Dipotassium [[N,N'-ethylenebis[N-(carboxylatomethyl)glycinato]](4-)-N,N',O,O',ON,ON']zincate(2-)

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-01-04 to 2019-02-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants, Growth Test)

Version / remarks:

July 2006

Deviations:

yes

Remarks:

The room temperature and relative humidity were recorded continuously throughout the test with a data logger and a hygro-thermograph, respectively, due to technical reasons.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Determination of the test item
The concentration of the test item EDTA-ZnK2 was confirmed by analytical verification of the stock solution and control. The samples were analysed with a photometer method, implemented under non-GLP and documented finally in the GLP raw data.

Vehicle:

yes

Remarks:

Quartz sand

Details on preparation and application of test substrate:

The control and test item treatments were applied once at the beginning of the study. A stock solution of 65 g/L was prepared in demineralised water, diluted to various concentrations and incorporated into the soil. All concentrations of the test item were related to the dry weight of the soil. The test item solutions and additional demineralised water were added to the soil to adjust the humidity of the soil to a moisture of 45 % of the maximum water holding capacity (MWHC) were added to the soil. Subsequently, the soil was mixed carefully with a mixer (3 min) to ensure a homogeneous distribution. The treated soil was transferred to the test container and the seeds were sown.

Application Conditions of the Soil
Application date 2019-01-04 2019-01-22
Dry Weight (DW) of the soil before application [g/100 g soil WW] 95.3 96.1
Soil amount per batch [kg DW] 7.60* 5.5
[kg WW] 7.97 5.72
Maximum water holding capacity (MWHC) of the soil [g/100 g soil DW] 39.6 36.5
Added water amount per application to reach 45 % of MWHC [g/ 100 g DW] 12.89 12.37
Soil amount (with 45 % of MWHC) per replicate [kg] 0.70 0.70
* Due to large soil amounts per concentration, several batches of 7.60 kg DW were prepared per concentration and control and applied separately.

Species:

Avena sativa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

Common name: Oats
Plant family: Poaceae
Variety: KWS Contender
Source of seed: KWS Lochow GmbH, Ferdinand-von-Lochow-Straße 5, 29303 Bergen, Germany
Prior seed treatment / Sterilization: No
Seed storage: The seeds were stored dark at the test facility at room temperature (22 +/- 2 °C) and protected from moisture until use.

Species:

Brassica napus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

Common name: Rape
Plant family: Brassicaceae
Variety: Sherlock
Source of seed: KWS Saat SE, Grimsehlstraße 31, 37574 Einbeck, Germany
Prior seed treatment / Sterilization: No
Seed storage: The seeds were stored dark at the test facility at room temperature (22 +/- 2 °C) and protected from moisture until use.

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

Common name: Soybean
Plant family: Fabaceae
Variety: Obelix
Source of seed: Delley Samen und Pflanzen AG, Schloss Delley 40, route de Portalban, CH-1567 Delley, Switzerland
Prior seed treatment / Sterilization: No
Seed storage: The seeds were stored dark at the test facility at room temperature (22 +/- 2 °C) and protected from moisture until use.

Test type:

seedling emergence and seedling growth test

Study type:

laboratory study

Substrate type:

other: 2:1 Mixture of natural soil LUFA 2.2 (loamy sand (DIN classification)) and quartz sand

Limit test:

no

Total exposure duration:

21 d

Test temperature:

nominal: 22 +/- 10 °C; actual: 12.7 - 23.1 °C

pH:

5.36 ± 0.04 (soil)

Moisture:

relative humidity:
nominal: 70 +/- 30 %; 33.0 - 100 %

Details on test conditions:

