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EC number: 205-026-8 | CAS number: 131-53-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-01-19 - 2018-03-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- April 2004
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- March 2015
Test material
- Reference substance name:
- Dioxybenzone
- EC Number:
- 205-026-8
- EC Name:
- Dioxybenzone
- Cas Number:
- 131-53-3
- Molecular formula:
- C14H12O4
- IUPAC Name:
- 2-(2-hydroxybenzoyl)-5-methoxyphenol
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Name: 2,2’ -Dihydroxy-4-methoxybenzophenone
Batch/Lot Number: CM70314A1
Description: Yellow powder
Expiry Date: 14 March 2019
Purity: 99.8%
Storage Conditions: Room Temperature, protected from light
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 50.0, 25.0, 12.5 and 6.25 % of a filtrate at a loading rate of 100 mg test item/L
- Sampling method: Control and test solutions were sampled in duplicate at the start of exposure and at 48h.
- Sample storage conditions before analysis: All samples were stored in amber glass bottles in the dark at a temperature of ≤ -18°C after sampling and before shipment.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
As the test item is poorly water-soluble, the following approach was used taking into account aspects of ISO 14442 (2006), Water quality -- Guidelines for algal growth inhibition tests with poorly soluble materials, volatile compounds, metals and waste water, and OECD 2000, Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures. OECD Series on Testing and Assessment Number 23. ENV/JM/MONO(2000)6.
A stock solution was prepared at a nominal loading rate of 100 mg/L as described below by adding 0.10038 mg of the test item to 1000 mL of the test medium. This stock solution was stirred with 600 rpm for 48 hours at ambient temperature in the dark.
Thereafter this stock solution was filtered, using a glass fibre filter with an average retention capacity of 0.40 µm (MN 85-220). The initial 150 mL of the filtrate were discarded. Visual inspection of the flask showed a clear slightly yellow solution without Tyndall effect.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: M10
- Source: Daphnia magna STRAUS (clone M10), a water flea, cultured at ECT Oekotoxikologie GmbH since December 22, 2011
- Age of parental stock: < 35 days
- Feeding during test: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 254 mg/L CaCO3
- Test temperature:
- 20.3 - 21.0 °C
- pH:
- 7.7 - 7.9
- Dissolved oxygen:
- 89 - 93 % of air saturation
7.9 - 8.2 mg/L - Conductivity:
- 676 µS/cm
- Nominal and measured concentrations:
- control and 100, 50.0, 25.0, 12.5 and 6.25 % of a filtrate at a loading rate of 100 mg test item/L (nominal concentrations)
(measured concentrations) - Details on test conditions:
- TEST SYSTEM
- Test vessel:100-mL glass beakers, closed by watch glass
- Material, size, headspace, fill volume: 50-60 mL fill volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Test medium: Elendt medium M4
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16h light / 8h dark
- Light intensity: 580-638 lx - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.1 other: % of filtrate at 100 mg test item/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 4.27 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: Sublethal observations on the mobile daphnids were recorded. At 25% of filtrate at 100 mg test item/L reduced swimming activity was observed after 24 h and in respectively one test vessel at 12.5 and 25.0% of filtrate at 100 mg test item/L after 48 h.
- Mortality of control: 0%
- Other adverse effects in controls: None
Validity criteria:
% of immoible daphnids in the control was less than 10% (0%) and dissolved oxygen concentration in control and test vessels at the end of the test were greater than 3 mg/L (> 7.9 mg/L) - Results with reference substance (positive control):
- 24h EC50 0.74 mg/L
The results of this reference test are acceptable and the test conditions are reliable and the sensitivity of the test system could be demonstrated.
Any other information on results incl. tables
Table 1: Cumulative number of immobile Daphnia magna after 24 and 48 h
nominal concentration |
total number of introduced daphnids |
total number of immobilised daphnids |
immobilised daphnids (% of introduced) |
total number of immobilised daphnids |
immobilised daphnids (% of introduced) |
[%*] |
|
[24 h] |
[24 h] |
[48 h] |
[48 h] |
Control |
20 |
0 |
0.0 |
0 |
0.0 |
6.25 |
20 |
0 |
0.0 |
0 |
0.0 |
12.5 |
20 |
0 |
0.0 |
0 |
0.0 |
25.0 |
20 |
13 |
65.0 |
19 |
95.0 |
50.0 |
20 |
20 |
100.0 |
20 |
100.0 |
100 |
20 |
20 |
100.0 |
20 |
100.0 |
*% of filtrate at 100 mg test item/L
Table 2: Summary of measured concentrations
% of a saturated solution with a loading rate of 100 mg/L |
Sample name |
Test period [d] |
Age of test matrix [d] |
Measured conc (mg/L) |
0 |
0530 |
0 |
0 |
<LOD |
6.25 |
0532 |
0 |
0 |
1.25 |
12.5 |
0534 |
0 |
0 |
2.57 |
25 |
0536 |
0 |
0 |
4.78 |
50 |
0538 |
0 |
0 |
10.3 |
100 |
0540 |
0 |
0 |
20.6 |
0 |
0543 |
2 |
2 |
<LOQ |
6.25 |
0545 |
2 |
2 |
1.18 |
12.5 |
0547 |
2 |
2 |
2.38 |
25 |
0549 |
2 |
2 |
4.76 |
50 |
0551 |
2 |
2 |
10.2 |
100 |
0553 |
2 |
2 |
20.1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 value for daphnids exposed to the test item was ca. 4.27 mg/L.
- Executive summary:
The acute toxicity of the dioxybenzone was tested according to OECD guideline 202 and GLP conditions.
A clear concentration-response relationship was observed. The following ECxvalues were determined based on nominal concentrations in % of filtrate at 100 mg test item/L:
EC10: 18.5 % of filtrate at 100 mg test item/L; ca. 3.73 mg/L
EC20: 19.3 % of filtrate at 100 mg test item/L; ca. 3.90 mg/L
EC50: 21.1 % of filtrate at 100 mg test item/L; ca. 4.27 mg/L
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