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EC number: 945-924-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Nov - 20 Nov 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt Hildesheim, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control, 3.75, 7.50, 15.0, 30.0, 60.0 mg/L
- Sampling method: Samples of each concentration were taken at the beginning and at the end of the study.
- Sample storage conditions before analysis: If necessary the samples were stored at 6 ± 2 °C until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Appropriate amounts of the test substance were added directly to the test vessels. The test media were stirred for 1 min at 17000 rpm with an ultra turrax.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Method of breeding: Holding was performed at the test facility at 23 ± 2 °C and diffuse light. The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value. Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine. Food was provided three times a week at an amount of 4% of the fish body weight per feeding day. Fish were not fed 24 h prior to test start (Sera vipan, Sera GmbH, Heinsberg, Germany).
- Feeding during test: none
ACCLIMATION
- Acclimation period: at least 12 d
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): < 5% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 66 mg CaCO3/L
- Test temperature:
- 22.3 - 23.0 °C
- pH:
- 7.04 - 7.63
- Dissolved oxygen:
- 94 - 100% oxygen saturation
- Nominal and measured concentrations:
- nominal: Control, 3.75, 7.50, 15.0, 30.0, 60.0 mg/L
measured 0 h: < LOQ, 3.12, 6.95, 14.3, 27.5, 54.8 mg/L
measured 96 h: < LOQ, 2.53, 6.17, 12.9, 24.5, 51.9 mg/L (last concentration analysed on 17 Nov 2015 due to 100% mortality) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 64 L, fill volume: 40 L, covered with a floating lid
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.03 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: same as test
- Intervals of water quality measurement: measured at the start and every 24 h during test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 8.5 h light / 15.5 h dark
- Light intensity: 0.1 - 10 µmol photons *m-2*s-1 (approx. 7-750 lux)
EFFECT PARAMETERS MEASURED
- Mortality and behavioural changes were observed after 2, 24, 48, 72 and 96 h
- Size and weight was determined at the end of the study.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 6 and 60 mg/L; testing the higher concentration was planned as a definitve test using the threshold approach.
- Results used to determine the conditions for the definitive study: At 6 mg/L no mortality was recorded whereas 100% mortality was recorded at 60 mg/L after 24 h. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 42.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CI: 30 - 60 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 60 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: At 15 mg/L all fish missed the escape reflex. At 30 mg/L four fish were on lateral position, three fish were staggering and seven fish hyperventilated after 96 h. At 60 mg/L all fish were staggering and hyperventilated after 2 h, followed by exitus lethalis after 24 h.
- Observations on body length and weight: Average body length was 2.41 cm and 0.173 g after 96 h.
- Mortality of control: 0% - Reported statistics and error estimates:
- The data obtained were inadequate for the use of non linear regression models. The highest concentration caused 100% mortality, the next lower concentration caused no mortality. The geometric mean of these two concentrations was used as LC50.
- Sublethal observations / clinical signs:
Table 1: Cumulative mortality [%]
Nominal test concentration [mg/L]
Cumulative mortality [%]
2 h
24 h
48 h
72 h
96 h
60
0
100
100
100
100
30
0
0
0
0
0
15
0
0
0
0
0
7.5
0
0
0
0
0
3.75
0
0
0
0
0
Control
0
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study resulted in a LC50 (96 h) of 42.4 mg/L based on mortality of D. rerio (OECD 203, GLP).
Reference
Description of key information
LC50 (96 h): 42.4 mg/L based on mortality of D. rerio (OECD 203).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 42.4 mg/L
Additional information
One experimental study is available investigating the toxicity of the substance to freshwater fish. The study was conducted according to OECD 203 and GLP under static conditions with Danio rerio as test species. Prior to the definitive test the threshold approach was followed by conducting a limit test with a concentration of 60 mg/L. Since all fish died after 2 h of exposure an additional range-finding test followed by the definitive test with concentrations of 3.75, 7.50, 15.0, 30.0, 60.0 mg/L were conducted. The test concentrations were analysed by HPLC-DAD at the beginning and the end of exposure. Since they remained within 80-120% of nominal for the relevant concentrations the effect concentrations were based on the nominal values. After 96 h 100% mortality was observed at 60 mg/L whereas 0% mortality was recorded in the remaining concentrations. The LC50 (96 h) was calculated at 42.4 mg/L (nominal). The LC50 was calculated using the geometric mean of 30 and 60 mg/L since no non-linear regression methods could be used.
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