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EC number: 280-068-8 | CAS number: 82933-90-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: BC3384Y14 (India) - Analytical monitoring:
- yes
- Details on sampling:
- For range finding experiment the concentrations selected were 0.1, 1, 10, 25, 50 and 100 mg/L which is equivalent to, 0.06, 0.6, 6, 15, 30 and 60 mg/600ml, respectively. 100 mg of the test item was dissolved and made up to 100 ml with test medium (20X-AAP) in standard flask to make a stock concentration of 1 mg/ml. From the stock following concentrations were prepared. All the required test concentrations were prepared for 600 ml. For, 0.06, 0.6, 6 and 60 mg/600ml, 60 µl, 600 µl, 6 ml and 60 ml was taken from stock and made up to 600 ml with 20X-AAP medium respectively. For 15mg/600ml 15 mg of test item was directly added to the 600ml 20X-AAP medium. For Control, 20X-AAP medium was used. For Limit concentration ,100 mg/L which is equivalent to 130mg/1300ml. 130.04 mg of the test item was dissolved and made up to 1300 ml of 20X-AAP medium. From this 1200 ml of stock was directly used. For Control, 20X-AAP medium was used.
- Vehicle:
- yes
- Details on test solutions:
- For Limit concentration ,100 mg/L which is equivalent to 130mg/1300ml. 130.04 mg of the test item was dissolved and made up to 1300 ml of 20X-AAP medium. From this 1200 ml of stock was directly used. For Control, 20X-AAP medium was used.
- Test organisms (species):
- Lemna gibba
- Details on test organisms:
- TEST ORGANISM
- Strain: Lemna gibba (Duckweed)
- Source (laboratory, culture collection): In-house culture, RCC Laboratories India Private Limited (Originally procured by Department of Plant Biology & Plant Biotechnology Madras Christian College, Chennai) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 7 d
- Post exposure observation period:
- Frond number in each replicate was counted on days ‘0’, ‘3’, ‘5’ and ‘7’. A representative sample (approximately 12 fronds) of pre-culture was checked for its average dry weight on day ‘0’ of exposure and dry weight of plant material from each test and control vessel on day ‘7’ of exposure. Observation was performed on day ‘0’, ‘3’, ‘5’ and ‘7’ for the abnormal appearance or any developmental changes due to the effect of the test item (if any).
- Hardness:
- None
- Test temperature:
- Temperature in test vessel (°C) Start of the test
Control: 23.5
Treatment: 23.5
End of the test
Control: 24.8-25.1
Treatment: 25.1-25.3 - pH:
- Medium pH range Start of the test
Control: 7.54
Treatment: 7.48
End of the test
Control: 8.69-8.72
Treatment: 8.72-8.75 - Dissolved oxygen:
- None
- Salinity:
- None
- Nominal and measured concentrations:
- None
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 ml Glass Beaker
- No. of organisms per vessel: 12 fronds (3 plants)
- No. of vessels per concentration (replicates): Main Experiment - 3 replicates for each concentration
- No. of vessels per control (replicates): Control - 6 Replicates.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: Start of the test
- Photoperiod: Continuous Light
- Light intensity and quality: 8460-8650 Lux
TEST CONCENTRATIONS
-For range finding experiment the concentrations selected were 0.1, 1, 10, 25, 50 and 100 mg/L.
For Limit concentration ,100 mg/L which is equivalent to 130 mg/1300 ml. 130.04 mg of the test item was dissolved and made up to 1300 ml of 20X-AAP medium. From this 1200 ml of stock was directly used. For control, 20X-AAP medium was used. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry Weight; Growth
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Dry Weight; Yield
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Results with reference substance (positive control):
- ErC50 of 3,5-Dichlorophenol based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 4.30 mg/L with a lower confidence limit of 4.15 mg/L and the upper confidence limit of 4.46 mg/L.
EyC50 of 3,5-Dichlorophenol based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was calculated as 2.38 mg/L with a lower confidence limit of 2.29 mg/L and the upper confidence limit of 2.48 mg/L.
