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EC number: 257-297-7 | CAS number: 51581-32-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-02-23 to 1999-08-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- adopted 24 February, 1987
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 3-pyridyl dimethylcarbamate
- EC Number:
- 257-297-7
- EC Name:
- 3-pyridyl dimethylcarbamate
- Cas Number:
- 51581-32-9
- Molecular formula:
- C8H10N2O2
- IUPAC Name:
- pyridin-3-yl N,N-dimethylcarbamate
Constituent 1
- Specific details on test material used for the study:
- - CAS No.: 51581-32-9
- Batch No.: TMFP 304
- Purity: at least 99 %
- Solubility in water: Soluble
- Storage: In the refrigerator, in the dark
- Date of expiry: December 1999
- Appearance: Colorless liquid
- pH: 7.29
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Forschungsinstitut für Versuchstierzucht, A-2325 Himberg
- Age at study initiation: 8 weeks (males) and 12 weeks (females)
- Weight at study initiation: approximately 161 - 164g for males and 256 g for females
- Housing: Single caging
- Diet (e.g. ad libitum): Yes
- Water (e.g. ad libitum): Yes
- Acclimation period: 5 days (females) and 12 days (males)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22
- Humidity (%): 49
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 10 mL per kg body weight
- Doses:
- Females: 15, 50, 150 mg / kg bw
Males: 50 mg / kg bw - No. of animals per sex per dose:
- 5 per dose group. 3 dose groups for females and one for males
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: within the periods 0 - 0.5, 0.5 - 1, 1 - 2, 2 - 4 and 4 - 6 hours after administration and then at least once a day for a total of two weeks
- Frequency of weighing: Before administration, 7 days p.a, 14 days p.a., the day of death from animals died between days 1 - 14. Body weight gain was calculated for each week of the study, i.e. between 0 and 7 d p.a., 7 and 14 d p.a.
- Necropsy of survivors performed: yes - Statistics:
- Statistical methods including Bias control, LD50 and the upper and lower limits of confidence (95%) were calculated
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 99.4 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Females: All animals survived until the scheduled termination of the study at 15 and 50 mg / kg bw. 4 / 5 animals died on the day of administration at 150 mg / kg body weight
Males: All animals survived until the scheduled termination of the study at 50 mg / kg bw - Clinical signs:
- - Autonomous nervous effects: Increased salivation in mid and high dosed females
- Central nervous effects: Sedation in females and 4 / 5 males, abnormal posture in two mid dosed and in all high dosed females, tremor in two low dosed females and in all females and males of the other groups, disturbed locomotion in 4 / 5 males, clonic convulsions in 4 / 5 females of the high dosed group and righting reflex catalepsy in all mid and high dosed females and in one male.
- Signs of pain or of reduced well-being: Piloerection in all animals, hunched posture in nearly all animals, sunken flanks in one mid dosed and two high dosed females and chromodakryorrhea in one high dosed female and two males - Body weight:
- males and females: Body weights and body weight gain were inconspicuous in all animals
- Gross pathology:
- No abnormal findings were made post mortem
- Other findings:
- - Other effects: Dyspnoea in high dosed females, respiratory murmur and oedema of the skull in one high dosed female each, exophthalmus in one high dosed female and in 4 / 5 males and a corneal lesion in one high dosed female.
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- In this study, the test substance is considered toxic under the UN GHS Criteria "Category III"
- Executive summary:
In an acute oral toxicity study, the test item, diluted with deionised water was administered orally to male and female Sprague Dawley rats at a dose volume of 10 mL kg body weight at concentrations of 15, 50 and 150 mg / kg bw for males and 50 mg / kg bw for females. The LD50 for females was determined to be 99.4 mg / kg body weight. The test substance caused effects on the autonomous (e.g. salivation) and the central nervous system and a cardiovascular disorder (oedema). The signs indicate a cholinesterase-inhibiting effect, which is also likely due to the chemical nature of the test substance, but also an anaphylactoid reaction (skull oedema), with one eye lesion as a secondary effect. The cause of death may have been a direct sequel of the alterations noted in life. Based on these results, the test item is considered to be toxic under the UN GHS Criteria "Category III."
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