beta-D-fructofuranosyl alpha-D-glucopyranoside octa(acetate and palmitate and stearate)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 Dec 2004 - 05 Jan 2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well-documented study done according to Japanese Industrial Standard (JIS) K 0102: 1998 "Testing methods for industrial wastewater". Fish were only exposed for 48 h, and only nominal concentrations were reported.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Japanese Industrial Standard (JIS) K 0102: 1998 "Testing methods for industrial wastewater"

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sucrose tetrastearate triacetate

IUPAC Name:

Sucrose tetrastearate triacetate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added at a concentration of 10000 mg/L.
- Controls: dilution water

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: medaka
- Source: Japan Food Research Laboratories
- Length at study initiation (length definition, mean, range and SD): 2.2 cm
- Weight at study initiation (mean and range, SD): 0.09 g

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): <5% mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

62 mg/L CaCO3

Test temperature:

24 °C

pH:

8.0

Nominal and measured concentrations:

nominal: control, 10000 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel: 5 L round glass vessel
- Material, size, headspace, fill volume: 4 L
- Aeration: Yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water

OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: None

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The 48-hr LC50 of the test substance was >10000 mg/L.

Executive summary:

The acute toxicity of the test substance to fish was determined in a limit test. 10 fish were exposed to a concentration of 10000 mg/L of test substance. No mortality was observed in either the test or control groups. The fish 48-hr LC50 of the test substance is >10,000 mg/L.