Plastic, waste, pyrolized, fractionation bottoms

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Limit test:

yes

Total exposure duration:

96 h

Hardness:

Day 0: 89 mg CaCO3/L; 5NO3 in mg/L

Test temperature:

23.3-24.4

pH:

7.6-7.8

Dissolved oxygen:

7.5-8.6 mg/l

Nominal and measured concentrations:

nominal load of 500 mg/L

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 7.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Validity criteria fulfilled:

yes

Conclusions:

The toxicity against Danio rerio was tested using a static test design. The control and the
tested concentration showed no mortality. No abnormal behaviour of the surviving fish was
observed.
The content of the test item in the test solutions was estimated by calculation based on the
carbon content of the test item and TOC measurement. The measured concentration in the
test solutions was 9.0 mg/L at the beginning and 6.9 mg/L at the end of the test.
Therefore, the geometric mean of the measured concentration (7.9 mg/L) was used for evaluation
of the results.
No observations were made which might cause doubts concerning the validity of the study
outcome. All validity criteria were met.
The result of the test is considered valid.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

> 7.9 mg/L