Propiononitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21-24/4/1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

no analytical meaurement of tested material concentrations were perfomed

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

no analitycal analysis to verify concentration of chemical. only two replicates of 10 daphnids

Principles of method if other than guideline:

- Principle of test: The acute toxicity of Propionitrile to Daphnia magna was assessed using the methods outlined by the Commitee on Methods for Toxicity Tests with Aquatic Organisms.
- Short description of test conditions: Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination of the test and were within acceptable limits. Static Daphnia bioassay was conducted in 250 ml glass beakers containing 200 ml of well water. These vessels were kept at 20°C (±1.0). The photo­period was controlled to give 16 hours daylight and 8 hours darkness.
- Parameters analysed / observed: 48 h immobilization

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Propiononitrile

EC Number:

203-464-4

EC Name:

Propiononitrile

Cas Number:

107-12-0

Molecular formula:

C3H5N

IUPAC Name:

propanenitrile

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch: Montesanto lot. #34
- Purity test date: not stated
- Purity: 96.1 %

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 4 C
- Solubility and stability of the test substance in water: 9.94% at 20 C

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: dilution in deionized water
- Dilution volume: 100 ml deionized water
- Concentration: 20 mg/ml

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test concentrations were prepared based on total compound received. Deionized water was used in the preparation of all working stock solutions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age of parental stock : <24 hours old)
- Feeding during test: not fed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

255 ppm

Test temperature:

21 °C

pH:

7.8 to 8.3

Dissolved oxygen:

8.6 to 8.7 mg/l

Salinity:

no data

Conductivity:

50 µmhos/cm

Nominal and measured concentrations:

100, 180, 320, 560 and 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Material, size, headspace, fill volume: 250 ml beakers containing 200 ml of well water
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates):2

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: 48h

OTHER TEST CONDITIONS
- Photoperiod: 16/8

RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

250 mg/L

Nominal / measured:

estimated

Basis for effect:

mortality

Remarks on result:

other:

Remarks:

95% C.I. 210-290 mg/l

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality

Effect concentrations 3

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

310 mg/L

Nominal / measured:

estimated

Basis for effect:

mortality

Remarks on result:

other:

Remarks:

95% C.I. 270-350 mg/l

Details on results:

See tables 1 and 2 in "any other information on results" and "overall remarks attachments" sections

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

LC50 values and 95% confidence intervals were obtained by employing a computerized LC50 program developed by Stephan et. al. 1978 (A computer program for calculating an LC50 U. S. Environ­mental Protection Agency, Duluth, Minnesota, pre-publication manu­script, August, 1978.), performing the following statistical tests: binomial, moving average and probit tests. The results from the moving average or probit tests were used when there were two or more partial mortalities. If there weren't more than one partial mortality, the results from the binomial test were recorded.

Any other information on results incl. tables

Table 1. Raw data range-finding test

Test Conc. mg/l (ppm)	24h		48h
	Dead	Observation	Dead	Observation
Control (a)	0	N	0	N
1	0	N	0	N
10	0	N	0	N
100	0	N	0	N
N= normal; LOE= loss of equilibrium; SUR= surfacing

Table 2. Raw data definitive test

Test Conc. mg/l (ppm)	24h		48h
	Dead	Observation	Dead	Observation
Control (a)	0	N	0	N
Control (b)	0	N	0	N
100 (a)	0	N	0	N
100 (b)	0	N	0	9N, 1 SUR
180 (a)	0	N	1	9N
180 (b)	1	9N	3	7N
320 (a)	4	4N, 2 SUR	8	2N
320 (b)	6	4N	7	3N
560 (a)	10		10
560 (b)	10		10
1000 (a)	10		10
1000 (b)	10		10
N= normal; LOE= loss of equilibrium; Sur= surfacing

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test item to the freshwater Daphnia magna resulted in an estimated 48-hour LC50 value of 250 mg/L. The NOEC for Propionitrile was 100 mg/l after 48 hours.

Executive summary:

The acute toxicity of Propionitrile to Daphnia magna was assessed using the methods outlined by the Commitee on Methods for Toxicity Tests with Aquatic Organisms which follows the guidelines of the OECD 202 standard for acute Daphnia tests. The study is reliable with restriction (Klimisch 2) and with GLP certification. Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination of the test and were within acceptable limits; however, no analytical measurement of test material concentrations were performed. Test concentrations of a nominal 100, 180, 320, 560 and 1000mg/l were tested. The estimated 48-hour LC50 (95% C. I.) for propionitrile was 250 mg/1 (210 -290 mg/1) and a 48 hour NOEC of 100mg/l.