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EC number: 225-690-2 | CAS number: 5011-34-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 31 Aug. 2004 to 2 Sept. 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Trimetazidine
- EC Number:
- 225-690-2
- EC Name:
- Trimetazidine
- Cas Number:
- 5011-34-7
- Molecular formula:
- C14H22N2O3
- IUPAC Name:
- 1-[(2,3,4-trimethoxyphenyl)methyl]piperazine
- Test material form:
- liquid
- Details on test material:
- No details are provided in the study report
Constituent 1
- Specific details on test material used for the study:
- No analytical data are provided on the test material in the study report. Batch N°538271/0124
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of the stock solution:
To prepare the stock solution, the test item was dissolved or dispersed directly in M4 reconstituted water. The obtained preparation was agitated mechanically for up to 24 hours in an attempt to completely dissolve or disperse the test item.
Water: reconstituted (M4). The pH of this solution is 8.0 ± 0.5 and the sum of Ca and Mg ions in the solution is 2.5 mmol/L with a molar ratio of Ca to Mg ions of approximately 4:1, and of Na to K of 10:1.
Preparation of the test solutions:
Test solutions was prepared by further dilution of the stock solution with M4 or directly from the test item. A solution containing a maximum of 100 mg/L of the test item plus two other range-finding concentrations was prepared. Test vessels was filled directly from the test solution containers immediately after preparation.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species, clone: Daphnia magna STRAUS, clone 5.
Reason for this choice: most sensitive clone of the species recommended in OECD and EEC guidelines. Breeder: CIT.
Origin: Centre Technique du Bois et de l'Ameublement, 75012 Paris, France.
Age: between 6 and 24 hours.
Acclimation: none.
Allocation of the animals: animals was selected without preference and randomly assigned to test vessels.
Number of animals: 20 animals per concentration divided into four replicate groups of five animals.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Betwen 289mg/L and 306mg/L in the control flask.
- Test temperature:
- measured during the test but not specified in the report.
- pH:
- Between 8.32 and 8.95
- Dissolved oxygen:
- Between 8.8mg/L and 8.9mg/L.
- Salinity:
- Not specified (freshwaterused).
- Conductivity:
- Not specified.
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The EC50 at 48hours is estimated close to 100mg/L.
Any other information on results incl. tables
Table 1: Physicochemicalparameters
Control |
||||
Temps |
Hardness (mg/L) |
Oxygene level (%) |
Oxygene level (mg/L) |
pH |
T 0 hour |
289 |
98 |
8.8 |
8.32 |
T 48 hours |
306 |
99 |
8,9 |
8,33 |
Highest concentration tested |
||||
Temps |
Oxygene level (%) |
Oxygene level (mg/L) |
pH |
Nominal concentration (mg/L) |
T 0 hour |
99 |
8.9 |
8.95 |
100 |
T 48 hours |
98 |
8.8 |
8.42 |
100 |
Temperature measured in the dilution water between T0 hour and T2 hours: 19.8°C.
Temperature recorded in a beaker of dilution water placed under the conditions of the study: minimum 19.4°C / maximum 19.8°C.
Table 2: Summary of mortality
Immobilisation (n daphnies on 5) at each nominal concentration (mg/L) |
||||||||||||||||
|
0 |
1 |
10 |
100 |
||||||||||||
Replicat |
Replicat |
Replicat |
Replicat |
|||||||||||||
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
|
T0 hour |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
T24 hours |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
T 48hours |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
2 |
0 |
4 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest concentration without immobilization at T48h was 10 mg/L.
The lowest concentration with 100% immobilization at T48h was >100 mg/L.
The 24h EC50 was >100 mg / L.
The EC50 at 48h was estimated to be close to 100 mg / L. - Executive summary:
The objective of this study is to assess the acute toxicity of TMZ BASE, in Daphnia magna STRAUS - clone 5, in a 48-hour static test, according to the 202 OECD guideline.
The test was carried out by subjecting 20 animals to a maximum concentration level of 100 mg/L and further groups of 20 animals to two other test solutions, i.e. 1 and 10 mg/L, over a 48-hour period. The control also contained 20 animals.
The highest concentration without immobilization at T48h was 10 mg/L.
The lowest concentration with 100% immobilization at T48h was >100 mg/L.
The 24h EC50 was >100 mg / L.
The EC50 at 48h was estimated to be close to 100 mg / L.
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