2-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]benzoxazole-5-sulphonamide

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from J-CHECK authoritative database

Data source

Reference

Reference Type:

other: authoritative database

Title:

Bioaccumulation: aquatic/sediment

Author:

National Institute of Technology and Evaluation.

Year:

2017

Bibliographic source:

Japan chemicals collaborative knowledge database (J-check), 2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"

Principles of method if other than guideline:

Bioaccumulation study was conducted on test organism Cyprinus carpio for 28 days for evaluating the bioconcentration factor (BCF value) of 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate. The study was performed according to OECD Guideline 305 (Bioconcentration: Flow-through Fish Test) and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen 3,6-bis(diethylamino)-9-(2,4-disulphonatophenyl)xanthylium, sodium salt

EC Number:

222-529-8

EC Name:

Hydrogen 3,6-bis(diethylamino)-9-(2,4-disulphonatophenyl)xanthylium, sodium salt

Cas Number:

3520-42-1

Molecular formula:

C27H30N2O7S2.Na

IUPAC Name:

2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate

Test material form:

solid

Specific details on test material used for the study:

- Name of test material (IUPAC name): 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate
- Common name: C. I. Acid Red 52
- Molecular formula: C27H30N2O7S2.Na
- Molecular weight: 580.6551 g/mol
- Smiles notation: CCN(CC)c1ccc2c(c1)[o+]c3cc(ccc3c2c4ccc(cc4S(=O)(=O)[O-])S(=O)(=O)[O-])N(CC)CC.[Na+]
- InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
- Substance type: Organic
- Physical state: solid
- Purity: 84.4%
- Water solubility: ≥ 100 g/l (20°C)

Radiolabelling:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Cyprinus carpio

Study design

Route of exposure:

aqueous

Test type:

static

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Test conditions

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations : LC50(96h) > 200 mg/L, Rice fish (Oryzias latipes)

Nominal and measured concentrations:

Nominal concentrations:
1st concentration area: 1.69 mg/l (1690 µg/l)
2nd concentration area: 0.169 mg/l (169 µg/l)

Reference substance (positive control):

not specified

Results and discussion

Lipid content

Lipid content 1

Lipid content:

5.09 %

Time point:

start of exposure

Lipid content 2

Lipid content:

6 %

Time point:

end of exposure

Bioaccumulation factor

Bioaccumulation factor 1

Conc. / dose:

1.69 mg/L

Type:

BCF

Value:

<= 0.57 L/kg

Basis:

total lipid content

Time of plateau:

28 d

Remarks on result:

other: Other details not known

Bioaccumulation factor 2

Conc. / dose:

0.169 mg/L

Type:

BCF

Value:

<= 5.3 L/kg

Basis:

total lipid content

Time of plateau:

28 d

Remarks on result:

other: Other details not known

Any other information on results incl. tables

Bioaccumulation test results:

 

		10 d	14 d	19 d	24 d	28 d
1st concentration area	Test substance concentration (microg/L)	1610	1670	1620	1640	1650
	Bioconcentration factor (L/kg)	<= 0.57	<= 0.57	<= 0.57	<= 0.57	<= 0.57
		<= 0.57	<= 0.57	<= 0.57	<= 0.57	<= 0.57
2nd concentration area	Test substance concentration (microg/L)	181	172	176	177	170
	Bioconcentration factor (L/kg)	<= 5.3	<= 5.3	<= 5.3	<= 5.3	<= 5.3
		<= 5.3	<= 5.3	<= 5.3	<= 5.3	<= 5.3

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor (BCF value) of substance 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate on Cyprinus carpio was determined to be ≤ 0.57 L/Kg at a conc. of 1.69 mg/l and ≤ 5.3 L/Kg at a conc. of 0.169 mg/l, respectively.

Executive summary:

Bioaccumulation study was conducted on test organism Cyprinus carpiofor 28 days for evaluating the bioconcentration factor (BCF value) of 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (CAS no. 3520-42-1).The study was performed according toOECD Guideline 305 (Bioconcentration: Flow-through Fish Test) and other guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were1.69 mg/land 0.169 mg/l, respectively. Analytical method involve the recovery ratio:Fish : 89.6 % - Limit of quantitation : Test water : 1st concentration area : 83 microg/L 2nd concentration area : 8.3 microg/L Fish: 940 ng/g.Range finding study involve the LC50(96h) > 200 mg/LonRice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be 5.09% and 6% at the start and end of the exposure. The bioconcentration factor (BCF value) of substance 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate on Cyprinus carpio was determined to be≤ 0.57 L/Kg at a conc. of 1.69 mg/l and ≤ 5.3 L/Kg at a conc. of 0.169 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate is not expected to bioaccumulate in the food chain.