TEST SYSTEM
- Testing facility: Noack Laboratorien GmbH
- Test container (type, material, size): Non-porous plastic containers (standard flower pots) with a diameter of ca. 12 cm were used. The pots were labelled with the study number and all necessary additional information to ensure unique identification.
- Amount of soil: 700 g per test container
- Method of seeding: Test containers were filled each with the same amount of treated soil (see application technique) and seeding holes were made with a seedling pistil. One seed was given into each hole and covered with soil. During cultivation the test containers were bottom watered and fertilized as necessary.
- No. of seeds per container: Oats, rape: 5; Soybean: 3
- No. of replicates per treatment group: 8
- No. of replicates per control: 8

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
A 2:1 mixture of natural soil LUFA 2.2 (batch number: Sp2.23618, loamy sand (DIN classification)) and quartz sand (12a) was used.
Grain size: <= 2 mm
Clay (< 0.002 mm): 6.0 %
Silt (0.002 - 0.063 mm): 9.1 %
Sand (0.063 - 2 mm): 84.9 %
Carbon content: 0.986 %
pH-value: 5.36 ± 0.04

NUTRIENT MEDIUM (if used)
HAKAPHOS® SOFT SPEZIAL 16+8+22(+3)
For watering a 0.05 ‰ solution was used
Origin: Grünes Landhaus, St. Godehard-Str. 23, 31139 Hildesheim, Germany

GROWTH CONDITIONS
- Photoperiod: 16/8
- Light source: Special-fluorescent tube Fluora and Daylight (Osram)
- Light intensity and quality: nominal: > 3500 lx; actual: 4444 ± 396
- Temperatures: nominal: 22 +/- 10 °C; 12.7 - 23.1 °C
- Relative humidity (%): nominal: 70 +/- 30 %; actual: 33.0 - 100 %
- Watering regime and schedules: Bottom watering of the test containers with fertilized tap water as needed.
- Interval of applications: once at experimental starting
- Method of application: spray application

ACCLIMATION PERIOD: None

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

- Phytotoxicity rating system (if used):
During the 21 day observation period (from day of application) the plants were observed on day 7, 14 and 21, for number of emerged seedlings, number of dead plants and visual phytotoxic effects.
The rating of the treated plants was done in relation to the untreated control plants. Observations included all variations, either inhibitory or stimulatory, between the treated replicates and the untreated controls. Such variations may be phytotoxic symptoms (e.g. chlorosis, necrosis, wilting), effects of growth and development rates. 
Observation Parameters
(1) Deformation of leaves (curling / twisting / sticking / …)
(2) Shoot deformations (curling / bending / …)
(3) Stunted growth
(4) Necrosis
(5) Chlorosis
(6) Lighter green
(7) Darker green
(8) Loss of colour
(9) Discoloration of leaves
(10) Wilting
(E) Dead plant (Number of dead plants)

Assessment of the Effects
- = no effects observed / normal growth
+/- = slight effects
+ = medium effects
++ = strong effects 


VEHICLE CONTROL PERFORMED: yes/no

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
The preliminary study was performed under the same laboratory conditions as specified for the definitive study with 4 replicates per concentration and control. Test duration was 21 days. At test end shoot fresh weight and emergence were determined.

Inhibition of Shoot Fresh Weight
Test concentration
[mg/kg DW] Inhibition [%]
Oats Rape Soybean
10 0 -20 34
100 -12 -11 -5
1000 12 12 44

Table 41: Inhibition of Rate of Emergence
Test concentration
[mg/kg DW] Inhibition [%]
Oats Rape Soybean
10 0 0 0
100 0 5 0
1000 0 0 23
Negative values = promoted growth / promoted emergence
DW = soil dry weight

Nominal and measured concentrations:

Nominal: 1000 - 500 - 250 - 125 - 62.5 mg/kg DW

Reference substance (positive control):

no

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoor fresh weight

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

447 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Remarks on result:

other:

Remarks:

CI (95%) = 258 - 763 mg/kg soil DW

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

125 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

223 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Remarks on result:

other:

Remarks:

CI (95 %) = 144 - 322 mg/kg sol DW

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

491 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Remarks on result:

other:

Remarks:

