3-methylbut-2-en-1-ol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

reduced post observation time (7 days instead of 14 days). No analytical verification of test substance concentration applied.

Principles of method if other than guideline:

The inhalation hazard test demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of a test substance at the temperature chosen for vapor generation ( 20°C). Several groups of 3 rats per sex were sequentially exposed to the vapors, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted disc in a glass cylinder for different time periods.
No analytical determination of the atmosphere concentrations was performed. The nominal concentration was calculated as quotient of the amount of the test substance weight loss during exposure. Group-wise documentation of clinical signs was performed over the 14 day study period. Body weight of groups was determined before the start of the study and at the end of the observation period in surviving animals.

GLP compliance:

no

Remarks:

GLP was not compulsory at the time the study was conducted.

Test type:

other: Inhalation Hazard Test (IHT)

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-2-en-1-ol

EC Number:

209-141-4

EC Name:

3-methylbut-2-en-1-ol

Cas Number:

556-82-1

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-2-en-1-ol

Details on test material:

- Name of test substance: 3-Methylbut-2-en-1-ol (Prenol)
- Analytical purity: 95 %
- Impurities: no data given

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Average weight at study initiation: 200 - 250 g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: The animals were exposed to atmosphere saturated with the volatile parts of the test material in the air at 20 °C.

Details on inhalation exposure:

Saturation of the atmosphere was generated by conducting an air stream through a substance layer of 5 cm height.
The rats were exposed to the test atmosphere for 3 and 8 hours.
Air flow was 200 l/h in the exposure chamber.

Analytical verification of test atmosphere concentrations:

no

Remarks on duration:

3 or 8 hours

Concentrations:

Nominal concentrations of the test substance in the atmosphere were calculated to be 10.25 mg/l (3 hrs) and 8.4 mg/l (8 hrs) by using the weight loss of test substance and the amount of air used during the exposure. 

No. of animals per sex per dose:

6 males and 6 females (3 hours),
3 males and 3 females (8 hours).

Control animals:

no

Details on study design:

Animals were inspected for signs of pharmacologic or toxicologic effects during a 7 days post observation period. Body weight was measured before dosing. At the end of the observation period survivors were sacrificed and necropsied as were animals that died.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

other: IHT

Effect level:

10.25 mg/L air

Exp. duration:

3 h

Remarks on result:

other: No mortality

Effect levels 2

Sex:

male/female

Dose descriptor:

other: IHT

Effect level:

8.4 mg/L air

Exp. duration:

8 h

Remarks on result:

other: 1/6 animals died

Mortality:

No mortality was observed when 12 rats were exposed for 3 hours 
Mortality in 1 animal out of 6 occurred after exposure of 8 hours during the first 24 hrs.

Clinical signs:

other: Attempt to escape, strong secretion out of the eyes and the nose, after the exposure tremor.

Body weight:

The average body weight gains were within the normal limits.

Gross pathology:

In the animal that died, multifocal congestion of the lungs was seen, all other animals were without abnormalities.