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EC number: 306-621-6 | CAS number: 97338-28-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Toxicity control from biodegradation study.
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Aug - 07 Sep 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The solution of the reference substance in mineral medium (100 mg/L) was prepared using a stock solution (10 g/L). Required amounts of the test item were added directly to the test vessels and the mineral medium was added subsequently. - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source of inoculum/activated sludge: Sewage treatment plant Ruhrverband Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany
- Date of sampling: 10th August 2016
- Storage conditions: No storage
- Preparation of inoculum for exposure: No washing, the concentration was adjusted to 3.0 g/L and verified by dry mass measurement.
- Pretreatment: Kept aerobic until use
- Concentration of sludge in the test: 29.6 mg dry mass/litre - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 28 d
- Test temperature:
- 22 ± 1 °C
- pH:
- 7.4 ± 0.2 (test start)
7.8 (test end) - Nominal and measured concentrations:
- 20, and 100 mg/L test item (nominal) corresponding to 61, and 305 mg ThOD/L
- Details on test conditions:
- TEST SYSTEM
- Toxicity control: Containing 100 mg/L test item and 100 mg/L reference item (2 replicates)
- Test vessel: Respirometer flasks (SAPROMAT respirometer (VOITH Inc.)
- Material, size, headspace, fill volume: 500 mL respirometer flasks filled with 250 mL test solution.
- Aeration: The suspension was aerated during the whole test.
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates): 2
- Sludge concentration: 29.6 mg dry mass/litre
- Other: The solutions were stirred throughout the test.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water, free from inhibitory concentrations of toxic substances (e.g. Cu2+ ions)
OTHER TEST CONDITIONS
- Adjustment of pH: Before test start the pH of all solutions were determined and adjusted to 7.4 ± 0.2 with H2SO4 (50 g/L).
- Photoperiod: 24 h darkness
EFFECT PARAMETERS MEASURED:
Oxygen consumption was measured and recorded continuously throughout the duration of the test using a SAPROMAT respirometer (VOITH Inc.). - Reference substance (positive control):
- yes
- Remarks:
- sodium benzoate
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other:
- Remarks:
- Toxicity control from a biodegradation study.
- Details on results:
- In absence of a microbial inhibition test, a NOEC for microbial toxicity can be derived from a ready biodegradability test. According to Chapter R.7b: Endpoint specific guidance (ECHA, 2017) if a compound degrades well in a ready biodegradability test, or does not inhibit the degradation of a positive control at a certain concentration, this concentration can be used as a NOEC value.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 100 mg/L (nominal).
Reference
The test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration.
Percent degradation. Degradation (%) after 14 days.
vessel |
|
Test suspension 100 mg/L |
Test suspension 20 mg/L |
Abiotic |
Procedural control |
Toxicity |
1 |
|
45.2 |
88.4 |
0.0 |
86.5 |
42.8 |
2 |
|
45.9 |
78.6 |
0.0 |
84.7 |
47.5 |
Mean |
|
45.5 |
83.5 |
0.0 |
85.6 |
45.1 |
SD |
|
0.5 |
6.9 |
0.0 |
1.3 |
3.3 |
Percent degradation. Degradation (%) after 28 days.
vessel |
|
Test suspension 100 mg/L |
Test suspension 20 mg/L |
Abiotic |
Procedural control |
Toxicity |
1 |
|
67.5 |
88.4 |
0.0 |
91.3 |
57.4 |
2 |
|
65.5 |
83.5 |
0.0 |
91.3 |
64.2 |
Mean |
|
66.5 |
86.0 |
0.0 |
91.3 |
60.8 |
SD |
|
1.4 |
3.5 |
0.0 |
0.0 |
4.8 |
Description of key information
NOEC (14 d) ≥ 100.0 mg/L for activated sludge microorganisms (OECD 301 F)
Key value for chemical safety assessment
Additional information
There is no respiration inhibition study available assessing the toxicity of Isohexadecyl 12-[(1-oxooctadecyl)oxy]octadecanoate (CAS 97338-28-8) to activated sludge microorganisms. However, a standard ready biodegradability test conducted according to OECD guideline 301 F and GLP is available, whose toxicity control can be used to derive a NOEC. According to the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA, 2017), a concentration can be used as NOEC value, if a compound degrades well in a ready biodegradability test, or does not inhibit the degradation of a positive control at a certain concentration.
The available ready biodegradability test included a toxicity control containing 100 mg/L test item and 100 mg/L reference item (benzoic acid, sodium salt) to determine the possible toxicity to microorganisms of activated sludge at a concentration of 29.6 mg dry mass/L.
After 14 d, a mean biodegradation of 45.1% was observed and after 28 d a mean biodegradation of 60.8% was recorded in the toxicity control. In the procedural controls, the reference substance sodium benzoate was degraded by an average of 86% by exposure day 14, and reached an average biodegradation of 91% by the end of the test (day 28), thus confirming the suitability of the activated sludge. Hence, Isohexadecyl 12-[(1-oxooctadecyl)oxy]octadecanoate (CAS 97338-28-8) had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration and a NOEC (14 d) of ≥ 100.0 mg/L (nominal) was derived.
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