system was a microbial inoculum of good quality, collected from a sewage plant for domestic sewage. The sludge was allowed to settle and then aerated until use.
Under the test conditions the percentage biodegradation of α, α’-Dichloro-p-xylene reached a mean of 7.6 % after 28 days based on the ThOD of the test item. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThOD. Therefore, the test item is considered not readily biodegradable.
The reference item Sodium benzoate was sufficiently degraded to a mean of 76.7 % after 14 days, and to a mean of 82.5 % after 28 days of incubation, based on ThOD, thus confirming the suitability of the used inoculum.
In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 39.0 % biodegradation was noted within 14 days and 42.0% biodegradation after 28 days of incubation.
According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 3.4 mg/L on the secondary effluent microorganisms because degradation was >25 % within 14 days.
The test item was not considered to have significant inhibitory effects on the secondary effluent microorganisms.
Based on the results of this study, the test item is considered not readily biodegradable.
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