4,11-diamino-2-[3-(2-methoxyethoxy)propyl]-1H-naphth[2,3-f]isoindole-1,3,5,10(2H)-tetrone

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Principles of method if other than guideline:

None

GLP compliance:

yes

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

other: Mixture of polyvalent bacteria collected on May 16, 1994 in the aeration tank of a domestic sewage treatment plant, ARA Basel Communal.

Details on inoculum:

- Bacteria concentration: 30 mg/l suspended solids

Duration of test (contact time):

22 d

Details on study design:

- Water / Nutrient: According to the specification of the test method
- Temperature: 22 ± 2 °C

Reference substance:

other: Natriumbenzoat [Merck ArtNr. 6290]

Preliminary study:

None

Test performance:

None

Key result

Parameter:

% degradation (test mat. analysis)

Value:

0

Sampling time:

21 d

Details on results:

None

Results with reference substance:

Biodegradation after 21 days of the reference substance is 86.8 %

None

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

FAT 36152/G is not inherently biodegradable.

Executive summary:

The ready biodegradability of FAT 36152/G was determined in a 21 days Manometric Respirometry-Test according to the EEC-Directive No 92/69, Annex L 383A and to the OECD Guideline for Testing of Chemicals, No. 301F, Paris 1981. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in a concentration of 100 mg/I. The reference substance (Natriumbenzoat) was also tested in a concentration of 100 mg/I. Biodegradation after 21 days of the test substance of FAT 36152/G was 0 % , while it was found to be 86.8 % for reference substance. Hence, FAT 36152/G is considered as not inherently biodegradable.

Description of key information

The target chemical is considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

In a GLP-compliant study performed to determine the chemical oxygen demand of FAT 36152/G according to DEV DIN 38'409 - H 41-1 (1980) corresponding to 92/96/EE C C.6. A predetermined amount of the test substance in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate was determined by titration with standardized ferrous ammonium sulphate. The COD of FAT 36152/F was determined to be 1567 mg O2/g test material. Another study was performed to determine the ready biodegradation of FAT 36152/F in a 21 days Manometric Respirometry Test according to the EEC-Directive No 92/69, Annex L 383A and to the OECD Guideline for Testing of Chemicals, No. 301F, Paris 1981. The test substance was tested in a concentration of 100 mg/I. Biodegradation after 21 days of the test substance of FAT 36152/G is: 0 %. Therefore, FAT 36152/G is not readily biodegradable. In studies performed according to the EEC Directive, COD & BOD5 values were determined to be 417 mg O2/g test mat and 4 mg O2/g test mat respectively. Further, the BOD5/COD quotient calculated using data from the studies is equivalent to 0, indicating the test substance as not rapidly biodegradable. 50.65 % biodegradation (Adsorption 3 h:16:35 %) was observed in a 28-day biodegradation test performed as per OECD guideline 302B. OECD Guideline No 302B does not give any definitive pass levels for test items, however the “Summary of Considerations in the Report from the OECD Expert Group on Degradation and Accumulation” suggests that a figure of more than 20 % biodegradation may be regarded as evidence for inherent, primary biodegradability. A figure of more than 70 % mineralisation may be regarded as evidence for ultimate biodegradation. FAT 36152/D is found to be inherently biodegradable under the conditions of this test. Considerating the data from the key and supporting studies and the BOD5/COD quotient which is not >0.5 the target chemical is considered to be not readily biodegradable.