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EC number: 259-839-8 | CAS number: 55819-71-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 Dec 2000 - 11 Jan 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Federal Office of Public Health, Bern, Switzerland
Test material
- Reference substance name:
- DL-serinohydrazide monohydrochloride
- EC Number:
- 259-839-8
- EC Name:
- DL-serinohydrazide monohydrochloride
- Cas Number:
- 55819-71-1
- Molecular formula:
- C3H9N3O2.ClH
- IUPAC Name:
- 2-amino-3-hydroxy-propanehydrazide;hydrochloride
- Details on test material:
- - Name of test material (as cited in study report): DL-Serine hydrazide HCl
- Substance type: white solid
- Physical state: solid
- Expiration date of the lot/batch: 06 Dec 2001
- Storage condition of test material: in refrigerator at about 4 °C, under N2
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The study was performed with aerobic activated sludge from a wastewater treatment plant (ARA Ergolz II, Fullinsdorf, Switzerland).
- Preparation of inoculum for exposure: The sludge was washed once with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated. Based on this ratio, calculated amounts of wet sludge were suspended in test water to obtain a concentration equivalent to 4 g (± 10%) dry material per liter. During holding, the sludge was aerated at room temperature until use. Prior to use, the sludge was diluted with test water to a concentration of 1 g per liter (dry weight basis). This diluted activated sludge was used as inoculum to give a final concentration of 30 mg dry material per liter.
- Concentration of sludge: 30 mg dry material/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- other: BOD (biochemical oxygen demand)
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to testing guideline
- Test temperature: 22 °C
- pH: 7.4
- pH adjusted: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 500 mL Erlenmeyer flasks
- Number of culture flasks/concentration: 2
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic control: 1
- Toxicity control: 1
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- other: BOD
- Value:
- 89
- Sampling time:
- 28 d
- Remarks on result:
- other: The percent biodegradation of the test item was calculated based on the theoretical oxygen demand (ThOD, not corrected for nitrification) of 0.41 mg O2/mg test item.
- Details on results:
- The biochemical oxygen demand (BOD) of DL-Serine hydrazide HCI in the test media significantly increased from Day 4 until test-termination after 28 days. At the end of the 28-day exposure period, the mean biodegradation rate of DL-Serine hydrazide HGI amounted to 89%. The pass level for ready biodegradability, i.e. a biodegradation rate of at least 60% of the ThOD in a 10-day window within the 28-day period of the test, was clearly reached. Consequently, DL-Serine hydrazide HCl was found to be readily and completely biodegradable under the test conditions within 28 days.
BOD5 / COD results
- Results with reference substance:
- The percent biodegradation of the reference item sodium benzoate was calculated based on the theoretical oxygen demand of 1.67 mg O2/mg (ThOD). In the procedure controls, the reference item was degraded by an average of 88% by exposure Day 14, thus confirming suitability of the activated sludge. At the end of the test (Day 28), the reference item was degraded by an average of 92%.
No degradation of the test item occurred in the abiotic control under the test conditions.
The percent biodegradation in the toxicity control containing both the test item and the reference item was calculated based on the ThOD of the test item and the reference item. The biodegradation rate in the toxicity control showed a similar course of biodegradation over the 28-day exposure period as the two procedure controls containing the reference item only. Within 14 days of exposure, a biodegradation rate of 87% was observed. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms because the biodegradation rate in the toxicity control was >25% within 14 days.
Any other information on results incl. tables
Table 1: Cumulative biochemical oxygen demand (BOD mg O2/L) in the test flasks
|
Test item |
Inoculum control |
Procedure control |
Abiotic control |
Toxicity control |
|||
Time (days) |
Flask number |
Flask number |
Flask number |
Flask number |
Flask number |
|||
|
7 |
8 |
1 |
2 |
11 |
12 |
9 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
12 |
0 |
0 |
2 |
- |
- |
- |
- |
- |
- |
- |
- |
3 |
- |
- |
- |
- |
- |
- |
- |
- |
4 |
10 |
9 |
0 |
0 |
121 |
120 |
0 |
135 |
5 |
14 |
