Registration Dossier
Registration Dossier
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EC number: 204-970-8 | CAS number: 129-93-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 6,8-dianilinonaphthalene-1-sulphonic acid
- EC Number:
- 204-970-8
- EC Name:
- 6,8-dianilinonaphthalene-1-sulphonic acid
- Cas Number:
- 129-93-1
- Molecular formula:
- C22H18N2O3S
- IUPAC Name:
- 6,8-bis(phenylamino)naphthalene-1-sulfonic acid
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: A mixed population of activated sewage sludge microorganisms.
- Details on inoculum:
- Activated sewage was prepared by sampling 10 different sites around the UK in accordance with OECD Guideline No. 302C.
The mixed sludge was fed daily with a 0.1% synthetic sewage and maintained on a constant aeration at 25 ± 1°C.
Usage Rate: Equivalent to 100 mg dry weight/1. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- measured by direct manometer reading
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Aniline attained 63% biodegradation after 7 days and 85% after 14 days thereby confirming the suitability of the inoculum and culture conditions
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The substance is not biodegradable
- Executive summary:
DPNS attained negligible degradation after 28 days calculated from oxygen uptake (% of COD) and so, therefore, cannot be considered as inherently biodegradable under the strict terms and conditions of the OECD Guidelines.
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