Isooctyl mercaptoacetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Isooctyl mercaptoacetate

EC Number:

246-613-9

EC Name:

Isooctyl mercaptoacetate

Cas Number:

25103-09-7

Molecular formula:

C10H20O2S

IUPAC Name:

2-methylheptyl 2-sulfanylacetate

Details on test material:

Identification: Isooctyl thioglycolate (IOTG)
CAS no. 25103-09-7
Source: Elf Aquitaine production
Batch: EAP 94-000739
Purity: 99.05 % (w/w)

Method

Target gene:

Histidine reversion

Species / strain

Species / strain / cell type:

S. typhimurium, other: Strains TA98, TA100, TA102, TA1535 & TA1537

Metabolic activation:

with and without

Metabolic activation system:

liver microsomal fraction (S9 fraction) of rats induced with Aroclor 1254

Test concentrations with justification for top dose:

. Without S9: 
First test:       
TA102: 200, 400, 800, 1600, 3200 µg/plate      
TA98 and TA1537: 50, 100, 200, 400, 800 µg/plate      
TA100 and TA1535: 25, 50, 100, 200, 400 µg/plate
Second test:      
TA98, TA1535 and TA1537: 3.125, 6.25, 12.5, 25, 50 µg/plate      
TA100: 12.5, 25, 50, 100, 150 µg/plate      
TA102: 500, 1000, 2000, 3000, 4000 µg/plate
. With S9: 
First test :  321.5, 625, 1250, 2500, 5000 µg/plate (all strains)
Second test : 125, 250, 500, 1000, 2000 µg/plate (all strains)
Third test: 31.25, 62.5, 125, 250, 500 µg/plate (all strains)

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: see below

Results and discussion

Test results

Species / strain:

S. typhimurium, other: Strains TA98, TA100, TA102, TA1535 & TA1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

All top doses showed moderate or marked toxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

IOTG did not induce mutagenicity in Salmonella bacterial strains with or without metabolic activation.

Executive summary:

IOTG was negative for mutagenicity in a standard bacterial mutagenicity test with and without metabolic activation. This study followed OECD test guideline 471. Five strains of bacteria Salmonella typhimurium: TA 1535, TA 1537, TA 98, TA 100 and TA 102 were used. Each strain was exposed to five dose-levels of the test item (three plates/dose level). After 48 to 72 hours of incubation at 37°C, the revertant colonies were scored. In in vitro bacterial mutation assays IOTG did not induce mutagenicity in Salmonella bacterial strains with or without metabolic activation. The positive controls responded as expected.