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Diss Factsheets
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EC number: 609-870-1 | CAS number: 40835-18-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study, not deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- methyl (2E)-3-methyl-4-oxobut-2-enoate
- EC Number:
- 609-870-1
- Cas Number:
- 40835-18-5
- Molecular formula:
- C6 H8 O3
- IUPAC Name:
- methyl (2E)-3-methyl-4-oxobut-2-enoate
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): 3-Formylcrotonsäuremethylester
- Physical state: liquid
- Analytical purity: 99.2 %
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components:
- Lot/batch No.: Abl. Nr. 00-0007
- Storage condition of test material: ambient
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for analytical determination of the concentration were taken representatively
from each concentration group at start and end of the first and the last interval. Water
samples were taken from the middle of the test vessel using a glass pipette and were
transported to the analytical laboratory in glass ampoules, which were rinsed with test
solution before they were filled with exact volumina.
The transport to the Analytical Laboratory was done on the day of sampling.
For the dates of analysis and storage of samples if applicable see Analytical report in the
Appendix.
The sampels drawn at end of exposure were stored in the freezer for 3 days due to
technical reasons.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test concentrations were freshly prepared daily separately for each test vessel . The test substance was weighed into a beaker. The defined volume of test water was filled into the test vessel . An aliquot from the test water in the test vessel was taken and mixed with the test substance using an ultra turrax and the mixture was poured into the test vessel and the whole test batch was homogenized again with an ultra turrax.
The test concentrations were prepared as follows: 50, 100, 220, 500 and 1000 g, respecively in 10 L water each.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: Zierfisch-Center Klöckner, Ludwigshafen
- Age at study initiation (mean and range, SD): approx. 5 month
- Length at study initiation (length definition, mean, range and SD): 0.65 g (0.40 - 1.19 g)
- Weight at study initiation (mean and range, SD): 4.0 cm (3.6 - 4.7 cm)
- Feeding during test: no
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Commercial fish diet „Tetramin", supplier Tetra-Werke, Melle, Germany, ad libitum, additionally generally on workdays live brine shrimp (artemia).
- Health during acclimation (any mortality observed):
No signs for sickness, injuries or abnormalities were observed .
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 2.5 mmol/L = 250 mg/L CaCO3
- Test temperature:
- 22 °C
- pH:
- 7.7 - 8.4
- Dissolved oxygen:
- 5.6 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 5, 10, 22, 50, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Reported statistics and error estimates:
- The median lethal concentration (LC50) after 1, 4, 24, 48, 72 and 96 hours based on the nominal concentrations and based on the mean of the analytically determined concentrations was calculated using probit analysis* . If possible the 95% confidence intervals were given as well .
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.