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EC number: 235-920-3 | CAS number: 13047-13-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 August 2017 to 01 September 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO International Standard 10634. "Water Quality - Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium", (1995)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands
- Storage conditions: kept under aeration
- Settlement length: approximately 47 minutes
- Pretreatment: allowed to settle, the supernatant is used as inoculum
- Concentration of sludge: 10 mL/L medium
- suspended solids: 3.2 g/L - Duration of test (contact time):
- 29 d
- Initial conc.:
- 18.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to the guideline
- Test temperature: 22.3 - 23.2°C.
- pH: 7.5-7.6 (positive control 7.6-7.8)
- pH adjusted: yes with 1M HCl (initial pH 7.8)
- Aeration of dilution water: yes
- Suspended solids concentration: 3.2 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 litre brown glass vessels
- Number of culture flasks/concentration: 2 for inoculum controls, 2 for test substance, 1 for reference substance, 1 for toxicity control
- Method used to create aerobic conditions: Synthetic air was sparged through the solutions
- Measuring equipment: A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The synthetic air was sparged through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
- Test performed in closed vessels due to significant volatility of test substance: NA
- Details of trap for CO2: 3 BaOH traps in serie
SAMPLING: day 1, 4, 6, 8, 11, 15, 18, 22, 25 and 29 (+after elution with HCl)
STATISTICAL METHODS: none applied - Reference substance:
- acetic acid, sodium salt
- Remarks:
- 40 mg/L, 12 mg/L as TOC
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 29 d
- Details on results:
- 1. The positive control item was biodegraded by at least 60% (70%) within 14 days.
2. The difference of duplicate values for %-degradation of the test item was always less than 20 (≤ 0%).
3. The total CO2 release in the blank at the end of the test did not exceed 40 mg/L (59.1 mg CO2 per 2 litres of medium, corresponding to 29.6 mg CO2/L).
4. The Inorganic Carbon content (IC) of the test item (suspension) in the mineral medium at the beginning of the test was less than 5% of the Total Carbon content (TC). Since the test medium was prepared in tap-water purified by reverse osmosis (Milli-RO water (Millipore Corp., Bedford, Mass., USA, carbon levels < 500 ppb)), IC was less than 5% of TC (mainly coming from the test item, 12 mg TOC/L). - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is considered not readily biodegradable under the conditions of the test
- Executive summary:
CH01610 was tested in duplicate at a target concentration of 18.5 mg/L, corresponding to 12 mg TOC/L. The organic carbon content was based on the molecular formula. The study consisted of six bottles:
· 2 inoculum blanks (no test item),
· 2 test bottles (CH01610),
· 1 positive control (sodium acetate) and
· 1 toxicity control (CH01610 plus sodium acetate).
Weighed amounts of the substance were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Furthermore, the test medium was daily swirled around, since the test item tended to float on the water surface. Test duration was 28 days for the inoculum blank and test item (last CO2measurement on day 29) and 14 days for the positive and toxicity control (last CO2measurement on day 15).
The relative biodegradation values calculated from the measurements performed during the test period revealed no biological relevant biodegradation of CH01610 (0% in both test vessels).
In the toxicity control, CH01610 was found not to inhibit microbial activity. All criteria for acceptability of the test were met.
In conclusion, CH01610 was designated as not readily biodegradable.
Reference
CO2production and percentage biodegradation
Day |
HCl (0.05 N) titrated (mL) |
Cumulative CO2 (mg) Bottle A |
Cumulative CO2 (mg) Bottle B |
Biodegradation1) (%) Bottle A |
Biodegradation1) (%) Bottle B |
||
Blank (mean) |
Bottle A |
Bottle B |
|||||
1 |
46.91 |
47.53 |
47.48 |
0.0 |
0.0 |
0 |
0 |
4 |
43.65 |
45.13 |
44.96 |
0.0 |
0.0 |
0 |
0 |
6 |
44.28 |
45.75 |
45.76 |
0.0 |
0.0 |
0 |
0 |
8 |
44.87 |
46.48 |
46.26 |
0.0 |
0.0 |
0 |
0 |
11 |
46.59 |
47.43 |
47.13 |
0.0 |
0.0 |
0 |
0 |
15 |
45.16 |
46.78 |
46.31 |
0.0 |
0.0 |
0 |
0 |
18 |
45.31 |
46.11 |
46.35 |
0.0 |
0.0 |
0 |
0 |
22 |
43.88 |
44.80 |
45.60 |
0.0 |
0.0 |
0 |
0 |
25 |
44.82 |
44.95 |
45.75 |
0.0 |
0.0 |
0 |
0 |
292) |
43.55 |
44.68 |
43.41 |
0.0 |
0.1 |
0 |
0 |
292) |
46.26 |
46.74 |
47.45 |
0.0 |
0.1 |
0 |
0 |
292) |
48.77 |
48.53 |
48.76 |
0.3 |
0.2 |
0 |
0 |
1): Calculated as the ratio between CO2 produced (cumulative) and the ThCO2 of the test item: 87.3 mg CO2/2L.
2): CO2 measured on day 29 is actually part of CO2 production of day 28, since microbial activity was ended on day 28 by addition of HCl.
Description of key information
The substance was tested in duplicate at a target concentration of 18.5 mg/L, corresponding to 12 mg TOC/L. The organic carbon content was based on the molecular formula.The study consisted of six bottles:
· 2 inoculum blanks (no test item),
· 2 test bottles (CH01610),
· 1 positive control (sodium acetate) and
· 1 toxicity control (CH01610 plus sodium acetate).
Weighed amounts of the substance were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Furthermore, the test medium was daily swirled around, since the test item tended to float on the water surface. Test duration was28 days for the inoculum blank and test item (last CO2measurement on day 29) and 14 days for the positive and toxicity control (last CO2measurement on day 15).
The relative biodegradation values calculated from the measurements performed during the test period revealed no biological relevant biodegradation of the substance (0% in both test vessels).
CH01610was tested in duplicate at a target concentration of 18.5 mg/L, corresponding to 12 mg TOC/L. The organic carbon content was based on the molecular formula.The study consisted of six bottles:
· 2 inoculum blanks (no test item),
· 2 test bottles (CH01610),
· 1 positive control (sodium acetate) and
· 1 toxicity control (CH01610 plus sodium acetate).
Weighed amounts of the substance were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Furthermore, the test medium was daily swirled around, since the test item tended to float on the water surface. Test duration was28 days for the inoculum blank and test item (last CO2measurement on day 29) and 14 days for the positive and toxicity control (last CO2measurement on day 15).
The relative biodegradation values calculated from the measurements performed during the test period revealed no biological relevant biodegradation of CH01610 (0% in both test vessels).
In the toxicity control, CH01610 was found not to inhibit microbial activity.All criteria for acceptability of the test were met.
In conclusion,CH01610 was designated as not readily biodegradable.
The substance was designated as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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