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EC number: 204-535-2 | CAS number: 122-34-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 February - 17 March 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- traditional method
- Limit test:
- yes
Test material
- Reference substance name:
- Simazine
- EC Number:
- 204-535-2
- EC Name:
- Simazine
- Cas Number:
- 122-34-9
- Molecular formula:
- C7H12ClN5
- IUPAC Name:
- 6-chloro-N2,N4-diethyl-1,3,5-triazine-2,4-diamine
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- TEST MATERIAL
- Test material: Simazine Technical
- Description: White crystalline powder
- Purity: 97.89% a.i. (provided by Sponsor)
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability: Not provided to testing facility
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Texas Animal Specialties, Humble, TX
- Females nulliparous and non-pregnant: yes
- Age at study initiation: approx. 2 months
- Weight at study initiation: Males: 268 - 327 g; Females: 194 - 229 g
- Housing: suspended, wire bottom, stainless steel cages; one animal per cage
- Diet: PMI™ Lab Diet Formula #5008, available ad libitum except during the exposure period
- Water: municipal water supply available ad libitum except during the exposure period
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2°C (actual 20 - 22°C)
- Humidity (%): 30 - 70% (actual 29 - 86%)
- Air changes (per hr): 10-12
- Photoperiod (hrs dark / hrs light): 12 hour light/ dark cycle
IN-LIFE DATES: From: 3 March 2009 To: 17 March 2009
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Mass median aerodynamic diameter (MMAD):
- 2.8 µm
- Geometric standard deviation (GSD):
- 5.9
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: nose-only stainless steel, dynamic flow inhalation chamber
- Exposure chamber volume: 500 L
- Method of holding animals in test chamber: Polycarbonate tubes
- Source and rate of air: 34.7 air changes per hour
- System of generating particulates/aerosols: The aerosol was generated by a Venturi Aspirator which aspirated the test substance from a coupled motorised revolving disc delivery system, then sprayed the resulting aerosol directly into the exposure chamber.
- Method of particle size determination: Determined twice during the exposure using a cascade impactor at a rate of 7.8 L/minute for 25 seconds.
TEST ATMOSPHERE
- Brief description of analytical method used: The concentration of test substance in the exposure atmosphere was determined gravimetrically twice per hour and nominally at the end of the exposure. The gravimetric concentration was determined by passing a known volume of exposure air through a pre-weighed filter and dividing the amount of test substance deposited on the filter by the volume of air which passed through the filter. The nominal concentration was determined by dividing the loss in weight of the test substance after the exposure by the total volume of air that passed through the chamber.
- Samples taken from breathing zone: yes
TEST ATMOSPHERE
- Particle size distribution:
- 1.25 h distribution: ≤ 0.2 µm - 5% of particles collected
≤ 0.5 µm - 16%
≤ 2.8 µm - 50%
≤ 16.8 µm - 84%
≤ 52.6 µm - 95%
- 2.25 h distribution: ≤ 0.2 µm - 5% of particles collected
≤ 0.6 µm - 16%
≤ 2.9 µm - 50%
≤ 13.9 µm - 84%
≤ 38.6 µm - 95%
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): 1.25 h distribution: MMAD 2.8 µm (GSD 5.9); 2.25 h distribution: MMAD 2.9 µm (GSD 4.9) - Duration of exposure:
- 4 h
- Concentrations:
- 2.21 mg/L
- No. of animals per sex per dose:
- 5 animals per sex
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations for mortality and signs of pharmacological and/or toxicological effects were made frequently on the day of exposure and at least once daily thereafter. Individual bodyweights were recorded just prior to exposure and on Days 7 and 14.
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 2.21 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- There was no mortality during the study.
- Clinical signs:
- other: Prominent in-life observations included activity decrease and piloerection. Animals were asymptomatic by Day 4.
- Body weight:
- Body weight gain was unaffected by the administration of the test substance, except in one female that lost 2 g between Days 7 and 14.
- Gross pathology:
- No abnormalities were observed.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute inhalation LC50 for Simazine Technical was >2.21 mg/L in male and female rats.
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