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Diss Factsheets
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EC number: 221-877-8 | CAS number: 3266-23-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- sub-chronic toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented study report which meets basic scientific principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 961
- Report date:
- 1961
Materials and methods
- Principles of method if other than guideline:
- Four groups of 8 Guinea pigs per sex were exposed to 400 and 800 ppm (1.18 mg/l air and 2.38 mg/l air) for 7 hours daily on 135 to 141 days over a period of 198 days.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- 2,3-epoxybutane
- EC Number:
- 221-877-8
- EC Name:
- 2,3-epoxybutane
- Cas Number:
- 3266-23-7
- Molecular formula:
- C4H8O
- IUPAC Name:
- 2,3-dimethyloxirane
- Details on test material:
- - Name of test material (as cited in study report): Butylene oxide (varable components)
- Molecular formula (if other than submission substance): C4H8O
- Physical state: liquid, clear
- Composition of test material, percentage of components: 1-butylene oxide (70-90 +/- 3%), 2-butylene oxide (5-15 +/-3%), isobutylene oxide (2-10 +/- 3%), trace contaminents
Constituent 1
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Housing: in wire mesh bottom cages
- Diet (e.g. ad libitum): commercial chow plus cabbage; ad libitum except during exposure
- Water (e.g. ad libitum): water; ad libitum except during exposure
Administration / exposure
- Route of administration:
- inhalation
- Type of inhalation exposure:
- not specified
- Vehicle:
- other: no data
- Remarks on MMAD:
- MMAD / GSD: no data
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: sheat-metal chamber (ca. 1760 l)
- System of generating particulates/aerosols: required amounts of substance was metered into a vapourizing flask where it was mixed with air and then evenly distributed throughout the exposure chamber by means of a large circulating fan - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- by method of Hollingsworth and Waling (1955)
- Duration of treatment / exposure:
- 7 h/d, 135 - 141 days
- Frequency of treatment:
- daily
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
400, 800 ppm
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
1.18, 2.34 mg/l air
Basis:
nominal conc.
- No. of animals per sex per dose:
- 8
- Control animals:
- yes, concurrent no treatment
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes
- Time schedule: frequently
BODY WEIGHT: Yes
- Time schedule for examinations: every 5th week - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: Yes: lung, heart, kidneys, live, spleen, testes
Results and discussion
Results of examinations
- Details on results:
- CLINICAL SIGNS AND MORTALITY
- 800 ppm: nothing abnormal noted
- 400 ppm: nothing abnormal noted
BODY WEIGHT AND WEIGHT GAIN
- 800 ppm: depression of growth of males, significant depression of average final body weight of the males
- 400 ppm: after 12-15 weeks reduced growth of males, but not significant
HAEMATOLOGY
- 800 ppm: nothing abnormal noted
- 400 ppm: nothing abnormal noted
CLINICAL CHEMISTRY
- 800 ppm: nothing abnormal noted
- 400 ppm: nothing abnormal noted
ORGAN WEIGHTS
- 800 ppm: increase in lung weights of males and females, significant decrease in the average liver weight of females
- 400 ppm: nothing abnormal noted
HISTOPATHOLOGY:
- 800 ppm: nothing abnormal noted
- 400 ppm: nothing abnormal noted
Effect levels
open allclose all
- Dose descriptor:
- NOAEC
- Effect level:
- 400 ppm
- Sex:
- male/female
- Basis for effect level:
- other: decreased lung weights, body weights
- Dose descriptor:
- NOAEC
- Effect level:
- 1.18 mg/L air
- Sex:
- male/female
- Basis for effect level:
- other: decreased lung weights, body weights
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
Average final body weight and organ weight after 400 and 800 ppm exposure:
Sex | Exposure | No. Animals | Body weight (g) | Organ weight in g/100 g body weight | ||||||
Lung | Heart | Liver | Kidney | Spleen | Testes | |||||
400 ppm | Male | control | 7 | 966 | 0.73 | 0.27 | 2.84 | 0.6 | 0.12 | 0.42 |
exposed | 7 | 862* | 0.7 | 0.27 | 3.17* | 0.6 | 0.13 | 0.48 | ||
Female | control | 7 | 874 | 0.75 | 0.27 | 3.6 | 0.61 | 0.16 | - | |
exposed | 8 | 813* | 0.82 | 0.27 | 3.28 | 0.61 | 0.16 | - | ||
800 ppm | Male | control | 8 | 934 | 0.65 | 0.28 | 3.08 | 0.5 | 0.12 | 0.43 |
exposed | 7 | 827* | 0.83** | 0.29 | 2.88 | 0.61 | 0.13 | 0.49 | ||
Female | control | 8 | 815 | 0.74 | 0.26 | 3.37 | 0.6 | 0.17 | - | |
exposed | 7 | 782 | 1.04** | 0.27 | 2.94* | 0.59 | 0.15 | - |
*p<0.05; **p<0.01;***p<0.001
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.