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EC number: 282-162-4 | CAS number: 84100-84-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Letale Wirkung beim Zebra baerbling Brachydanio rerio (LC 0, LC 50, LC 100; 48 - 96 h), UBA test protocol Mai 1984
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Name: Zebra fish (Danio rerio)
Source: West Aquarium GmbH (Germany)
Date of birth: not reported
Date supplied: not reported
Temperature: 20 - 24 °C
Dissolved oxygen: > 5 mg/L
Feeding: Commercial fish food, daily. Feeding discontinued 24 h prior to test start.
Mortalities during acclimatisation period: < 5 %
Medication: none
Holding and dilution water
Reconstituted water prepared according to the recommendations of ISO (hardness: 13° dH, acidity: 0.1 +/- 0.02 mmol/l, Ca / Mg: 4:1, pH: 8.0 – 8.4, oxygen: >= 90 % of saturation). This freshly prepared standard dilution water was used for the maintenance of the test animals under flow-through conditions and for the preparation of stock and test solutions of the test item. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 13 °dH
- Test temperature:
- 19.5 - 21.5 °C
- pH:
- 4.2 - 7.1
- Dissolved oxygen:
- 8.0 - 9.6
- Nominal and measured concentrations:
- nominal concentrations: 12.5, 17.7, 25.0, 35.4, 50.0, 70.7, 100.0, 2000 mg/L
- Details on test conditions:
- Test vessels: glass aquaria holding 5 L of test media covered by glass plates
Experimental design: 8 test concentrations, 10 fish per test concentration, no feeding during the exposure period
Method of initiation: fish were placed in prepared media
Photoperiod: 16 h light: 8 h dark
Temperature: 19.5 to 21.5 °C
Aeration: gentle aeration via narrow glass tubes
Method of administration: direct weighing
Medium renewal: none
Criteria of effects: absence of response to physical stimulation; in addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub lethal effects compared with control fish were observed - Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 70.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: due to decreased pH
- Details on results:
- The mortality was 100 % at concentrations > 100 mg/L after 24 h. According to the test guidelines acute fish tests should be carried out without adjustment of pH and OECD guideline describes water with a pH 6.0 to 8.5 is preferable for the test. However, the pH decreased in the test from 7.2 to 4.2 during 24 h due to intrinsic property of the test material. Thus it is most likely that the low pH-value is the reason for the mortality and not the toxicity of the substance. Therefore no LC50 could be determined and just a LC0 of >=70.7 mg/L is derived.
- Sublethal observations / clinical signs:
concentration of test item, number of fish: normal swimming behaviour/abnormal swimming behaviour/dead and pH-values
24 h (pH) 48 h (pH) 72 h (pH) 96 h (pH) 12.5 mg/L 10/0/0 (5.5) 10/0/0 (6.0) 10/0/0 (6.1) 10/0/0 (6.5) 17.7 mg/L 10/0/0 (6.3) 10/0/0 (6.4) 10/0/0 (6.5) 10/0/0 (6.5) 25 mg/L 10/0/0 (6.5) 10/0/0 (6.4) 10/0/0 (6.5) 10/0/0 (6.5) 35.4 mg/L 10/0/0 (6.3) 10/0/0 (6.5) 10/0/0 (6.4) 10/0/0 (6.5) 50 mg/L 10/0/0 (6.2) 10/0/0 (5.9) 10/0/0 (5.9) 10/0/0 (5.9) 70.7 mg/L 10/0/0 (6.6) 10/0/0 (5.9) 10/0/0 (5.8) 10/0/0 (5.8) 100 mg/L 10/0/10 (4.6) - - - 2000 mg/L 10/0/10 (4.2) - - - - Conclusions:
- For 2,3,3-trimethyl-3H-indole-5-carboxylic acid the mortality of fish at concentrations > 100 mg/L is probably due to decreased ph-values and not toxicity. Thus no LC50 could be determined and just a LC0 of >=70.7 mg/L is derived.
- Executive summary:
The study was conducted according to UBA test protocol: Letale Wirkung beim Zebrabaerbling Brachydanio rerio, (LC 0, LC 50, LC 100; 48 – 96H) Mai 1984, which is comparable to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' and assesses the acute toxic effects (mortality) of various concentrations of 2,3,3 -trimethyl-3H-indole-5 -carboxylic acid to a freshwater fish species.
The test was conducted for 96 h as a static test and 10 fish (Brachydanio rerio) were tested per concentration. The fishs were exposed to 8 concentrations (12.5, 17.7, 25.0, 35.4, 50.0, 70.7, 100.0, 2000 mg/L (nominal)) of 2,3,3-trimethyl-3H-indole-5-carboxylic acid. During the test a temperature range of 20 - 22 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in the test. The temperature, the pH and the oxygen values were measured at the beginning of the test and every 24 hours thereafter. Mortalities and abnormal behaviour were recorded at 24-hour intervals.
The mortality was 100 % at concentrations > 100 mg/L after 24 h. However, this effect could also be ascribed to the decrease of the pH-value after 24 h (4.6 at 100 mg/L and 4.2 at 2000 mg/L) due to intrinsic properties of 2,3,3-trimethyl-3H-indole-5-carboxylic acid as Brachydanio rerio should be kept at pH-values between 6.0 and 8.0 according to the test guideline. At a concentration of 70.7 mg/L the pH-value is 6.6 after 24 h and 5.8 after 96 h. No mortality is observed at this concentration. Thus it is most likely that the low pH-value is the reason for mortality at 100 mg/L and not the toxicity of the substance. Therefore no LC50 could be determined and just a LC0 of >=70.7 mg/L is derived.
Reference
Description of key information
A study was conducted according to UBA test protocol "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, (LC 0, LC 50, LC 100; 48 – 96H) Mai 1984", which is comparable to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. The test was conducted for 96 h as a static test and 10 fish (Brachydanio rerio) were tested per concentration. The fishs were exposed to 8 concentrations (12.5, 17.7, 25.0, 35.4, 50.0, 70.7, 100.0, 2000 mg/L (nominal)) of 2,3,3-trimethyl-3H-indole-5-carboxylic acid.
The mortality was 100 % at concentrations > 100 mg/L after 24 h. However, this effect could also be ascribed to the decrease of the pH-value after 24 h (4.6 at 100 mg/L and 4.2 at 2000 mg/L) due to intrinsic properties of 2,3,3-trimethyl-3H-indole-5-carboxylic acid as Brachydanio rerio should be kept at pH-values between 6.0 and 8.0 according to the test guideline.
At a concentration of 70.7 mg/L the pH-value is 6.6 after 24 h and 5.8 after 96 h. No mortality is observed at this concentration. Thus it is most likely that the low pH-value is the reason for mortality at 100 mg/L and not the toxicity of the substance. Therefore no LC50 could be determined and just a LC0 of >=70.7 mg/L is derived.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 70.7 mg/L
Additional information
"Should read: LC0 >= 70.7 mg/L"
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