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Diss Factsheets
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EC number: 903-816-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to O.E.C.D. Testing Guideline 429 with GLP compliance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 3-chloropropene
- EC Number:
- 203-457-6
- EC Name:
- 3-chloropropene
- Cas Number:
- 107-05-1
- Molecular formula:
- C3H5Cl
- IUPAC Name:
- 3-chloroprop-1-ene
- Details on test material:
- As per the IUCLID5 Sections 1.1. - 1.4. for 3-chloropropene.
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- Animals were acquired from Harlan Laboratories UK Limited, Bicester, Oxon, UK, and were 8 - 12 wks old at study initiation. The weight range at study initiation was 15 to 23 gm. Animals were individually housed in suspended solid-floor polypropylene cages furnished with softwood woodflakes. Feed offered ad libitum was 2014 Teklad Global Rodent diet supplied by Harlan Teklad, Blackthorn, Bicester, Oxon, UK. Tap water was supplied ad libitum. The acclimation period > 5 days. The environmental conditions were: Temperature 19 to 25 °C, Humidity 30 to 70%, Air changes (per hr): 15 and the Photoperiod (hrs dark / hrs light) was 12/12.
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 25, 50 % (v/v) in acetone/olive oil and 100 % (undiluted)
- No. of animals per dose:
- 5
- Details on study design:
- The preliminary screening test suggested that 3-chloropropene would not produce excessive local irritation at the highest tested of 100%. Animals, were treated with 50 pl (25 pl per ear) of the undiluted test substance or the test substance as a solution in acetone/olive oil 4:1 at concentrations of 50% or 25% v/v. A further group of five animals was treated with acetone/olive oil 4:1 alone. A concurrent positive control test, using a
group of five animals, was also performed with the known sensitiser, a-Hexylcinnamaldehyde tech., 85%, at a concentration of 15% v/v in acetone/olive oil 4:1.
Five days after treatment the animals were injected via the tail vein with 250 uL phosphate buffered saline containing 3H-methyl thymidine at 20 uCi per mouse to label the lymph node DNA. After sacrifice the draining auricular lymph nodes were excised and processed to single cell suspensions. The radiolabeled DNA was precipated with 5% trichloroacetic. Samples were subjected to liquid scintillation counting by a Bechman LS6500 scintillation system. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- Data was processed to give group mean values for disintegrations per minute and standard deviations where appropriate. Individual and group mean disintegrations per minute values were assessed for dose response relationships by analysis of homogeneity of variance followed by one way analysis of variance (ANOVA). In the event of a significant result from the ANOVA, pairwise comparisons were performed between control and treated groups. For homogenous datasets Dunnett's Multiple Comparison test was used and for non-homogenous datasets Dunnett's T3 Multiple Comparison Method was used.
Results and discussion
- Positive control results:
- a-Hexylcinnamaldehyde tech., 85%, at a concentration of 15% v/v in acetone/olive oil 4:1 induced a positive stimulation index of 6.2.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: The Stimulation Index for the 25%, 50%, 100% test substance concentrations and positive control were: 0.78, 0.75, 1.97 and 6.2 respectively.
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: The mean DPM per animal for the Vehivle, 25%, 50% and 100% test substance were as follows: 1656 ±417; 1293 ±359; 1246 ±378 and 3255 ±139. respectively. Mean DPMs for the positive control were 10278 ±3234.
Any other information on results incl. tables
No adverse affects were observed concerning clinical signs or body weight effects.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- Treatment with the structural analog of AC Lighe Ends, 3-chloropropene, did not induce a state of dermal sensitization in the mouse local lymph node assay up to a high concentration level of 100%.
- Executive summary:
3 -Chloropropene, a structural analog for AC Light Ends was assessed for the ability to induce dermal sensitization in the mouse following an O.E.C.D. 429 Testing Guideline "Skin Sensitization Local Lymph Node Assay" with GLP compliance. Treatment with 3-chloropropene, did not induce a state of dermal sensitization in the mouse local lymph node assay up to a high concentration level of 100%. Therefore, it is anticipated that AC Light Ends will not be a dermal sensitizer in mouse LLNA system.
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