Cyclohexane, oxidized, aq. ext., sodium salt

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guidelien study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl difluoroacetate

EC Number:

207-223-4

EC Name:

Ethyl difluoroacetate

Cas Number:

454-31-9

IUPAC Name:

ethyl difluoroacetate

Constituent 2

Reference substance name:

5-Ethylpyridine-2,3-dicarboxylic Acid

IUPAC Name:

5-Ethylpyridine-2,3-dicarboxylic Acid

Details on test material:

- Name of test substance: Natriumcarboxylat- Lösung
- Test substance No.: 09/0194-1
- Batch identification: Probe EX 1672 vom 16.03.2009
- CAS No.: 68915-39-9

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Composition:

Hydroxycapronic acid 4.1 %(weight)
Valerianic acid 7.4 %(weight)
Glutaric acid 0.49 %(weight)
Capronic acid 3.3 %(weight)
Adipic acid 2.5 %(weight)
Formic acid 2.4 %(weight)
Acetic acid 1.3 %(weight)
Succinic acid 0.17 %(weight)
Propionic acid 0.45 %(weight)
Butyric acid 1.1 %(weight)
Water 53.6 %(weight)
Sodium carbonate 1.65 %(weight)
Sodium hydrogencarbonate 2.47 %(weight)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

- The test solution was prepared by dissolvation of 259.69 mg in 1L M4-medium.
- The test solution was stirred for about 10 minutes at 20 ± 2 °C to ensure complete dissolvation.
- the test solution was visibly clear and colored light yellow

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUSS
- Source: Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique appliquee, France", and has been breeded from 1978 in the Laboratories of BASF.
- Age at study initiation (mean and range, SD): 2 - 24 hours old

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/L

Test temperature:

19.8 - 21.5 °C

pH:

8.1 - 8.7

Dissolved oxygen:

8.2 - 9.2

Nominal and measured concentrations:

Nominal: 0 and 260 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable):closed
- Material, size, headspace, fill volume: 20 ml glass tubes with flat bottom, 10 ml test volume
- Aeration:
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium with the properties:
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.8 - 1.0 mmol/L
Ratio Ca/Mg ions: about 4:1
pH value: 7.5 - 8.5
Conductivity: 550 - 650 microS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light (about 2-7 microeinstein/m*m*s (400-750 nm))


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observations: mobile daphnids after 24 and 48 h
Measurements: Temperature, pH and oxygen content after 0 and 48 h

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 115 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

other: adjusted to water content

Basis for effect:

mobility

Remarks on result:

other: 248 mg/L non-adjusted t owater content adjusted to a water content of 53.6%

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 248 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

other: non-adjusted to water content

Basis for effect:

mobility

Remarks on result:

other: Test substance incl. 53.6% /weight (water)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes