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Diss Factsheets
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EC number: 211-734-8 | CAS number: 692-86-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Bewertung wassergefährdender Stoffe (III), Bestimmung der akuten Bakterientoxizität, Ad-hoc-Arbeitsgruppe 1, LTwS, Nr. 10, Sept 1979
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isopropyl myristate
- EC Number:
- 203-751-4
- EC Name:
- Isopropyl myristate
- Cas Number:
- 110-27-0
- Molecular formula:
- C17H34O2
- IUPAC Name:
- isopropyl myristate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 10 mg TS was dissolved in ethanol absolute and after complete evaporation of the organic solvent, a solution of 10 mg/L in sterile Mill-Q water was prepared; 20 mg/L solution prepared similarly; both diluted (1.25 times) in Milli-Q water and mixed for 15 min with magnetic stirrer; pH was adjusted with NaOH
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Stock culture obtained from RIVM, Bilthoven, The Netherlands
- Method of cultivation: New stock cultures started in 1-week intervals; incubated at 25°C
- Preparation of inoculum for exposure: Extinction of the cultures were measured and adjusted to the final test with sterile saline corresponded with the extinction of a Formazin standard suspension at the value 10
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 18 h
- Remarks on exposure duration:
- ± 2 h
- Post exposure observation period:
- not relevant
Test conditions
- Test temperature:
- 25 °C
- pH:
- adjusted to 7.0
- Nominal and measured concentrations:
- nominal: 0, 0.004, 0.008, 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks
- Type (delete if not applicable): closed with aluminium caps
- Material, size, headspace, fill volume: 100 mL test solution
- No. of organisms per vessel: adjusted to an extinction of 10 at 436 nm
- No. of vessels per concentration (replicates): 3 inoculated, 1 without inoculum
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-Q water
OTHER TEST CONDITIONS
- Adjustment of pH: 7.0 with NaOH
EFFECT PARAMETERS MEASURED: Growth inhibition, measured as extinction at 436 nm - Reference substance (positive control):
- yes
- Remarks:
- methanol
Results and discussion
Effect concentrations
- Duration:
- 18 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: the toxicity threshold of methanol was determined as 7537 mg/L, while the literature TT value of methanol was cited as 6600 mg/L - Reported statistics and error estimates:
- Since all the measured extinction values were very close to the reference, no real toxicity threshold could be determined.
Any other information on results incl. tables
Concentration in mg/L |
Mean extinction at 436 nm |
0.004 |
0.416 |
0.008 |
0.418 |
0.016 |
0.407 |
0.031 |
0.411 |
0.063 |
0.400 |
0.125 |
0.397 |
0.250 |
0.395 |
0.500 |
0.403 |
1.0 |
0.413 |
2.0 |
0.403 |
4.0 |
0.414 |
8.0 |
0.431 |
16.0 |
0.449 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.