CI (95%) = 324 - > 1000 mg/kg soil DW

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

674 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Remarks on result:

other:

Remarks:

CI (95%) = 572 - 776 mg/kg soil DW

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot height

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

125 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Remarks:

shoot fresh weight

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

805 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Remarks on result:

other:

Remarks:

CI (95%) = 584 - 833 mg/kg soil DW

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

shoot fresh weight

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC25

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Details on results:

The percentage of inhibition of shoot height, shoot fresh weight and rate of emergence is given below. Test item-related statistically significant inhibitions are given in bold. The tabulated results represent rounded mean values calculated on the exact raw data.

Oats: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW] Shoot
height
[cm] Inhibition

[%] Shoot fresh weight
[mg] Inhibition

[%] Rate of emergence1)
[%] Inhibition

[%]
Control 42.1 - 1495 - 93 -
62.5 41.4 2 1250 16 100 -8
125 42.4 -1 1327 11 95 -2
250 41.2 2 1208 19 95 -2
500 41.6 1 1256 16 98 -5
1000 42.4 -1 1386 7 95 -2

Rape: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW] Shoot
height1)
[cm] Inhibition

[%] Shoot fresh weight
[mg] Inhibition

[%] Rate of emergence1)
[%] Inhibition

[%]
Control 17.8 - 1739 - 85 -
62.5 16.3 8 1749 -1 90 -6
125 16.3 8 1566 10 85 0
250 15.0 16 1250 28 88 -4
500 12.4 30 853 51 93 -9
1000 11.8 34 660 62 98 -15
 
Soybean: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW] Shoot
height2)
[cm] Inhibition

[%] Shoot fresh weight
[mg] Inhibition

[%] Rate of emergence3)
[%] Inhibition

[%]
Control 22.1 - 2815 - 96 -
62.5 22.5 -2 2939 -4 96 0
125 22.0 0 2864 -2 92 4
250 20.2 9 2731 3 92 4
500 19.3 13 2613 7 100 -4
1000 11.4 48 1837 35 79 18
Negative values = Promoted growth
bold = Statistically significant inhibition
1) Normality test failed
2) Equal variance test failed
3) Normality and equal variance test failed

Results with reference substance (positive control):

None

Reported statistics and error estimates:

Percentage of inhibition
The percentage of inhibition of biomass growth (shoot height, shoot fresh weight) and rate of emergence were calculated in relation to the control. All dead plants were excluded from the calculation of shoot height and fresh weight (above-ground biomass).

NOEC / LOEC of biomass growth / emergence
One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to control replicates. When running a One Way Analysis of Variance, a Normality test (Shapiro-Wilk) and an Equal Variance test (Brown-Forsythe) were done first. P-values for both, Normality and Equal Variance test, are 0.05. The alpha-value for ANOVA (acceptable probability of incorrectly concluding that there is a difference) is alpha = 0.05.

Failure of the normality test and / or the equal variance test can be caused by extremely homogeneous emergence and growth patterns as opposed to higher variances in other treatments. Due to the high and even number of replicates in the control and treatment groups, the failure had no influence on the robustness of the calculations.

EC-values and confidence intervals of biomass growth
EC25- and EC50- values were calculated by sigmoidal dose-response regression using GraphPadPrism. Four parameter were used for dose-response fitting. Calculation of the confidence intervals for EC-values was carried out using standard procedures provided by GraphPadPrism.

Validity criteria fulfilled:

yes

Conclusions:

Potential toxic effects of the test item were assessed on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and at test end (day 21) by shoot height and shoot fresh weight determination.
The results of the control for all plant species met the required validity criteria. Therefore, the test is considered to be valid.

