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EC number: 914-460-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-11-19 - 2009-01-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to relevant guidelines and compliant to GLP. Results are plauslible and well reported.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- GLP according to German Chemikaliengesetz and Directive 88/320/EEC
Test material
- Reference substance name:
- Reaction mass of Fatty acids, montan-wax and Fatty acids, montan-wax, 1-methyl-1,3-propanediyl esters and Fatty acids, montan-wax, calcium salts and Montan wax
- EC Number:
- 914-460-3
- IUPAC Name:
- Reaction mass of Fatty acids, montan-wax and Fatty acids, montan-wax, 1-methyl-1,3-propanediyl esters and Fatty acids, montan-wax, calcium salts and Montan wax
- Details on test material:
- TEST ITEM: Licowax OP
Chemical characterisation: Fatty acids, montan wax, 1-methyl-1,3-propanediylesters
Appearance: Pale yellow flakes
Solubility in water: Not specified
Recommended storage: Cool and dry
Storage at test facility: 6 ± 2 °C, protected from moisture and light
Retention of test item: At least 1 g has been retained
Identification parameter at test facility: Name, batch number, state, consistency and colour
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Determination of TOC-concentration at the start of the test. However, due to the very poor solubility of the test item in water the concentration was below the detection limit.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Stock solution / Dispersion treatment:
A stock solution of 100 mg/L was prepared with dilution water and agitated at 20 rpm for 48 h at room temperature. After a separation phase of 1 h the water accomodated fraction* was taken from the homogenous liquid phase.
Loading level of the water accommodated fraction*:
One loading level of 100 mg/L was tested in a limit test. Per definition of the water accomodated fraction, all terms related to concentration levels will be given as loading levels as partly dissolved compounds and mixtures cannot be related to concentrations.
*) Water accommodated fractions: The aqueous medium containing only the fraction of a multi-component test item that is dissolved and / or present as a stable dispersion/emulsion under the test conditions in the test medium, acc. to OECD Series, No. 23.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Test organism: Desmodesmus subspicatus CHODAT SAG 86.81 (formerly Scenedesmus subspicatus CHODAT)
Reason for the selection of the test system: According to the guideline Desmodesmus subspicatus CHODAT SAG 86.81 is suitable for this kind of study.
Origin: Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universität Göttingen, Nikolausberger Weg 18, D-37073 Göttingen
Cultivation at test facility: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted 35-70 µE * mE(-2)*sE(-1) for 24 h per day.
Culture medium: Nutrient medium Z according to LÜTTGE et al. (1994).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- 0.24 mmol/L (Ca2+ and Mg2+)
- Test temperature:
- minimum 21°C, mean 22.5°C maximum 24°C
- pH:
- treatment:
Start: 8.13 End: 8.39
Control:
Start: 7.83 End 8.53 - Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- As the solubility of the test item is very poor, a water accomodated fraction of a nominal loading of 100 mg/L had been used for a limit test.
- Details on test conditions:
- CONTROL: Six replicates (without test item) were tested under the same test conditions as the test replicates.
TREATMENT: Six replicates
SELF FLUORESCENCE: No self fluorescence has been found up to the water accommodated fraction of 100 mg/L.
REFERENCE ITEM Potassium dichromate p.a. (Sigma), CAS No. 7778 - 50 - 9
Purity: >= 99.5 %
Batch No.: 06719MH
Test concentrations: 0.28 - 0.51 - 0.90 - 1.6 mg/L
Experimental date: September 22 to 25th, 2008
TEST METHOD:
Static procedure
Exposure duration: 72 h
Replicates: Six replicates per limit concentration and control
Test container: Sterile Erlenmeyer flasks, volume: 250 mL
Test volume: 100 mL
Dilution water: OECD-medium according to the guideline
Preculture: A three day old preculture was used as inoculum. Incubation was performed in 500 mL Erlenmeyer flasks with dilution water. For the start of the test the preculture was directly pipetted to the test and control replicates to receive an initial cell concentration of approximately 2 ¿ 5 x 10E(3) cells/mL in the replicates.
Application: Application was carried out by adding appropriate volumes of the water accommodated fraction to the test replicates.
Temperature: Nominal range: 21 ¿ 24 °C, controlled at ± 2 °C
Agitation: Test containers were placed on a rotary shaker and oscillated at approximately 70 rpm.
Light intensity: 60 - 120 µE * mE(-2) * sE(-1)
Light regime: 24 h/d light
TYPE AND FREQUENCY OF MEASUREMENT
Cell density was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal. The pH-value at the beginning of the test was measured for one additional replicate of the water accommodated fraction and the control. At the end it was measured from one pool of all replicates of the water accommodated fraction and the control. The room temperature was measured continuously by a hygro-thermograph. Light intensity was measured prior to test start. Microscopic evaluation of the cells at the start and end of the incubation were carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of alga cells. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Due to poor solubility of the test item, a water accomodated frachtion at nominal 100 mg/L had been used for a limit test
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Due to poor solubility of the test item, a water accomodated frachtion at nominal 100 mg/L had been used for a limit test
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Due to poor solubility of the test item, a water accomodated frachtion at nominal 100 mg/L had been used for a limit test
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Due to poor solubility of the test item, a water accomodated frachtion at nominal 100 mg/L had been used for a limit test
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Due to poor solubility of the test item, a water accomodated frachtion at nominal 100 mg/L had been used for a limit test
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Due to poor solubility of the test item, a water accomodated frachtion at nominal 100 mg/L had been used for a limit test
- Details on results:
- Microscopic evaluation of the cells at start and end of the incubation period revealed no morphological abnormalities. Environmental conditions, pH-value (measured at 0 and 72 h) and room temperature (measured continuously) were determined to be within the acceptable limits.
- Results with reference substance (positive control):
- The toxicity of potassium dichromate to the unicellular freshwater green alga Desmodesmus subspicatus was determined over a period of 72 h from September 22 to 25, 2008. The reference item toxicity is in the valid range:
EC50 (biomass, 72h) = 0.69 mg/L (95% CL 0.64-0.74)
EC50 (growth rate, 72h) = 0.31 mg/L (95% CL 0.30-0.32) - Reported statistics and error estimates:
- Not applicable
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a 72h growth inhibition study on the unicellular alga Desmodesmus subspicatus (reliability category 1, compliant to GLP), the test item showed no inhibitory effect at the nominal test concentration of 100 mg/L (water accomodated fraction, limit test): EC50 (72h, growth rate and biomass) > 100 mg/L nominal concentration.
The results with the tested reference substance potassium dichromate proove the sensitivity of the applied test system. - Executive summary:
The toxicity of Licowax OP to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 at DR.U.NOACK-LABORATORIEN in 31157 Sarstedt, Germany from January 12 to 15, 2009. The aim of the study was to assess the effects on growth rate and biomass over a period of 72 h in a limit test. The study was conducted under static conditions with an initial cell density of approximately 2 - 5 x 10E(3) cells/mL. A water accommodated fraction* of 100 mg/L was prepared. Per definition of the water accommodated fraction all terms related to concentration levels will be given as loading levels because partly dissolved compounds and mixtures cannot be related to concentrations. Six replicates were tested for the water accommodated fraction and the control. Environmental conditions were determined to be within the acceptable limits. The concentration of Licowax OP was analysed at test start via TOC-analysis. Due to the poor solubility of the test item the actual concentration was below the detection limit. All effect levels are given based on the water accomodated fraction of the test item:
EC10 (72h, growth rate and biomass) > 100 mg/L
EC20 (72h, growth rate and biomass) > 100 mg/L
EC50 (72h, growth rate and biomass) > 100 mg/L
*) Water accommodated fractions: The aqueous medium containing only the fraction of a multi-component test item that is dissolved and / or present as a stable dispersion/emulsion under the test conditions in the test medium, acc. to OECD Series, No. 23.
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