14 |
0 |
0 |
133 |
131 |
0 |
147 |
6 |
18 |
19 |
0 |
0 |
137 |
135 |
0 |
154 |
7 |
22 |
23 |
0 |
0 |
141 |
139 |
0 |
161 |
8 |
26 |
26 |
0 |
0 |
143 |
139 |
0 |
165 |
9 |
- |
- |
- |
- |
- |
- |
- |
- |
10 |
- |
- |
- |
- |
- |
- |
- |
- |
11 |
34 |
32 |
3 |
0 |
149 |
143 |
0 |
177 |
12 |
- |
- |
- |
- |
- |
- |
- |
- |
13 |
36 |
34 |
4 |
0 |
152 |
145 |
0 |
181 |
14 |
36 |
35 |
4 |
0 |
154 |
145 |
0 |
183 |
15 |
36 |
35 |
4 |
0 |
154 |
145 |
0 |
183 |
16 |
- |
- |
- |
- |
- |
- |
- |
- |
17 |
- |
- |
- |
- |
- |
- |
- |
- |
18 |
40 |
37 |
5 |
0 |
159 |
145 |
0 |
188 |
19 |
40 |
37 |
6 |
0 |
160 |
145 |
0 |
190 |
20 |
40 |
37 |
6 |
0 |
161 |
145 |
0 |
191 |
21 |
40 |
37 |
6 |
0 |
162 |
145 |
0 |
192 |
22 |
42 |
37 |
7 |
0 |
163 |
145 |
0 |
194 |
23 |
- |
- |
- |
- |
- |
- |
- |
- |
24 |
- |
- |
- |
- |
- |
- |
- |
- |
25 |
44 |
38 |
7 |
0 |
165 |
145 |
0 |
200 |
26 |
44 |
38 |
8 |
0 |
166 |
146 |
0 |
201 |
27 |
44 |
38 |
8 |
0 |
167 |
147 |
0 |
204 |
28 |
44 |
38 |
9 |
0 |
168 |
148 |
0 |
206 |
- = no reading taken
Table 2: Biodegradation of DL-Serine hydrazide HCl and the reference item
Time (days) |
Percentage Biodegradation* |
||||
Test item |
Procedure control |
Toxicity control |
|||
Flask No. 7 |
Flask No. 8 |
Flask No. 11 |
Flask No. 12 |
Flask No. 10 |
|
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
7 |
0 |
2 |
- |
- |
- |
- |
- |
3 |
- |
- |
- |
- |
- |
4 |
24 |
22 |
72 |
72 |
65 |
5 |
34 |
34 |
80 |
78 |
71 |
6 |
44 |
46 |
82 |
81 |
74 |
7 |
54 |
56 |
84 |
83 |
77 |
8 |
63 |
63 |
86 |
83 |
79 |
9 |
- |
- |
- |
- |
- |
10 |
- |
- |
- |
- |
- |
11 |
79 |
74 |
88 |
85 |
84 |
12 |
- |
- |
- |
- |
- |
13 |
83 |
78 |
90 |
86 |
86 |
14 |
83 |
80 |
91 |
86 |
87 |
15 |
83 |
80 |
91 |
86 |
87 |
16 |
- |
- |
- |
- |
- |
17 |
- |
- |
- |
- |
- |
18 |
91 |
84 |
94 |
85 |
89 |
19 |
90 |
83 |
94 |
85 |
90 |
20 |
90 |
83 |
95 |
85 |
90 |
21 |
90 |
83 |
95 |
85 |
91 |
22 |
94 |
82 |
96 |
85 |
92 |
23 |
- |
- |
- |
- |
- |
24 |
- |
- |
- |
- |
- |
25 |
99 |
84 |
97 |
85 |
94 |
26 |
98 |
83 |
97 |
85 |
95 |
27 |
98 |
83 |
98 |
86 |
96 |
28 |
96 |
82 |
98 |
86 |
97 |
Mean (Day 28) |
89 |
92 |
|
* = Corrected for the inoculum controls
- = not determined
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the conditions of the study the test item was readily biodegradable. The biochemical oxygen demand at the end of the 28-day exposure period amounted to 89%. The pass level for ready biodegradability, i.e. a biodegradation rate of at least 60% of the ThOD in a 10-day window within the 28-day period of the test, was clearly reached. All validity criteria were fulfilled.
- Executive summary:
The test item DL-Serine hydrazide HCI was investigated for its ready biodegradability in a 28-Day Manometric Respirometry Test based on Directive 92/69 EEC, C.4-D, 1992 and OECD Guideline for Testing of Chemicals No. 301 F, 1992.
The nitrite and nitrate concentrations in the test media and in the inoculum controls were in the same low range at the end of the test. Consequently, no significant nitrification had occurred in the test media with DL-Serine hydrazide HCI during the degradation test period. Therefore, the measured biological oxygen demand is caused only by the biodegradation of the test item. For that reason, the percent biodegradation of the test item was calculated based on the theoretical oxygen demand ThOD NH4 of 0.41 mg O2/mg test item (without taking nitrification into account).
The biochemical oxygen demand (BOD) of DL-Serine hydrazide HCI in the test media significantly increased from Day 4 until test-termination after 28 days. At the end of the 28-day exposure period, the mean biodegradation rate of DL-Serine hydrazide HCI amounted to 89%.
The pass level for ready biodegradability, i.e. a biodegradation rate of at least 60% of the ThOD in a 10-day window within the 28-day period of the test, was clearly reached. Consequently, DL-Serine hydrazide HCI was found to be ready and completely biodegradable under the test conditions within 28 days.
In the toxicity control, containing both DL-Serine hydrazide HCI and the reference item sodium benzoate, no inhibitory effect on the biodegradation of the reference item was determined. Thus, DL-Serine hydrazide HCI had obviously no inhibitory effect on the activity of activated sludge microorganisms.
In the procedure controls, the reference item sodium benzoate was biodegraded by an average of 88% on exposure Day 14, and reached an average biodegradation rate of 92% by the end of the test (Day 28), thus confirming suitability of the activated sludge.
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