ErC50 of 3,5-Dichlorophenol based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was calculated as 3.02 mg/L with a lower confidence limit of 2.89 mg/L and the upper confidence limit of 3.16 mg/L.
EyC50 of 3,5-Dichlorophenol based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was calculated as 1.53 mg/L with a lower confidence limit of 1.46 mg/L and the upper confidence limit of 1.59 mg/L. - Reported statistics and error estimates:
- Mean coefficient of variation of average specific growth rate in replicate control and treated cultures were calculated. No analysis was done as the study is concluded with the limit test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- ErC50 of FAT 20043/E based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was founed to be >100 mg/L.
EyC50 of FAT 20043/E based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was founed to be >100 mg/L
ErC50 of FAT 20043/E based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was founed to be >100 mg/L
EyC50 of FAT 20043/E based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was founed to be >100 mg/L. - Executive summary:
In a GLP compliant OECD guideline 221 followed study, Lemna gibba (Duckweed) were exposed to the test item (FAT 20043/E) at test concentration 100 mg/L. Initially on day ‘0’, 12 fronds were inoculated in beaker containing 200 ml of test medium (20X-AAP medium). Three replicates for each test concentration and six replicates for control were maintained. The test item was formulated in 20X-AAP medium. Prior to exposure,Lemna gibba was pre-cultured in the 20X-AAP medium for period of ‘8’ days and the pre-culture was found healthy. After exposure,Lemna gibba was observed for frond count on days ‘3’, ‘5’ and ‘7’. Dry weight of the fronds were recorded at start (pre-culture representative samples) and at the end of the test. Fronds were observed for their appearance on days ‘3’, ‘5’ and ‘7’. All fronds were found normal and healthy in all replicates of control and test concentration. The doubling time (Td) of frond number in the control group was calculated as 1.608 day-1.
Frond Numbers:
From the average specific growth rate recorded with the test concentration 100 mg/L the test concentration that inhibited 50% growth (ErC50) was calculated as >100 mg/L. The percent inhibition on the average specific growth rate on day ‘7’ for the test concentration of 100 mg/L was calculated as 0 %. From the yield recorded with the test concentration,100mg/L, the concentration that inhibited 50 % yield (EyC50) was calculated as >100 mg/L. The percent inhibition on the yield on day ‘7’ for the test concentration of 100 mg/L was calculated as 0 %.
Frond Dry Weight:
From the average specific growth rate recorded with the test concentration, 100 mg/L, the concentration that inhibited 50 % growth (ErC50) was calculated as >100 mg/L. The percent inhibition on the average growth rate on day ‘7’ for the concentration of 100 mg/L was calculated as 0.21 %. From the yield recorded with the test concentration,100 mg/L the concentration that inhibited 50 % yield (EyC50) was calculated as >100 mg/L.
The percent inhibition on the yield on day ‘7’ for the test concentration of 100 mg/L was calculated as 0.71 %.
In conclusion:
ErC50 of FAT 20043/E based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L.
EyC50 of FAT 20043/E based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L
ErC50 of FAT 20043/E based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L
EyC50 of FAT 20043/E based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L.
Reference
Description of key information
In a GLP-compliant OECD guideline 221 followed study, Lemna gibba (Duckweed) were exposed to the test item (FAT 20043/E) at test concentration 100 mg/L.Three replicates for each concentration and six replicates as a control were maintained. Observations and frond number counts were made on days ‘0’, ‘3’, ‘5’ and ‘7’.
The following results were observed:
ErC50 of FAT 20043/E based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L.
EyC50 of FAT 20043/E based upon the frond numbers observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L
ErC50 of FAT 20043/E based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L
EyC50 of FAT 20043/E based upon the frond dry weight observed for a period of ‘7’ days for Lemna gibba was found to be >100 mg/L.
All fronds were found normal and healthy in all replicates of control and test concentration after 7 days leading to the end point being determined as ErC50>100mg/Land EyC50>100mg/L.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 100 mg/L
- EC10 or NOEC for freshwater plants:
- 100 mg/L
Additional information
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