Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 265-529-3 | CAS number: 65151-26-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Remarks:
- according to OECD 209, but limited documentation regarding the test material
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
Test material
- Reference substance name:
- Disodium 7-[(4-amino-3-methoxyphenyl)azo]naphthalene-1,3-disulphonate
- EC Number:
- 265-529-3
- EC Name:
- Disodium 7-[(4-amino-3-methoxyphenyl)azo]naphthalene-1,3-disulphonate
- Cas Number:
- 65151-26-0
- Molecular formula:
- C17H15N3O7S2.2Na
- IUPAC Name:
- disodium 7-[(4-amino-3-methoxyphenyl)diazenyl]naphthalene-1,3-disulfonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION OF TEST SOLUTION
The test item was weighed out directly into the test vessels.
The following nominal concentrations of AGAMA Base were chosen: 125, 250, 500, 1000 and 2000 mg/L.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Inoculum: activated sludge from the aeration tank of a municipal biological waste water treatment plant, not adapted and not pre-conditioned
- Name and location of sewage treatment plant where inoculum was collected: ARA Werdhoelzli, 8048 Zuerich, Switzerland
- Concentration: 1.6 g/L dry matter in the final mixture
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 22 ± 0.5°C
- pH:
- 7.5 ± 0.5
- Nominal and measured concentrations:
- Nominal concentrations: 125 mg/L, 250 mg/L, 500 mg/L, 1000 mg/L, 2000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- 1000 mL flasks
- fill volume: 500 mL
- Aeration: aerated by shaking 120 rpm
- No. of vessels per concentration (replicates): 1 (5 concentrations)
- No. of vessels per blank control (replicates): 2
- No. of vessels per reference substance control: 1 replicate sample (3 test concentrations)
TEST MEDIUM
The activated sludge was used immediately after sampling from the treatment plant without adaptation. Prior to the test, the sludge was washed twice with tap water. The test vessels contained 500 mL of final test medium anc activated sludge in an amount corresponding to 1.6 g/L dry matter in the final mixture.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- the pH-value of the activated sludge was measured prior to testing and adjusted to pH 7.5 ± 0.5, if necessary
- the oxygen concentration was measured with a oxygen electrode (ORION 9708) inserted to a 300 mL BOD bottle and recorded for about 5 to 10 min
TEST CONCENTRATIONS
- Range finding study not conducted.
- Test concentrations: 125 mg/L, 250 mg/L, 500 mg/L, 1000 mg/L, 2000 mg/L (nominal)
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 1 450 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- the EC50-value for the reference substance 3,5-dichlorophenol was estimated to be 2.5 mg/L which was low but in the range of 5 mg/L thus confirming suitability of activated sludge and test conditions.
The variation between the two blank controls was ± 2.3 %
Therfore, the test was considered valid.
Any other information on results incl. tables
Table 1: Respiration rates and inhibition of respiration of test and reference item concentrations
code |
Concentration [mg/L] |
Respiration Rate [mg O2/L x h] |
Inhibition [%] |
B1 |
control 0 |
76.0 |
- |
B2 |
control 0 |
79.6 |
- |
T1 |
125 |
76.7 |
1.5 |
T2 |
250 |
71.7 |
7.9 |
T3 |
500 |
71.7 |
7.9 |
T4 |
1000 |
64.0 |
17.8 |
T5 |
2000 |
59.6 |
23.5 |
R1 |
Reference 3 |
36.7 |
52.9 |
R2 |
Reference 10 |
21.5 |
72.4 |
R3 |
Reference 30 |
7.6 |
90.2 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- . The EC50 value was estimated to be > 2000 mg/L.
- Executive summary:
A Respiration Inhibition Test was performed with activated sludge according to OECD Guideline No. 209. The test system was non-adapted activated sludge of a municipal treatment plant. The test was performed under static conditions with the nominal concentrations of 125, 250, 500, 1000 and 2000 mg/L. The respiration rates of control, reference and test item concentrations were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates.
In order to check the activity of the test system and the test conditions a reference test was carried out with 3,5 -dichlorophenol as reference item and the reference toxicity was estimated to be 2.5 mg/L. The variation between the two blank controls was ± 2.3 %.
Validity criteria of the test guideline were fulfilled.
At the applied test concentrations of 2000 and 1000 mg/L respiration inhibitions of 23 and 18 % respectively, were observed. At the other nominal concentrations the test item showed no significant toxic effects (< 15 % respiration inhibition) to activated sludge; the respiration rates were in the same range as determined for the two blank controls.
Based on these data the EC20 of the test item was calculated to be 1450 mg/L. The EC50 and EC80 values were estimated to be > 2000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.