Shoot Height: NOEC, LOEC, EC25- and EC50-Values with Confidence Range (p)
Species NOEC
[mg/kg DW] LOEC
[mg/kg DW] EC25
[mg/kg DW] p = 95%
[mg/kg DW] EC50
[mg/kg DW] p = 95%
[mg/kg DW]
Oats ≥ 1000 > 1000 > 1000 ̶ > 1000 ̶
Rape 250 500 447 258 - 763 > 1000 ̶
Soybean 250 500 674 572 - 776 > 1000 –

Shoot Fresh Weight: NOEC, LOEC, EC25- and EC50-Values with Confidence Range (p)
Species NOEC
[mg/kg DW] LOEC
[mg/kg DW] EC25
[mg/kg DW] p = 95%
[mg/kg DW] EC50
[mg/kg DW] p = 95%
[mg/kg DW]
Oats ≥ 1000 > 1000 > 1000 ̶ > 1000 ̶
Rape 125 250 223 144 - 322 491 324 - >1000
Soybean 500 1000 805 584 - 833 > 1000 –


Number of Emerged Seedlings: NOEC, LOEC, EC25- and EC50-Values with Confidence Range (p)
Species NOEC
[mg/kg DW] LOEC
[mg/kg DW] EC25
[mg/kg DW] p = 95%
[mg/kg DW] EC50
[mg/kg DW] p = 95%
[mg/kg DW]
Oats ≥ 1000 > 1000 > 1000 ̶ > 1000 ̶
Rape ≥ 1000 > 1000 > 1000 ̶ > 1000 ̶
Soybean ≥ 1000 > 1000 > 1000 ̶ > 1000 ̶
-) Not determinable 

Executive summary:

Summary

The phytotoxicity of the test itemEDTA-ZnK2(batch number:FC-C11530) to three terrestrial plant species was determined according to OECD 208 (July 2006) atthe test facilityin a seedling emergence and seedling growth tests over a period of 21 days, from2019-01-04to2019-02-12.

Test Design

The test was conducted with the test concentrations of1000 - 500 - 250 - 125 - 62.5 mg/kg DW (factor 2).The test item was incorporated into the soil in which the seeds were sown. The test containers were bottom watered with nutrient solution as needed.

 

Results

The stock solutions were sampled prior to application and subsequently analytically verified. The measured concentrations ofEDTA-ZnK2were 95% and 98% indicating the correct preparation of the stock solution.

 

Potential toxic effects of the test item were assessedon day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) andat test end by shoot height and shoot fresh weight determination.

The results of the control for all plant species met the required validity criteria. Therefore, the test is considered to be valid.

 

Shoot Height: NOEC, LOEC, EC₂₅- and EC₅₀-Values with Confidence Range (p)

Species

NOEC [mg/kg DW]

LOEC [mg/kg DW]

EC25 [mg/kg DW]

p = 95% [mg/kg DW]

EC50 [mg/kg DW]

p = 95% [mg/kg DW]

Oats

≥ 1000

> 1000

> 1000

̶

> 1000

̶

Rape

250

500

447

258 - 763

> 1000

̶

Soybean

250

500

674

572 - 776

> 1000

–

 

Shoot Fresh Weight: NOEC, LOEC, EC₂₅- and EC₅₀-Values with Confidence Range (p)

Species	NOEC [mg/kg DW]	LOEC [mg/kg DW]	EC25 [mg/kg DW]	p = 95% [mg/kg DW]	EC50 [mg/kg DW]	p = 95% [mg/kg DW]
Oats	≥ 1000	> 1000	> 1000	̶	> 1000	̶
Rape	125	250	223	144 - 322	491	324 - >1000
Soybean	500	1000	805	584 - 833	> 1000	–

Number of Emerged Seedlings: NOEC, LOEC, EC₂₅- and EC₅₀-Values with Confidence Range (p)

Species	NOEC [mg/kg DW]	LOEC [mg/kg DW]	EC25 [mg/kg DW]	p = 95% [mg/kg DW]	EC50 [mg/kg DW]	p = 95% [mg/kg DW]
Oats	≥ 1000	> 1000	> 1000	̶	> 1000	̶
Rape	≥ 1000	> 1000	> 1000	̶	> 1000	̶
Soybean	≥ 1000	> 1000	> 1000	̶	> 1000	̶

