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EC number: 701-052-3 | CAS number: 146987-98-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well conducted and documented study according to guidelines, but limitation due to report language (german); not tested in E. coli WP2 uvra or S. typhimurium TA102
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Amides, C8-18, C18 unsatd, N-[3-(dimethylamino)propyl]
- IUPAC Name:
- Amides, C8-18, C18 unsatd, N-[3-(dimethylamino)propyl]
Constituent 1
Method
- Target gene:
- his operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- postmitrochondrial supernatant fluids fram the liver of male rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- 0.8. 4, 8, 20, 40, 100, 200, 500, 1000 and 5000 µg test item per plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Tween 80 in Wasser
- Justification for choice of solvent/vehicle: common solvent for suspensions
Controls
- Untreated negative controls:
- no
- Remarks:
- Suspension of tween 80 was used as negative control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Suspension of tween 80 in dest. water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Natriumazidm, 4-Nitro-o-phenylendiamine, 9-Am;noacridin, 2-Aminoanthracen
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in suspension
DURATION
- Exposure duration: 48h
SELECTION AGENT (mutation assays): histidine
NUMBER OF REPLICATIONS:3
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- - No growth inhibition of non reverted bacteria compared to negative controls
- Spontaneous mutation in the range of characteristic spontaneous mutation (table given in report).
- Positive controls shows a minimum mutation rate two times higher (TA 100, three times for all other strains) then control.
- A result is rated as positive if for more than one concentration the test substance increases the mutation rate two times compared t o the control for TA 100 and three times compared to the control for all other strains.
- Statistics:
- No specific statistical tests used.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- observed in the highest tested concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- observed in the highest tested concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without acroclor induced S9 mix metabolic activation
The examined test substance (Amides, C8-18, C18 unsatd, N-[3-(dimethylamino)propyl]) does not induce reverse mutation in the tested bacteria strains TA 1535, TA 100, TA 1537, TA 1538 and TA 98 with and without metabolic activation (aroclor induced rat liver S-9 mix). - Executive summary:
The sample of Amides, C8-18, C18 unsatd, N-[3-(dimethylamino)propyl] was tested for mutagenic activity in the bacterial tester strains Salmonella typhimurium TA 1535, TA 100, TA 1537, TA 1538 and TA 98. The test was conducted on agar plates in the absence or presence of postmitrochondrial supernatant fluids from the liver of male rats treated with Aroclor 1254 (S-9 mix). Suspensions of the test compound were freshly made up with Tween 80 in water just before use. The following concentrations were tested: 0.8, 4, 8, 20, 40, 100, 200, 500, 1000 and 5000 µg test item per plate.
Amides, C8-18, C18 unsatd, N-[3-(dimethylamino)propyl] did not induce reverse mutations in the presence and absence of S-9 mix in the tester strains TA 1535, TA 100, TA 1537, TA 1538 and TA 98. The test item did not show mutagenic activity in vitro.
The adopted OECD TG 471 (1997) requires the use of E. coli WP2 strains or Salmonella typhimurium TA 102 to detect certain oxidizing mutagens, cross-linking agents and hydrazines. However, the test substance is not a highly reactive agent and is therefore not expected to be a cross-linking agent, has no oxidizing properties and is no hydrazine. Thus, a test according to EU Method B.13/14 (Version Commission Directive 92/69/EEC without E. coli WP2 strains or Salmonella typhimurium TA 102 is considered as sufficient to evaluate the mutagenic activity of the test substance in this bacterial test system.
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