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EC number: 281-866-9 | CAS number: 84045-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- None
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Principles of method if other than guideline:
- None
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonatonaphthalen-2-yl)diazenyl]naphthalene-2,7-disulfonate
- Cas Number:
- 84045-65-8
- Molecular formula:
- C31H21Na3ClN7O10S3
- IUPAC Name:
- Trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonatonaphthalen-2-yl)diazenyl]naphthalene-2,7-disulfonate
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- None
Test animals
- Species:
- rat
- Strain:
- other: Tif:RAIf (SPF)
- Remarks:
- F3-crosses of RII 1/Tif x RII 2/Tif
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Species and strain: Rat, Tif:RAIf(SPF), F3-crosses of RII 1/Tif x RII 2/Tif
Source: CIBA-GEIGY LTD. Tierfarm, 4334 Sisseln, Switzerland
Initial body weight range: 168-203 g
Initial age: 7-8 weeks
Individual identification: by colour code using picric acid
Husbandry: The animals were kept under conventional laboratory conditions. They were caged in groups of 5 in Macrolon cages type 4 with standardized soft wood bedding (Société Parisienne des sciures, Pantin).
The animal room was air conditionned: temperature 22±3° C, relative humidity 55±15%, 12 hours light/day, approximately 15 air changes/h.
Diet: Rat food, NAFAG No. 890, NAFAG AG, Gossau, SG (Switzerland), and water were provided ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: Distilled water containing 0.5% carboxymethylcellulose and 0.1% polysorbate 80.
- Details on oral exposure:
- Prior to dosing, the animals were fasted overnight.
Administration: oral, by gastric intubation (gavage)
Volume (ml/kg body weight) applied: 10 and 20 - Doses:
- 2000 and 5000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days or until all symptoms have disappeared, whichever lasts longer,
- Mortality: daily; a.m. and p.m. on working days, a.m. on weekend days,
- Signs and symptoms: daily,
- Weighing: on days 1, 7, 14 and at death,
- Necropsy of survivors performed: yes,
Spontaneously dying animals were submitted to a gross necropsy as soon as possible; survivors at the end of the observation period. - Statistics:
- From the body weights, the group means and their standard deviations were calculated.
Where feasible, the LD50 including the 95 % confidence limit were computed by the logit method (J. Berkson, J.Am. Stat. Ass. 39. 357-65, 1944).
Results and discussion
- Preliminary study:
- None
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality occurred at the dose of 2000 mg/kg bw. However, at 5000 mg/kg bw, 3 males and 5 females were found dead within day 1 after treatment.
- Clinical signs:
- other: Dyspnoea, ruffled fur, diarrhoea and curved body position were seen with the animals treated at 2000 mg/kg bw. Animals treated with 5000 mg/kg bw, also showed sedation, exophthalmos , ventral body position and lateral body position in addition to the prev
- Gross pathology:
- No gross lesions were found in the animals of the 2000 mg/kg group. With the exception of the two survivors, all animals of the 5000 mg/kg groups had blue stained bodies.
- Other findings:
- None
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test substance was determined to have a LD50 of >2000 mg/kg bw.
- Executive summary:
The acute oral toxicity of the test substance was evaluated in a study conducted according to OECD Guideline 401. Two groups of 5 male and 5 female rats received the test item at the doses of 2000 and 5000 mg/kg bw by oral intubation. Observations of mortality and clinical signs were carried out after 1, 2, 3, 5 and 24 hrs of dosing, and daily thereafter for 14 days; while weighing was done on days 1, 7 and 14. Spontaneously dying animals were submitted to a gross necropsy as soon as possible; survivors at the end of the observation period. No mortality occurred at the dose of 2000 mg/kg bw. However, at 5000 mg/kg bw, 3 males and 5 females were found dead within day 1 after treatment. Dyspnoea, ruffled fur, diarrhoea and curved body position were seen with the animals treated at 2000 mg/kg bw. Animals treated with 5000 mg/kg bw, also showed sedation, exophthalmos, ventral body position and lateral body position in addition to the previously described signs. The surviving animals recovered within 9-10 days. No effect on body weight gains was seen. No gross lesions were found in the animals of the 2000 mg/kg group. With the exception of the two survivors, all animals of the 5000 mg/kg groups had blue stained bodies. Hence, based on the above findings, the test substance was determined to have a LD50 of >2000 mg/kg bw.
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