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Diss Factsheets
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EC number: 200-386-2 | CAS number: 58-56-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5-31 October 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was conducted according to OECD Guidelines and in compliance with GLP principles. Although no details are included in the report on analytical validation, the method is deemed sufficiently validated as documented in the report of the Daphnia magna acute toxicity test (Ciba 928379, reliable with restrictions based on lack of detailed method validation results).
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Composite samples (about 150 mL in duplicate) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 96 hours exposure and kept at - 18 ºC to - 22 ºC until analysis.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: calculated amounts of the stock solution (7.5 g test substance with 1000 mL water) to produce the desired test concentrations were homogeneously distributed into the water. The solutions were homogenuous at all test times and test concentrations. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: P. Hohler/ CH-4314 Zeiningen
- Length at study initiation (mean): 51 mm (47-54 mm) based on 10 fishes
- Weight at study initiation (mean): 1.25 (0.94-1.59 g) based on 10 fishes
- Method of breeding: not reported
- Feeding during test: none
ACCLIMATION
- Acclimation period: 48 days
- Adaptation: 24 hours/no food 24 hours prior to exposure - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 168 mg CaCO3/L
- Test temperature:
- 15 ºC
- pH:
- At the start: 7.3 - 7.6
Day 1 - Day 4: 8.0 - 8.2 - Dissolved oxygen:
- 84 - 107% of the air saturation value (no raw data reported).
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal: 10, 18, 32, 58, and 100 mg/L
Measured: see table below. The measured concentrations were within ± 20% of nominal concentrations at the start and end of the test. The effect concentrations were reported as nominal concentrations, which is acceptable. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 20 L filled with 15 L
- size: 36/22/25 cm
- Aeration: gentle aeration during exposure
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.42 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light
- Light intensity: Fluorescent light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortalities after 24, 48, 72 and 96 hours; daily examination for symptoms of swimming behaviour, loss of equilibrium, respiratory function, exoophtalmus and pigmentation
TEST CONCENTRATIONS
- Spacing factor for test concentrations:1.8
- Range finding study: conducted but not reported - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortalities or other symptoms were observed in any of the treatments.
- Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h-LC50 of the test substance was > 100 mg/L (based on analytically confirmed nominal concentrations).
- Executive summary:
In a 96h acute toxicity study conducted according to OECD 203, rainbow trout (Oncorhynchus mykiss) were exposed to Pyridoxin-HCl under static conditions at the following nominal concentrations: blank-control, 10, 18, 32, 56, 100 mg/L. Test substance induced no visible or lethal effects in the fish at any of the concentrations tested. The 96 h LC50 was >100 mg/L based on analytically confirmed nominal concentrations. Although no details were included in the report on analytical validation, the method is deemed sufficiently validated as documented in the report of the Daphnia magna acute toxicity test (Ciba 928379, reliable with restrictions based on lack of detailed method validation results). The short-term toxicity test to rainbow trout is therefore considered to be also reliable with restrictions.
Reference
Description of key information
In a 96h acute toxicity study conducted according to OECD 203, rainbow trout (Oncorhynchus mykiss) were exposed toPyridoxin-HClunder static conditions at the following nominal concentrations: blank-control, 10, 18, 32, 56, 100 mg/L. Test substance induced no visible or lethal effects in the fish atany of the concentrations tested.The 96 h LC50 was >100 mg/L based on analytically confirmed nominal concentrations.Although no details were included in the report on analytical validation, the method is deemed sufficiently validated as documented in the report of the Daphnia magna acute toxicity test (Ciba 928379, reliable with restrictions based on lack of detailed method validation results). The short-term toxicity test to rainbow trout is therefore considered to be also reliable with restrictions.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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