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EC number: 235-527-7 | CAS number: 12262-58-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Adequate details; no COA; non-GLP. Needs test article id statement
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: FDA - Draize Test
- Deviations:
- not specified
- GLP compliance:
- no
Test material
- Reference substance name:
- Cyclohexanone, peroxide
- EC Number:
- 235-527-7
- EC Name:
- Cyclohexanone, peroxide
- Cas Number:
- 12262-58-7
- Molecular formula:
- Multi-constituent substance (equilibrium mixture of a number of cyclohexyl based organic peroxides, hydrogen peroxide, cyclohexanone and water)
- IUPAC Name:
- Peroxycyclohexanone
- Reference substance name:
- Cyclonox LE
- IUPAC Name:
- Cyclonox LE
- Test material form:
- other: colorless liquid
- Details on test material:
- None provided. Only trade name and physical form provided. The purity was not provided. However, it is assumed to be 21% based on the information provided in another acute report, conducted during the same time frame, at the same laboratory.
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- None provided.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- One tenth of a milliliter
- Observation period (in vivo):
- The eyes are examined at 24, 48, 72 hours and 7 days after instillation of the test material.
- Number of animals or in vitro replicates:
- one
- Details on study design:
- The eyes of the animals are examined before testing and only those animals without observable eye defects are used. On tenth of a milliliter of the test substance is allowed to fall on the everted lower lid of one eye of each rabbit; the upper and lower eye lid are then carefully closed and subsequently held together for at least one second before releasing to prevent loss of material. The other eye remaining untreated serves as a control. The eyes are not washed following installation and the animals are released immediately.
The eyes are examined at 24, 48, 72 and 7 days after instillation of the test material.
An animal is considered as giving a positive reaction if there is, at any of the readings, discernible opacity of the cornea (other than a slight dulling of the normal lustre), or ulceration of the cornea, or inflammation of the iris (other than a slight deepening of the folds (rugae) or a slight circumcorneal injection), or if such substances produce in the conjunctivae (palpebral and bulbar, excluding the cornea and iris) an obvious swelling with partial eversion of the lids, or a diffuse deep-crimson red with individual vessels not easily discernible. The FDA scoring scale was used.
Ocular reactions are read using a binocular magnifying glass. The diagnosis of corneal damage is confirmed if necessary by staining the eyes of the animals with fluorescein-impregnated papers. After flushing the excess fluorescein solution, the eyes are examined in a dark room under ultraviolet illumination.
A substance which has elicited corneal and/or iris lesions which have not cleared by the seventh day reading, is considered a severe eye irritant.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: only one animal used
- Time point:
- 24 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- iris score
- Basis:
- animal: only one animal used
- Time point:
- 24 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: only one used
- Time point:
- 24 h
- Max. score:
- 3
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Remarks on result:
- other: conjunctivae were necrotic
- Irritation parameter:
- chemosis score
- Basis:
- animal: only one used
- Time point:
- 24 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: only one used
- Time point:
- 48 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- iris score
- Basis:
- animal: only one used
- Time point:
- 48 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: only one used
- Time point:
- 48 h
- Max. score:
- 3
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Remarks on result:
- other: conjunctivae were necrotic
- Irritation parameter:
- chemosis score
- Basis:
- animal: only one used
- Time point:
- 48 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: only one used
- Time point:
- 72 h
- Score:
- >= 3 - <= 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- iris score
- Basis:
- animal: only one used
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- other: i
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: onnly one used
- Time point:
- 72 h
- Max. score:
- 3
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Remarks on result:
- other: conjunctivae were necrotic
- Irritation parameter:
- chemosis score
- Basis:
- animal: only one used
- Time point:
- 72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: only one used
- Time point:
- 7 d
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Irritation parameter:
- iris score
- Basis:
- animal: only one used
- Time point:
- 7 d
- Remarks on result:
- other: iris not discernable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: only one used
- Time point:
- 7 d
- Max. score:
- 3
- Reversibility:
- not reversible
- Remarks:
- after 7 days
- Remarks on result:
- other: conjunctivae were necrotic
- Irritation parameter:
- chemosis score
- Basis:
- animal: only one used
- Time point:
- 7 d
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- not reversible
- Remarks:
- after 7days
- Irritant / corrosive response data:
- Moderate to complete corneal opacity, slight iritis,
necrosis of the conjunctivae and swelling with lids
about half closed.
The ocular lesions suggested permanent blindness.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- The test article caused very severe irreversible eye damage.
- Executive summary:
The test article was evaluated for potential eye irritation. One tenth of a milliliter was instilled into one eye of one rabbit. Reactions were evaluated at 24,48, 72 hours and 7 days following instillation.
The test article induced very severe irreversible eye damage.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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