Benzenesulfonamide, 4-methyl-, reaction products with formaldehyde and melamine

Endpoint summary

Description of key information

Short term toxicity to invertebrates:

1. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be >100 mg/L.

2. Based on the immobility of daphnia magna by the exposure with the test chemical for 24 hrs, the EC0 was determine to be > 2500 mg/l.

3. Based on the immobility of daphnia magna by the test chemical, the EC50 value in 24 hrs was determined to be 109.13 mg/l.

Thus based on above data, the chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Toxicity to aquatic algae:

1. After 72 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be >200 mg/l graphically and through probit analysis.

2. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 110.6 mg/L on the basis of effects on growth rate in a 72 hour study.

3. The median effective concentration (ErC50) for the test substance, in Desmodesmus subspicatus was determined to be 110 mg/L on the basis of effects on growth rate in a 72 hour study.

Thus based on the overall studies for Benzenesulfonamide, 4-methyl-, reaction products with formaldehyde and melamine (97808 -67 -8), it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria.  

Additional information

Summarized result for the determination of nature of chemical Benzenesulfonamide, 4-methyl-, reaction products with formaldehyde and melamine (97808 -67 -8) on the growth and other biological and physical activity of aquatic invertebrates, algae and cyanobacteria when chemical comes in contact with test organisms, by considering the data for structurally and functionally similar read across chemicals from various sources which are as follows:  

 

Short term toxicity to invertebrates:

Short term toxicity of Benzenesulfonamide, 4-methyl-, reaction products with formaldehyde and melamine (97808 -67 -8) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Daphnia sp., Acute Immobilization Test according to OECD Guideline 202 was conducted for test chemical to assess the toxic effects of the test compound on the test Daphnids. Beaker containing 20ml of media with 10 Daphinds. A semi-static procedure was used for the study. The nominal concentration selected for the experiment was 100 mg/L and test Daphnids were exposed to this concentration for 48hours. The Effective concentrations EC50 was found to be >100 mg/L. Thus, according to the CLP Criteria for aquatic classification of thesubstance, it is concluded that test chemicaal does not exhibit toxicity to aquatic invertebrate (daphnia magna) andtherefore it cannot be classified as hazardous substance.

 

Similar study was performed for another structurally and functionally similar read across chemicals. Aim of this study was to investigate the effect of test chemical on the growth of aquatic invertebrates. Test conducted according to the standard OECD guidelines with the total exposure period of 24 hrs. Freshwater daphnia magna were used as a test organism. After the exposure period no effect were observed. Based on the immobility of daphnia magna by the exposure with the test chemical for 24 hrs, the EC0 was determine to be > 2500 mg/l. Thus based on the EC0, chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

Similarly Aim of this study was to determine the short term toxicity of test chemical on the growth and behavior of freshwater aquatic invertebrate daphnia magna for 24 hrs. Based on the immobility of daphnia magna by the test chemical, the EC50 value in 24 hrs was determined to be 109.13 mg/l. Based on the result, it was concluded that the test chemical was nontoxic and not classified according to the CLP regulations. 

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for Benzenesulfonamide, 4-methyl-, reaction products with formaldehyde and melamine (97808 -67 -8), it can be concluded that the test chemical was nontoxic as value ranges from > 100 to > 2500 mg/l and not classified as per the CLP classification criteria.  

 

 

Toxicity to aquatic algae:

Short term toxicity of Benzenesulfonamide, 4-methyl-, reaction products with formaldehyde and melamine (97808 -67 -8) on the growth and other biological activity of aquatic algae is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the algae. The effect of test item was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200 mg/L. Thus, based on this EC50 value and according to CLP Criteria for aquatic classification of the substance, it is concluded that test chemical does not exhibits toxicity to aquatic algae (Chlorella vulgaris).

 

Similar study was performed for another structurally and functionally similar read across chemicals. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance according to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 0, 30, 45, 67, 100, 150 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 110.6 mg/L. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP criteria.

 

Study was performed for another structurally and functionally similar read across chemicals. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance according to OECD Guideline 201. The stock solution 150 mg/l was prepared by dissolving light brown powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Sample tested at the concentrations 0, 0, 30, 45, 67, 100 and 150 mg/L. Test performed by the static system for 72hrs of exposure period. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, in Desmodesmus subspicatus was determined to be 110 mg/L on the basis of effects on growth rate in a 72 hour study. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as toxic as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for Benzenesulfonamide, 4-methyl-, reaction products with formaldehyde and melamine (97808 -67 -8), it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria.