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EC number: 200-562-9 | CAS number: 63-68-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Assessment of the performance of the Ames II™ assay: a collaborative study with 19 coded compounds
- Author:
- S Flückiger-Isler, M Baumeister, K Braun, V Gervais, N Hasler-Nguyen,eR Reimann,J Van Gompel, H.-G Wunderlich,G Engelhardt
- Year:
- 2 004
- Bibliographic source:
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis Volume 558, Issues 1–2, Pages 181–197
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Assessment of mutagenicity using the Ames II assay. The Ames II assay is a is a liquid microtiter modification of the Ames test and consists of the ‘strains’ TAMix and TA98. TAMix is a mixture of the Salmonella typhimurium strains TA7001, TA7002, TA7003, TA7004, TA7005 and TA7006.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- L-methionine
- EC Number:
- 200-562-9
- EC Name:
- L-methionine
- Cas Number:
- 63-68-3
- Molecular formula:
- C5H11NO2S
- IUPAC Name:
- L-methionine
- Details on test material:
- - Name of test material (as cited in study report): L-Methionine
- Analytical purity: 98%
- Other: Supplier: Sigma
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- other: TAMix
- Details on mammalian cell type (if applicable):
- TAMix consists of the strains TA7001, TA7002, TA7003, TA7004, TA7005, and TA7006.
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9
- Test concentrations with justification for top dose:
- Six concentrations, plus a negative (solvent) control and a positive control were tested each culture was treated and dispensed into microtiter plates in triplicate. Compounds were tested without any determination for viability or optimization for dose. The highest and the lowest dose level were 5000 and 4g/ml, respectively, and the intermediate doses were spaced at two- to five-fold intervals.
One group used its own internally validated setup for an automated system which differed from the prescribed protocol in that a 10
times lower top dose (500 g/ml) was used, the triplicate values derived from three different overnight cultures, there was no agitation during the 90 min of exposure, and the plate scoring was performed through spectrophotometry. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO (final concentration of 4% in the assay)
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 2-nitrofluorene
- other: 2-Aminoanthracene
- Details on test system and experimental conditions:
- without S9 mix:
Into 1-well of a 24-well plate (one well/strain/dose/replicate), 0.215 ml of Exposure Medium and 0.025 ml of culture were aliquoted resulting in a total volume of 0.240 ml.
with S9 mix:
Into 1-well of a 24-well plate (one well/strain/dose/replicate), 0.1775 ml of Exposure Medium, 0.025 ml of culture and 0.0375 ml of 30% S9 mix were aliquoted resulting in a total volume of 0.240 ml.
To each of these cultures, 0.01 ml of test chemical, was added, to give a final volume of 0.250 ml. This mixture was incubated for 90 min at 37°C with agitation at 250 rpm. After 90-min incubation, each well of the 24-well plates containing the chemically treated cultures received 2.8 ml of Indicator Medium. The cultures were mixed gently with the histidine-deficient Indicator Medium that selects for prototrophic reversion before being distributed in 0.05 ml aliquots to 48 wells of a 384-well microtiter plate. One plate was used per strain and replicate. The plates were incubated at 37°C for 48 h. An essential constitution of the Indicator Medium (Bromocresol purple), turns yellow as the pH drops due to the catabolic activity of revertant cells which grow in the absence of histidine.
The number of positive (yellow) wells out of 48 wells per replicate and dose was compared with the number of spontaneous revertants obtained in the negative control section. The average number of wells containing revertants per culture and concentration was calculated from the triplicate sections, and the increases above the zero dose were determined at each concentration of the test chemicals. - Evaluation criteria:
- Revertant yields greater than two times the baseline level obtained in the triplicate values of a given dose was classified as an increase in revertant yield of the assay. Multiple responses of greater than two-fold the baseline level led to the classification as a clear positive.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Species / strain:
- other: TAMix
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
L-Methionine was tested in the Ames II assay in four laboratories. Non of the laboratories obtained a mutagenic effect with methionine.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
L-Methionine was tested in the Ames II assay in four laboratories. Non of the laboratories obtained a mutagenic effect with L-methionine.
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