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Diss Factsheets
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EC number: 204-479-9 | CAS number: 121-54-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August - October 2001
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- TEST MATERIAL
- Identity: LZ1442.3, Benzethonium chloride
- Chemical name: Benzethonium chloride
- Appearance: White powder
- Storage conditions: Room temperature
- Stability under test conditions: stable for min. 3 years from manufacturing date
- Batch No.: OC0509
- Expiry date: 7 June2002
- Purity: Responsibility of sponsor
- Date received: 20 August 2001
- Safety precautions: Routine safety precautions (lab coat, gloves, safety glasses, face mask) for unknown materials were applied to assure personnel health and safety. - Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- A sample of activated sludge was collected from Eye sewage treatment works, which treats predominantly domestic waste. Aliquots (25 ml) of a homogenised sample were filtered through dried (approximately 105°C) and pre-weighed Whatman GFC filter papers. The filters were dried for
at least one hour, allowed to cool and re-weighed. The solids level in the sludge was determined and then an appropriate volume used to inoculate control and test vessels to give a final suspended solids concentration of 30 mg/1. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 5 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral salts medium
- Solubilising agent (type and concentration if used):
- Test temperature:none
- pH: The pH of all test and control mixtures was 7.6 at the start of the test and ranged between 7.4 and 7.5 at the end.
- pH adjusted: yes; and adjusted, as required, with 5N HCl to 7.4 ± 0.2. Each vessel was then fitted with a stopper holding an air inlet tube reaching approximately 10 cm, below the liquid surface and an air outlet just below the stopped:
- Suspended solids concentration: 30 mg/L
- Continuous darkness: no
TEST SYSTEM
- Culturing apparatus: 5 l amber glass bottles
- Number of culture flasks/concentration: six
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: three Dreschel bottles in series, each containing 0.025N, nominal barium hydroxide (100 ml). - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- (AR grade, product number S/2441/53) was obtained from Fisher Scientific UK.
- Preliminary study:
- no pretest performed
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 2 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 9 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- There was no evidence of biodegradation (0% degradation) of Benzethoniumchloride by the end of the test on Day 29.
- Remarks on result:
- not measured/tested
- Results with reference substance:
- The results obtained for the degradation of sodium benzoate (70% of its TC02 after 7 days and 95%after 29 days) and for cumulative C02 production by the control mixtures (55.6 and 58.3 mgC02) fulfil the validity criteria for this test.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- There was no evidence of biodegradation (0%) of Benzethoniumchloride by the end of the test on Day 29.
- Executive summary:
The objective of this study was to assess the biotic degradability of Benzethoniumchloride. It was conducted in accordance with Procedure C.4-C "C02 Evolution test" of the Annex to Directive 92/69/EEC (OECD 301B). In order to allow an assessment of ready biodegradability using this test method, a test substance concentration of 5 mg as Carbon/l was employed. This level was lower than the guideline recommendation of 10- 20 mgC/l because Benzethoniumchloride was expected to have adversely affected the activity of the microbial inoculum at a higher concentration owing to its antimicrobial properties. The degradable reference substance sodium benzoate was also employed at 5 mgC/l in order to demonstrate that the precision of the assay had not been affected by the use of a lower test substance concentration.
There was no evidence of biodegradation of Benzethoniumchloride by the end of the test on Day 29. Sodium benzoate had been biodegraded by 70% after 7 days and 95% after 29 days in the absence of Benzethoniumchloride, and by 60% after 5 days in its presence which confirmed that the test item was not inhibitory to the activity of the microbial inoculum.
Reference
Description of key information
The biotic degradability test of Benzethoniumchloride was conducted in accordance with EU testing method C.4 -C "C02 Evolution test" and OECD no. 301B. There was no evidence of biodegradation of Benzethoniumchloride by the end of the test on Day 29. The test item was not inhibitory to the activity of the microbial inoculum.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Source: GLP report
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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