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EC number: 232-577-1 | CAS number: 9001-05-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 February 1992 to 3 December 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Catalase belongs to the same sub-subclass as peroxidase and due to the similarity of the two enzymes, read-across can be applied.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Guideline No. 202, Part 1 and EEC Directive 67/548 Annex 5, C2 as published in 84/449/EEC
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Active enzyme protein of peroxidase (EC no. 232-668-6, CAS no. 9001-05-2, EC name Peroxidase, Enzyme class no. 1.11.1.7)
- Molecular formula:
- Not applicable, see remarks.
- IUPAC Name:
- Active enzyme protein of peroxidase (EC no. 232-668-6, CAS no. 9001-05-2, EC name Peroxidase, Enzyme class no. 1.11.1.7)
- Reference substance name:
- Protein as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available
- IUPAC Name:
- Protein as a constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Carbohydrates constituent of enzyme deriving from the fermentation or extraction process.
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Carbohydrates constituent of enzyme deriving from the fermentation or extraction process.
- Reference substance name:
- Lipids as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Lipids as a constituent of enzyme deriving from the fermentation or extraction process
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): SP 491 Peroxidase
- Substance type: UVCB
- Physical state: Dark brown crystaline solid
- Lot/batch No.: PPX 3806
- Expiration date of the lot/batch: 04 March 1994
- Storage condition of test material: In the refrigerator at 4 degrees of Celcius
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Constituent 5
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Controls: Laboratory tap water, dechlorinated by passage through activated carbon, softened by reverse osmosis and aged by recirculation though a gravel filter bed. Total hardness 150 - 200 mg/I as CaCO3.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: Laboratory culture originating from a strain supplied by IRChA, France.
- Age at study initiation: Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.
- Feeding during test: No
- Food type: Cultures were fed daily with a mixture of fry fish food (Liquifry®) and a suspension of mixed algae (predominantly Scenedesmus or Selenastrum spp.).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 150-200 mg/L CaCO3
- Test temperature:
- 21°C
- pH:
- 7.6-8
- Dissolved oxygen:
- 7.7-8.6 mg O2/L
- Conductivity:
- 1000 pS/cm
- Nominal and measured concentrations:
- 5.6, 10, 18, 32, 56, 100, 180, 320 and 560 mg/L (nominal concentrations)
- Details on test conditions:
- TEST SYSTEM
- Test vessels: Glass jars each containing 200 ml test solution.
- Experimental design: 9 test concentrations plus 1 control each in duplicate. 20 animals per test concentration.
- Aeration: None.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water, dechlorinated by passage through activated carbon, softened by reverse osmosis and aged by recirculation though a gravel filter bed. Total hardness 150 - 200 mg/I as CaCO3.
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
Method of initiation: Daphnia were placed in the test solutions after addition of the test substance.
Adjustment of pH: No
Loading: 20 mL test solution per organism.
Photoperiod: 16 h light: 8 h dark.
Medium renewal: None.
Duration of exposure: 48 hours.
Criterion of effect: Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The numbers of mobile, immobile and floating daphnia were counted approximately 24 and 48h after the start of the study.
TEST CONCENTRATIONS
- 5.6, 10, 18, 32, 56, 100, 180, 320 and 560 mg/L (1.6, 2.8, 5.1, 16, 28.6, 91.4, 160 mg/L active enzyme protein). - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 95 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 26.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 560 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 154.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 8.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 560 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 154.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- Details on results
- Mortality of control: 0
- Other adverse effects control: no
- Abnormal responses: no
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Analysis of the immobility of Daphnia magna exposed to peroxidase to concentrations up to 560 mg/L for 48 hours gave an EC50 value of 95 mg/L, equivalent to 26.2 mg/L active enzyme protein.
- Executive summary:
The acute toxicity of Peroxidase to Daphnia magna was assessed under static exposure conditions.
The study was conducted in accordance with OECD Guideline No. 202, Part 1 and EEC Directive 67/548 Annex 5, C2 as published in 84/449/EEC. A group of twenty Daphnia ≤24 hours old was exposed for 48 hours to Peroxidase in concentrations up to 560 mg/L. Dechlorinated tap water was used as test water, having a total hardness of 150 -200 mg/L.
Observations of the Daphnia in each control and test vessel were made after 24 and 48 hours, which produced these findings:
24-hour EC50 value: >560 mg/L (>154.6 mg/L active enzyme protein)
48-hour EC50 value: 95 mg/L (26.2 mg/L active enzyme protein)
24-hour NOEC value: >560 mg/L (>154.6 mg/L active enzyme protein)
48-hour NOEC value: 32 mg/L (8.8 mg/L active enzyme protein)
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