-) Not determinable

Description of key information

One toxicity to terrestrial plant tests is performed with EDTA-ZnK2 according to OECD 208 using three different plant species. the lowest NOEC of 125 mg test item/kg dw was observed for shoot wet weight of Brassica napus (rape). The lowest EC50 of 491 mg test item/kg dw was also observed shoot wet weight of Brassica napus (rape). The test item contained 90.4% active ingredient. The effect data based on active are then: NOEC of 113 mg a.i./kg dw was observed for shoot wet weight of Brassica napus (rape). The lowest EC50 of 444 mg a.i./kg dw also for Brassica napus (rape)

Key value for chemical safety assessment

Short-term EC50 or LC50 for terrestrial plants:

444 mg/kg soil dw

Long-term EC10, LC10 or NOEC for terrestrial plants:

113 mg/kg soil dw

Additional information

Summary

The phytotoxicity of the test item EDTA-ZnK2 (batch number:FC-C 11530) to three terrestrial plant species was determined according to OECD 208 (July 2006) at the test facility in a seedling emergence and seedling growth tests over a period of 21 days.

Test Design

The test was conducted with the test item concentrations of 1000 - 500 - 250 - 125 - 62.5 mg/kg DW (factor 2).The test item was incorporated into the soil in which the seeds were sown. The test containers were bottom watered with nutrient solution as needed.

 

Results

The stock solutions were sampled prior to application and subsequently analytically verified. The measured concentrations of EDTA-ZnK2 were 95% and 98% indicating the correct preparation of the stock solution.

 

Potential toxic effects of the test item were assessed on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and at test end by shoot height and shoot fresh weight determination.

The results of the control for all plant species met the required validity criteria. Therefore, the test is considered to be valid.

 

Shoot Height: NOEC, LOEC, EC₂₅- and EC₅₀-Values with Confidence Range (p)

Species

NOEC [mg/kg DW]

LOEC [mg/kg DW]

EC25 [mg/kg DW]

p = 95% [mg/kg DW]

EC50 [mg/kg DW]

p = 95% [mg/kg DW]

Oats

≥ 1000

> 1000

> 1000

̶

> 1000

̶

Rape

250

500

447

258 - 763

> 1000

̶

Soybean

250

500

674

572 - 776

> 1000

–

 

Shoot Fresh Weight: NOEC, LOEC, EC₂₅- and EC₅₀-Values with Confidence Range (p)

Species	NOEC [mg/kg DW]	LOEC [mg/kg DW]	EC25 [mg/kg DW]	p = 95% [mg/kg DW]	EC50 [mg/kg DW]	p = 95% [mg/kg DW]
Oats	≥ 1000	> 1000	> 1000	̶	> 1000	̶
Rape	125	250	223	144 - 322	491	324 - >1000
Soybean	500	1000	805	584 - 833	> 1000	–

EC25 [mg/kg DW]

p = 95% [mg/kg DW]

EC50 [mg/kg DW]

p = 95% [mg/kg DW]

Oats

≥ 1000

> 1000

> 1000

̶

> 1000

̶

Rape

250

500

447

258 - 763

> 1000

̶

Soybean

250

500

674

572 - 776

> 1000

–

 

Shoot Fresh Weight: NOEC, LOEC, EC₂₅- and EC₅₀-Values with Confidence Range (p)

Species	NOEC [mg/kg DW]	LOEC [mg/kg DW]	EC25 [mg/kg DW]	p = 95% [mg/kg DW]	EC50 [mg/kg DW]	p = 95% [mg/kg DW]
Oats	≥ 1000	> 1000	> 1000	̶	> 1000	̶
Rape	125	250	223	144 - 322	491	324 - >1000
Soybean	500	1000	805	584 - 833	> 1000	–