REAKTIV-ORANGE DYPR 934

Solubility in organic solvents / fat solubility

Administrative data

Endpoint:

solubility in organic solvents / fat solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-12-08 to 1999-12-16

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 116 (Fat Solubility of Solid and Liquid Substances)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Reaktiv-Orange DYPR934

Results and discussion

Solubility in organic solvents / fat solubility

Medium:

other: Fat: standard fat HB 307, charge 31 (Natec Hamburg)

Solubility:

< 0.003 other: mg/L in 100 g fat

Temp.:

37 °C

Details on results:

- For raw data see Table [1].

Any other information on results incl. tables

 Table 1:

Details on Experiments

Experiment No.	Pre-saturation		Stirring time at 37 °C (h)	Mass ratio Dissolved test substance/fat (mg/100g)
	Stirring time (h)	Temperature (°C)
1	3.0	30	3	<0.004
2	3.0	30	3	<0.004
3	3.0	30	27	<0.004
4	3.0	30	27	<0.004
5	3.0	30	3	<0.004
6	3.0	30	3	<0.004
7	3.0	30	27	<0.004
8	3.0	30	27	<0.004

Thus the ratio test substance per 100 g fat was calculated from the concentration of the lowest calibration standard (detection limit).

For evaluation of the result one has to take into account that the spectrometric measurements only include organic components showing absorption at the quantification wavelength.

A correlation with the purity of 56 % (w/w) yields the fat solubility of the substance.

Analytical method

Instrument	Spectrometer Beckman DU 70 (quartz cuvettes, path length 5 cm)
Measurement	Registration of the absorption spectra from 200 nm to 800 nm, evaluation at absorption maximum at circa 487 nm using the Net A2 evaluation (base 600 nm)
calibration	Recording absorption spectra of solutions of the test substance in distilled water.Concentrations range: 0.05-0.97 mg/L
Test Temperature	37 °C
Fat	Standard fat HB 307, charge 31 (Natec, Hamburg)

 

Applicant's summary and conclusion

Conclusions:

The fat solubility of the test substance is:
s (Reaktiv Orange DYPR 934) < 0.003 mg in 100 g fat at 37 °C.

Executive summary:

The fat solubility of the test substance is:

s (Reaktiv Orange DYPR 934) < 0.003 mg in 100 g fat at 37 °C.