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EC number: 241-997-4 | CAS number: 18096-62-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 - 19 Jan 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Each animal is additionally treated with 3 different dilutions of test substance. No analytical purity of test substance given.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- (1981)
- Deviations:
- yes
- Remarks:
- each animal is additionally treated with 3 different dilutions of test substance
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- Version / remarks:
- (Commission Directive 84/449/EEC)
- Deviations:
- yes
- Remarks:
- each animal is additionally treated with 3 different dilutions of test substance
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4,4a,5,9b-tetrahydroindeno[1,2-d]-1,3-dioxin
- EC Number:
- 241-997-4
- EC Name:
- 4,4a,5,9b-tetrahydroindeno[1,2-d]-1,3-dioxin
- Cas Number:
- 18096-62-3
- Molecular formula:
- C11H12O2
- IUPAC Name:
- 2H,4H,4aH,5H,9bH-indeno[1,2-d][1,3]dioxine
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: approx. 12 - 16 weeks
- Weight at study initiation: 2.15 - 2.17 kg
- Housing: individually in metal cages
- Diet: Rabbit Diet (Preston Farmers Limited, Lincolnshire, U.K.), ad libitum
- Water: mains drinking water, ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 17 - 20
- Humidity (%): 59 - 65
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- other: diethyl phthalate/ethanol (1:1)
- Controls:
- not specified
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 0.5 g
- Concentrations: 2, 10, 50% (w/w) and 100% - Duration of treatment / exposure:
- 4 hours
- Observation period:
- 7 days
Reading time point: 1, 24, 48, 72 hours and 7 days - Number of animals:
- 2 males and 1 female
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm
- Type of wrap if used: The treated skin was covered with a gauze patch and held in place with surgical adhesive tape.
REMOVAL OF TEST SUBSTANCE
- Washing: Skin sites were gently swabbed with cotton wool soaked in ethanol.
- Time after start of exposure: 4 hours
SCORING SYSTEM: Draize scoring system
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal: #1, #2 and #3
- Time point:
- 24/48/72 h
- Score:
- 0.67
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Remarks on result:
- other: undiluted test substance
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 hours
- Remarks on result:
- other: undiluted test substance
- Irritation parameter:
- edema score
- Basis:
- animal: #2 and #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: reversiblitiy: not applicable
- Remarks on result:
- other: undiluted test substance
- Irritant / corrosive response data:
- Undiluted test substance: No adverse skin reactions were noted at any treated skin site 1 hour after patch removal. Very slight erythema was noted at the time points 24 and 48 hours. Desquamation was noted at two treatment sites at the 72-hour observation and at one treatment site at the 7-day observation. Very slight oedema was noted at one treatment site at the 24-hour observation.
50% dilution: Very slight erythema was noted at the reading time points 24 and 48 hours and at two treatment sites at the 72-hours observation. Desquamation was noted at two treatment sites at the 7-day observation. Very slight oedema was noted at all treatment sites at the 24-hour observation and at one treatment site at the 48-hour observation.
10% dilution: Very slight erythema was noted at one treatment site one hour after patch removal and at all treatment sites at the 24-and 48-hour observations. Very slight erythema or desquamation was noted at two treatment sites at the 72-hour observation. Desquamation only was noted at these sites at the 7-day observation. Slight oedema was noted at one treatment site at the 24-hour observation with very slight oedema at the 48-hour observation.
2% dilution: Very slight erythema was noted at one treatment site one hour after patch removal. Very slight or well-defined erythema was noted at all treatment sites at the 24-hour observation. Very slight erythema was noted at two treatment sites at the 48 and 72-hour observations with desquamation only at these sites at the 7-day observation. Slight oedema was noted at one treatment site at the 24-hour observation with very slight oedema at the 48-hour observation.
Any other information on results incl. tables
Table 1: Individual skin examination scores with 100, 50, 10 and 2% test substance
Animal |
Concentration % |
Erythema/Eschar formation |
Oedema formation |
||||||||||
1 h |
24 h |
48 h |
72 h |
7 d |
Mean 24/48/72 h |
1 h |
24 h |
48 h |
72 h |
7 d |
Mean 24/48/72 h |
||
1 (male) |
100% (undiluted) |
0 |
1 |
1 |
0D |
0D |
0.67 |
0 |
1 |
0 |
0 |
0 |
0.33 |
50% |
0 |
1 |
1 |
0 |
0 |
0.67 |
0 |
1 |
0 |
0 |
0 |
0.33 |
|
10% |
0 |
1 |
1 |
0D |
0D |
0.67 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2% |
0 |
1 |
0 |
0 |
0 |
0.33 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2 (male) |
100% (undiluted) |
0 |
1 |
1 |
0 |
0 |
0.67 |
0 |
0 |
0 |
0 |
0 |
0 |
50% |
1 |
1 |
1 |
1 |
0D |
1 |
0 |
1 |
1 |
0 |
0 |
0.67 |
|
10% |
0 |
1 |
1 |
0 |
0 |
0.67 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2% |
1 |
1 |
1 |
1 |
0D |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
|
3 (female) |
100% (undiluted) |
0 |
1 |
1 |
0D |
0 |
0.67 |
0 |
0 |
0 |
0 |
0 |
0 |
50% |
0 |
1 |
1 |
1 |
0D |
1 |
0 |
1 |
0 |
0 |
0 |
0.33 |
|
10% |
1 |
1 |
1 |
1 |
0D |
1 |
0 |
2 |
1 |
0 |
0 |
1 |
|
2% |
0 |
2 |
1 |
1 |
0D |
1.33 |
0 |
2 |
1 |
0 |
0 |
1 |
D: desquamation
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No 1272/2008
- Conclusions:
- Under the conditions of this skin irritation study in rabbits the undiluted test substance (100%) was not irritating to the skin. Also the test substance concentrations of 2, 10 and 50% in ethanol/diethylphthalat (1:1) did not show an irritation potential.
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