Butyl cyanoacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981-01-28

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable for reliability but not in detail documented. Study report meets basic scientific principles. Study was conducted prior to GLP and OECD guideline implementation.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

No further information available.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Butyl cyanoacetate

EC Number:

226-730-1

EC Name:

Butyl cyanoacetate

Cas Number:

5459-58-5

Molecular formula:

C7H11NO2

IUPAC Name:

butyl 2-cyanoacetate

Test material form:

other: liquid

Details on test material:

Identification: Cyanessigsäurebutylester
Appearance: liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

3.1 mg/kg
5.0 mg/kg
5.5 mg/kg
6.2 mg/kg
7.2 mg/kg

No. of animals per sex per dose:

5 male and 5 female were used for each dose level.

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

4.97 mL/kg bw

Based on:

test mat.

95% CL:

3.68 - 5.36

Any other information on results incl. tables

At Single dose of 5.0 - 7.2 mL/kg/bw all male and female rats had the following symptoms: Unsteady locomotion, hemiplegia of the hind limbs, unkempt coats, loss of weights and a general loss of activity. Animals dosed from level 5.5 up to dose level 7.2 mL/kg/bw additional symptoms were observed such as lateral and ventral position of the animals. The symptoms occured one hour after administration and were evident until end of the study. Mortality was noted from 2. until 12. study day. All findings are applicable for male and female rats. No significant differences between both sexes.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The calculated LD50 value of Butyl cyanoacetate is 4.97 mL/kg/bw (confidence interval for p < 0.05 = 3.68 - 5.36 mL/kg/bw). Therefore, the test item will be not classified as acute toxic according to GHS/CLP classification criteria..

Executive summary:

The study was performed 1981, before GLP- and OECD-testing guidelines were available and in force.

The calculated LD50 value of Butyl cyanoacetate is 4.97 mL/kg/bw (confidence interval for p < 0.05 = 3.68 - 5.36 mL/kg/bw). Therefore, the test item will be not classified as acute toxic according to GHS/CLP classification criteria..