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EC number: 212-742-4 | CAS number: 865-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Epidemiological data
Administrative data
- Endpoint:
- epidemiological data
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- The association of waterborne chloroform with intrauterine growth retardation
- Author:
- Kramer MD, Lynch CF, Isacson P, Hanson JW
- Year:
- 1 992
- Bibliographic source:
- Epidemiology 3(5), 407-413
Materials and methods
- Study type:
- case control study (retrospective)
- Endpoint addressed:
- developmental toxicity / teratogenicity
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Population-based case control analysis on the association of chloroform in drinking water with low birth weight, prematurity, and intrauterine growth retardation applying multiple logistic regression statistics.
- GLP compliance:
- no
Test material
- Reference substance name:
- Chloroform
- EC Number:
- 200-663-8
- EC Name:
- Chloroform
- Cas Number:
- 67-66-3
- Molecular formula:
- CHCl3
- IUPAC Name:
- chloroform
- Test material form:
- liquid
Constituent 1
Method
- Type of population:
- general
- Ethical approval:
- not specified
- Details on study design:
- HYPOTHESIS TESTED (if cohort or case control study): The study was conducted to determine whether water supplies containing relatively high levels of chloroform and other trihalomethanes within the state of Iowa are associated with low birth weight, prematurity, or intrauterine growth retardation.METHOD OF DATA COLLECTION- Type: statistical analysis of birth certificates and water quality survey data- Details: STUDY PERIOD: January 1, 1989 to June 30, 1990SETTING: Iowa towns with 1000 to 5000 inhabitantsSTUDY POPULATION- Total population (Total no. of persons in cohort from which the subjects were drawn): no data- Selection criteria: singleton, live infants exhibiting low birth weight, prematurity, or intrauterine growth retardation born to non-Hispanic white women 19 years of age or older and who were residents of Iowa towns with 1000-5000 inhabitants that derived 100 % of their public drinking water from a single source- Total number of subjects participating in study: 159 live singleton infants weighing < 2500 g (795 randomly selected control infants weighing > 2500 g), 342 singleton, live infants with gestational ages < 37 weeks (1710 randomly selected control infants with gestational ages > 37 weeks, 187 growth-retarded singleton, live infants (935 randomly selected control infants)- Sex/age/race: no data/new born/non-Hispanic, whiteCOMPARISON POPULATION- Randomly selected healthy infants HEALTH EFFECTS STUDIED- Low birth weight, prematurity, intrauterine growth retardation
- Exposure assessment:
- estimated
- Details on exposure:
- Exposure status was assigned to infants according to maternal residence in a given municipality at the time of birth. The chloroform levels in drinking water were based on a 1987 municipal water survey which included standardised analyses of finished water samples for four trihalomethanes: chloroform, dichlorobromomethane, dibromochloromethane and bromoform. For chloroform, the exposure levels were categorised as undetectable, low (1-9 microgram/litre) and high (> 10 microgram/litre).
- Statistical methods:
- Multiple logistic regression was used to estimate odds ratios and their 95 % confidence intervals. The regression model for each outcoe inluded adjustment for maternal age, parity, marital status, education, maternal smoking during pregnancy, and adequacy of prenatal care. Since levels of the four trihalomethanes are often correlated, only one trihalomethane exposure was regressed at a time to avoid multicollinearity.
Results and discussion
- Results:
- There was an increased risk of intrauterine growth retardation associated with higher concentrations of waterborne chloroform and dichlorobromomethane. The elevated risk associated with chloroform remained even when the analysis was restricted to chlorinated water sources, indicating that chloroform has an association over and above that attributable to chlorination alone.
- Strengths and weaknesses:
- The major limitation of the study involve the ascertainment and classification of exposures to trihalomethanes, including such issue as the imprecision of using aggregate municipal measures for classifying exposure at the level of the individual, potential misclassification due to residential mobility and the fluctuation of trihalomethane levels. The influence of pesticides, herbicides and nitrates could not be directly controlled because these were not measured in the municipal waer survey.
Any other information on results incl. tables
Table 1: Association of adverse reproductive outcomes with waterborne chloroform exposure.
Low birth weight |
||||
Exposure to chloroform (mg/L) |
Cases (n=159) |
Controls (n=795) |
Odds ratio* |
95 % confidence interval |
> 10 |
25 |
110 |
1.3 |
0.8-2.2 |
1-9 |
73 |
370 |
1.1 |
0.7-1.6 |
undetectable |
61 |
315 |
1.0 |
reference |
Prematurity |
||||
Exposure to chloroform (mg/L) |
Cases (n=342) |
Controls (n=1710) |
Odds ratio* |
95 % confidence interval |
> 10 |
39 |
202 |
1.1 |
0.7-1.6 |
1-9 |
63 |
786 |
1.1 |
0.8-1.4 |
undetectable |
140 |
722 |
1.0 |
reference |
Intrauterine growth retardation** |
||||
Exposure to chloroform (mg/L) |
Cases (n=342) |
Controls (n=1710) |
Odds ratio* |
95 % confidence interval |
> 10 |
32 |
113 |
1.8 |
1.1-2.9 |
1-9 |
92 |
446 |
1.3 |
0.9-1.8 |
undetectable |
63 |
376 |
1.0 |
reference |
* Adjusted for marital status, maternal age, number of previous children, prenatal care, maternal smoking and education; ** Intrauterine growth retardation is defined as being under the 5th percentile of weight for gestational age according to California data on non-Hispanic whites.
Applicant's summary and conclusion
- Conclusions:
- There was an increased risk of intrauterine growth retardation associated with higher concentrations of waterborne chloroform.
- Executive summary:
A population-based case-control analysis of the association of chloroform and other trihalomethanes in drinking water with low birth weight, prematurity and intrauterine growth retardation was performed. The analysis used Iowa birth certificates data from January 1, 1989, to June 30, 1990. The study was restricted to singleton live infants born to non-Hispanic white women 19 years of age or older who were residents of Iowa towns with 1000 -5000 inhabitants that derived 100 % of their public drinking water from a single source. Exposure was classified using data from a 1987 municipal drinking water survey measuring chloroform in a unified manner. Three exposure categories were used: undetectable chloroform, 1 -9 microgram/litre of chloroform, > 10 microgram/litre of chloroform.
159 infants exhibiting birth weights below 2500 g were compared to 795 randomly selected control infants, 342 infants exhibiting prematurity were compared to 1710 randomly selected control infants, and 187 infants with growth retardation were compared to 935 randomly selected control infants. Comparisons showed that there was an increased risk of intrauterine growth retardation associated with higher concentrations of waterborne chloroform and dichlorobromomethane. The elevated risk associated with chloroform remained even when the analysis was restricted to chlorinated water sources, indicating that chloroform has an association over and above that attributable to chlorination alone.
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